Long-term stability of frozen platelet-rich plasma under −80 °C storage condition
Platelet rich plasma (PRP) is increasingly used in various fields of medicine, aiming to regeneration and repair damaged tissues, cells and organs. High concentration of bioactive molecules including growth factors, cytokines and chemokines are the rationale of using PRP. The aim of this study is to...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001676 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846148920868077568 |
|---|---|
| author | Wanbing Liu Yan Liu Tao Li Lei Liu Mei Du Jinbing Du Yong Qi Guangda Xiang |
| author_facet | Wanbing Liu Yan Liu Tao Li Lei Liu Mei Du Jinbing Du Yong Qi Guangda Xiang |
| author_sort | Wanbing Liu |
| collection | DOAJ |
| description | Platelet rich plasma (PRP) is increasingly used in various fields of medicine, aiming to regeneration and repair damaged tissues, cells and organs. High concentration of bioactive molecules including growth factors, cytokines and chemokines are the rationale of using PRP. The aim of this study is to analyze the effect of frozen on the levels of growth factors. In our study, PRP samples were isolated from 50 healthy volunteers using the Trima Accel blood cell separator. The concentration of growth factors such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF-1) and platelet factor 4 (PF-4) were assessed in fresh PRP and frozen PRP stored at −80 °C for one to twelve months. The study found that count of platelet in all fresh and frozen PRP samples was significantly increased compared to whole blood baseline. There was no significant difference in the concentrations of PDGF-BB, bFGF, VEGF, and PF-4 between fresh and frozen samples. The concentrations of EGF and IGF in Frozen-PRP group were significantly higher than those in Fresh-PRP group. And the storage condition of −80 °C is suitable for PRP, which will not lead to a decrease in growth factors concentration for at least 6 months. |
| format | Article |
| id | doaj-art-e9d578d89dc040dd9d0d7a6481b23a96 |
| institution | Kabale University |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-e9d578d89dc040dd9d0d7a6481b23a962024-11-30T07:11:02ZengElsevierRegenerative Therapy2352-32042024-06-0126826830Long-term stability of frozen platelet-rich plasma under −80 °C storage conditionWanbing Liu0Yan Liu1Tao Li2Lei Liu3Mei Du4Jinbing Du5Yong Qi6Guangda Xiang7Department of Transfusion, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, ChinaDepartment of Clinical Laboratory, Maternal and Child Health Hospital of Hubei Province, Wuluo Road 745, Wuhan 430070, Hubei Province, ChinaDepartment of Endocrinology, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, ChinaDepartment of Transfusion, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, ChinaThe Second Department of Gynecology, Maternal and Child Health Hospital of Hubei Province, Wuluo Road 745, Wuhan 430070, Hubei Province, ChinaDepartment of Transfusion, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, ChinaDepartment of Transfusion, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, ChinaDepartment of Endocrinology, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, China; Corresponding author.Platelet rich plasma (PRP) is increasingly used in various fields of medicine, aiming to regeneration and repair damaged tissues, cells and organs. High concentration of bioactive molecules including growth factors, cytokines and chemokines are the rationale of using PRP. The aim of this study is to analyze the effect of frozen on the levels of growth factors. In our study, PRP samples were isolated from 50 healthy volunteers using the Trima Accel blood cell separator. The concentration of growth factors such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF-1) and platelet factor 4 (PF-4) were assessed in fresh PRP and frozen PRP stored at −80 °C for one to twelve months. The study found that count of platelet in all fresh and frozen PRP samples was significantly increased compared to whole blood baseline. There was no significant difference in the concentrations of PDGF-BB, bFGF, VEGF, and PF-4 between fresh and frozen samples. The concentrations of EGF and IGF in Frozen-PRP group were significantly higher than those in Fresh-PRP group. And the storage condition of −80 °C is suitable for PRP, which will not lead to a decrease in growth factors concentration for at least 6 months.http://www.sciencedirect.com/science/article/pii/S2352320424001676Platelet rich plasmaFrozen storagePlatelet-derived growth factor (PDGF)Vascular endothelial growth factor (VEGF)Epidermal growth factor (EGF)Basic fibroblast growth factor (bFGF) |
| spellingShingle | Wanbing Liu Yan Liu Tao Li Lei Liu Mei Du Jinbing Du Yong Qi Guangda Xiang Long-term stability of frozen platelet-rich plasma under −80 °C storage condition Regenerative Therapy Platelet rich plasma Frozen storage Platelet-derived growth factor (PDGF) Vascular endothelial growth factor (VEGF) Epidermal growth factor (EGF) Basic fibroblast growth factor (bFGF) |
| title | Long-term stability of frozen platelet-rich plasma under −80 °C storage condition |
| title_full | Long-term stability of frozen platelet-rich plasma under −80 °C storage condition |
| title_fullStr | Long-term stability of frozen platelet-rich plasma under −80 °C storage condition |
| title_full_unstemmed | Long-term stability of frozen platelet-rich plasma under −80 °C storage condition |
| title_short | Long-term stability of frozen platelet-rich plasma under −80 °C storage condition |
| title_sort | long term stability of frozen platelet rich plasma under 80 °c storage condition |
| topic | Platelet rich plasma Frozen storage Platelet-derived growth factor (PDGF) Vascular endothelial growth factor (VEGF) Epidermal growth factor (EGF) Basic fibroblast growth factor (bFGF) |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001676 |
| work_keys_str_mv | AT wanbingliu longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT yanliu longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT taoli longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT leiliu longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT meidu longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT jinbingdu longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT yongqi longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition AT guangdaxiang longtermstabilityoffrozenplateletrichplasmaunder80cstoragecondition |