Genetic Dissection of the Powdery Mildew Resistance in a Cultivated Emmer Wheat Accession

Genetic Dissection of the Powdery Mildew Resistance in a Cultivated Emmer Wheat Accession

<i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), the causal agent of wheat powdery mildew, poses a significant threat to global wheat production. In this study, we identified and characterized a broad-spectrum powdery mildew resistance gene, <i>P...

Full description

Saved in:
Bibliographic Details
Main Authors: Ruishan Liu, Yuli Jin, Ningning Yu, Hongxing Xu, Xusheng Sun, Jiangchun Wang, Xueqing Liu, Jiadong Zhang, Jiatong Li, Yaoxue Li, Pengtao Ma
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Agronomy
Subjects:
cultivated emmer wheat
powdery mildew
molecular detection
BSR-seq analysis
expression patterns
Online Access:https://www.mdpi.com/2073-4395/15/4/980
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), the causal agent of wheat powdery mildew, poses a significant threat to global wheat production. In this study, we identified and characterized a broad-spectrum powdery mildew resistance gene, <i>PmL709</i>, in a resistant cultivated emmer wheat (<i>Triticum dicoccum</i>) accession: L709. Using bulked segregant RNA sequencing (BSR-Seq) analysis and molecular markers, <i>PmL709</i> was mapped to a 1.7 cM interval on chromosome arm 2BS, flanked by markers <i>Xdw05</i>/<i>YTU95-04</i>/<i>YTU95-06</i>/<i>YTU95-08</i>/<i>Xdw10</i>/<i>Xdw11</i> and <i>YTU692B-094</i>, corresponding to a 21.82–25.94 Mb physical interval (cv. Svevo), using the segregated population crossed by L709 and a susceptible durum wheat cultivar, Langdon. Referring to the origin, the resistance spectra, and the physical position with known resistance genes on chromosome arm 2BS, <i>PmL709</i> was likely to be an allele of <i>Pm68</i>. Transcriptomic analysis revealed 3923 differentially expressed genes (DEGs) between resistant and susceptible bulks, enriched in pathways such as phenylpropanoid biosynthesis, MAPK signaling, and plant–pathogen interactions. qRT-PCR validated the differential expression of nine candidate genes within the <i>PmL709</i> interval, highlighting their potential roles in disease resistance. The flanking markers could accurately trace the presence of <i>PmL709</i> from resistant accession L709 in a survey of 46 susceptible wheat accessions. These findings provide valuable insights into the genetic and molecular mechanisms of powdery mildew resistance in wheat and offer practical tools for marker-assisted breeding to develop resistant cultivars.
ISSN:2073-4395

Similar Items