Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process
The bacterial cellulose-producing strains were isolated and screened from waste tobacco leaves, and the strains were identified by morphologic observation, physiological and biochemical tests and molecular biological identification. Using waste tobacco extract as carbon and nitrogen source, the mixe...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-09-01
|
| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-9-92.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850044051094503424 |
|---|---|
| author | HUANG Lei, LI Yuanyuan, HE Aimin, CHEN Weihua, WANG Xiaoru, CUI Bing, LAI Miao, ZHAO Mingqin |
| author_facet | HUANG Lei, LI Yuanyuan, HE Aimin, CHEN Weihua, WANG Xiaoru, CUI Bing, LAI Miao, ZHAO Mingqin |
| author_sort | HUANG Lei, LI Yuanyuan, HE Aimin, CHEN Weihua, WANG Xiaoru, CUI Bing, LAI Miao, ZHAO Mingqin |
| collection | DOAJ |
| description | The bacterial cellulose-producing strains were isolated and screened from waste tobacco leaves, and the strains were identified by morphologic observation, physiological and biochemical tests and molecular biological identification. Using waste tobacco extract as carbon and nitrogen source, the mixed-strains fermentation was carried out with screened strain and Saccharomyces cerevisiae (1∶1), and the effects of waste tobacco extract addition, mixed-strains inoculum, pH, fermentation temperature and time on the yield of bacterial cellulose were investigated. The results showed that the fermentation process conditions were optimized by single factor, Plackett-Burman and Box-Behnken experiments. The results showed that a bacterial cellulose-producing strain YC1 was selected and identified as Gluconobacter oxydans. The optimal mixed-strains fermentation process conditions were as follows: waste tobacco extract addition 10%, strain YC1 and S. cerevisiae (1∶1) inoculum 1.0%, initial pH 5.5, fermentation temperature 30 ℃ and time 6 d. Under these optimal process conditions, the bacterial cellulose yield was the highest (2.71 g/L), which was 1.29 times higher than that of strain YC1 fermentation alone (1.18 g/L). |
| format | Article |
| id | doaj-art-e9d55ff0ae83411fbb67e1a88b8c6063 |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-e9d55ff0ae83411fbb67e1a88b8c60632025-08-20T02:55:05ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-09-01439929810.11882/j.issn.0254-5071.2024.09.014Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation processHUANG Lei, LI Yuanyuan, HE Aimin, CHEN Weihua, WANG Xiaoru, CUI Bing, LAI Miao, ZHAO Mingqin01. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450046, China;;2. China Tobacco Hebei Industrial Co., Ltd., Shijiazhuang 050051, ChinaThe bacterial cellulose-producing strains were isolated and screened from waste tobacco leaves, and the strains were identified by morphologic observation, physiological and biochemical tests and molecular biological identification. Using waste tobacco extract as carbon and nitrogen source, the mixed-strains fermentation was carried out with screened strain and Saccharomyces cerevisiae (1∶1), and the effects of waste tobacco extract addition, mixed-strains inoculum, pH, fermentation temperature and time on the yield of bacterial cellulose were investigated. The results showed that the fermentation process conditions were optimized by single factor, Plackett-Burman and Box-Behnken experiments. The results showed that a bacterial cellulose-producing strain YC1 was selected and identified as Gluconobacter oxydans. The optimal mixed-strains fermentation process conditions were as follows: waste tobacco extract addition 10%, strain YC1 and S. cerevisiae (1∶1) inoculum 1.0%, initial pH 5.5, fermentation temperature 30 ℃ and time 6 d. Under these optimal process conditions, the bacterial cellulose yield was the highest (2.71 g/L), which was 1.29 times higher than that of strain YC1 fermentation alone (1.18 g/L).https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-9-92.pdfbacterial cellulose-producing strains|screening and identification|waste tobacco leaves|mixed-strains fermentation process|response surface optimization |
| spellingShingle | HUANG Lei, LI Yuanyuan, HE Aimin, CHEN Weihua, WANG Xiaoru, CUI Bing, LAI Miao, ZHAO Mingqin Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process Zhongguo niangzao bacterial cellulose-producing strains|screening and identification|waste tobacco leaves|mixed-strains fermentation process|response surface optimization |
| title | Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process |
| title_full | Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process |
| title_fullStr | Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process |
| title_full_unstemmed | Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process |
| title_short | Screening and identification of bacterial cellulose-producing strains from waste tobacco leaves and optimization of mixed-strains fermentation process |
| title_sort | screening and identification of bacterial cellulose producing strains from waste tobacco leaves and optimization of mixed strains fermentation process |
| topic | bacterial cellulose-producing strains|screening and identification|waste tobacco leaves|mixed-strains fermentation process|response surface optimization |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-9-92.pdf |
| work_keys_str_mv | AT huangleiliyuanyuanheaiminchenweihuawangxiaorucuibinglaimiaozhaomingqin screeningandidentificationofbacterialcelluloseproducingstrainsfromwastetobaccoleavesandoptimizationofmixedstrainsfermentationprocess |