Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination

Summary: Multiple sclerosis (MS) is initially characterized by myelin and axonal damage in central nervous system white matter lesions, but their causal role in synapse loss remains undefined. Gray matter atrophy is also present early in MS, making it unclear if synaptic alterations are driven by wh...

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Main Authors: Gabrielle M. Mey, Kirsten S. Evonuk, John Shelestak, Muhammad Irfan, Laura M. Wolfe, Sophia E. Laye, Dorothy P. Schafer, Tara M. DeSilva
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:iScience
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Online Access:http://www.sciencedirect.com/science/article/pii/S2589004224024519
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author Gabrielle M. Mey
Kirsten S. Evonuk
John Shelestak
Muhammad Irfan
Laura M. Wolfe
Sophia E. Laye
Dorothy P. Schafer
Tara M. DeSilva
author_facet Gabrielle M. Mey
Kirsten S. Evonuk
John Shelestak
Muhammad Irfan
Laura M. Wolfe
Sophia E. Laye
Dorothy P. Schafer
Tara M. DeSilva
author_sort Gabrielle M. Mey
collection DOAJ
description Summary: Multiple sclerosis (MS) is initially characterized by myelin and axonal damage in central nervous system white matter lesions, but their causal role in synapse loss remains undefined. Gray matter atrophy is also present early in MS, making it unclear if synaptic alterations are driven by white matter demyelinating lesions or primary gray matter damage. Furthermore, whether axonal pathology occurs secondary to or independent of demyelination to drive synaptic changes is not clear. Here, we address whether reducing demyelination by selectively manipulating glutamatergic signaling in mature oligodendrocytes (OLs) preserves synapses in experimental autoimmune encephalomyelitis (EAE), a preclinical model of demyelinating disease. We demonstrate that inducible reduction of the GluA4 AMPA-type glutamate receptor subunit selectively in mature (OLs) reduces demyelination and axonal injury, preserves synapses, and improves visual function during EAE. These data link demyelination to the pathophysiology of synaptic loss with therapeutic implications for both motor and cognitive disability in MS.
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series iScience
spelling doaj-art-e9d006bcbc3e4c3888f9df711499d5082025-08-20T02:12:42ZengElsevieriScience2589-00422024-11-01271111122610.1016/j.isci.2024.111226Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelinationGabrielle M. Mey0Kirsten S. Evonuk1John Shelestak2Muhammad Irfan3Laura M. Wolfe4Sophia E. Laye5Dorothy P. Schafer6Tara M. DeSilva7Department of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USADepartment of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USADepartment of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USADepartment of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USADepartment of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USADepartment of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USADepartment of Neurobiology, Brudnik Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, MA, USADepartment of Neurosciences, Cleveland Clinic and Case Western Reserve University, Cleveland, OH, USA; Corresponding authorSummary: Multiple sclerosis (MS) is initially characterized by myelin and axonal damage in central nervous system white matter lesions, but their causal role in synapse loss remains undefined. Gray matter atrophy is also present early in MS, making it unclear if synaptic alterations are driven by white matter demyelinating lesions or primary gray matter damage. Furthermore, whether axonal pathology occurs secondary to or independent of demyelination to drive synaptic changes is not clear. Here, we address whether reducing demyelination by selectively manipulating glutamatergic signaling in mature oligodendrocytes (OLs) preserves synapses in experimental autoimmune encephalomyelitis (EAE), a preclinical model of demyelinating disease. We demonstrate that inducible reduction of the GluA4 AMPA-type glutamate receptor subunit selectively in mature (OLs) reduces demyelination and axonal injury, preserves synapses, and improves visual function during EAE. These data link demyelination to the pathophysiology of synaptic loss with therapeutic implications for both motor and cognitive disability in MS.http://www.sciencedirect.com/science/article/pii/S2589004224024519Cell biologyImmunologyNeuroscience
spellingShingle Gabrielle M. Mey
Kirsten S. Evonuk
John Shelestak
Muhammad Irfan
Laura M. Wolfe
Sophia E. Laye
Dorothy P. Schafer
Tara M. DeSilva
Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
iScience
Cell biology
Immunology
Neuroscience
title Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
title_full Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
title_fullStr Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
title_full_unstemmed Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
title_short Inhibiting AMPA receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
title_sort inhibiting ampa receptor signaling in oligodendrocytes rescues synapse loss in a model of autoimmune demyelination
topic Cell biology
Immunology
Neuroscience
url http://www.sciencedirect.com/science/article/pii/S2589004224024519
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