Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum
Strawberries (Fragaria ananassa) are highly perishable due to fungal spoilage, limiting their shelf life. This study evaluated the antifungal potential of five lactic acid bacteria (LAB) strains against Botrytis cinerea, Rhizopus stolonifer, and Aspergillus niger isolated from strawberries, along wi...
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Taylor & Francis Group
2025-12-01
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| Series: | CyTA - Journal of Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2025.2541890 |
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| author | Angie Lizeth Quintero Pinilla Esteban Florez Jaramillo Anna María Polanía Rivera Cristina Ramírez Toro German Armando Bolívar Escobar |
| author_facet | Angie Lizeth Quintero Pinilla Esteban Florez Jaramillo Anna María Polanía Rivera Cristina Ramírez Toro German Armando Bolívar Escobar |
| author_sort | Angie Lizeth Quintero Pinilla |
| collection | DOAJ |
| description | Strawberries (Fragaria ananassa) are highly perishable due to fungal spoilage, limiting their shelf life. This study evaluated the antifungal potential of five lactic acid bacteria (LAB) strains against Botrytis cinerea, Rhizopus stolonifer, and Aspergillus niger isolated from strawberries, along with their biosurfactant and organic acid production. Antifungal activity was assessed by measuring inhibition radio (mm) around LAB-containing agar disks after incubation at 30°C for 2, 3, and 7 days. Lactiplantibacillus plantarum showed strong antifungal activity, inhibiting B. cinerea (14.44 ± 0.30 mm) and A. niger (50.03 ± 0.45 mm) after 7 days, and preventing R. stolonifer sporulation (22.90 ± 1.20 mm) within 3 days. Results of the in vivo antifungal activity conducted over 7 days demonstrated L. plantarum ability to prevent the growth of B. cinerea and R. stolonifer and reduce the invasion of A. niger. These results highlight L. plantarum as a promising natural biocontrol agent to extend strawberry shelf life and reduce postharvest losses. |
| format | Article |
| id | doaj-art-e9cd8bdc28de4b798e2a23fde4fc9299 |
| institution | Kabale University |
| issn | 1947-6337 1947-6345 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj-art-e9cd8bdc28de4b798e2a23fde4fc92992025-08-20T03:59:31ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452025-12-0123110.1080/19476337.2025.2541890Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarumAngie Lizeth Quintero Pinilla0Esteban Florez Jaramillo1Anna María Polanía Rivera2Cristina Ramírez Toro3German Armando Bolívar Escobar4Faculty of Engineering, School of Food Engineering, Universidad del Valle (University of Valle), Tuluá Valle del Cauca, ColombiaFaculty of Engineering, School of Food Engineering, Universidad del Valle (University of Valle), Tuluá Valle del Cauca, ColombiaFaculty of Engineering, School of Food Engineering, Universidad del Valle (University of Valle), Tuluá Valle del Cauca, ColombiaFaculty of Engineering, School of Food Engineering, Universidad del Valle (University of Valle), Cali, Valle del Cauca, ColombiaDepartment of Biology, Faculty of Natural and Exact Sciences, Universidad del Valle (University of Valle), Cali, Valle del Cauca, ColombiaStrawberries (Fragaria ananassa) are highly perishable due to fungal spoilage, limiting their shelf life. This study evaluated the antifungal potential of five lactic acid bacteria (LAB) strains against Botrytis cinerea, Rhizopus stolonifer, and Aspergillus niger isolated from strawberries, along with their biosurfactant and organic acid production. Antifungal activity was assessed by measuring inhibition radio (mm) around LAB-containing agar disks after incubation at 30°C for 2, 3, and 7 days. Lactiplantibacillus plantarum showed strong antifungal activity, inhibiting B. cinerea (14.44 ± 0.30 mm) and A. niger (50.03 ± 0.45 mm) after 7 days, and preventing R. stolonifer sporulation (22.90 ± 1.20 mm) within 3 days. Results of the in vivo antifungal activity conducted over 7 days demonstrated L. plantarum ability to prevent the growth of B. cinerea and R. stolonifer and reduce the invasion of A. niger. These results highlight L. plantarum as a promising natural biocontrol agent to extend strawberry shelf life and reduce postharvest losses.https://www.tandfonline.com/doi/10.1080/19476337.2025.2541890BiocontrolstrawberryfungiLactiplantibacillus plantarumantifungal |
| spellingShingle | Angie Lizeth Quintero Pinilla Esteban Florez Jaramillo Anna María Polanía Rivera Cristina Ramírez Toro German Armando Bolívar Escobar Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum CyTA - Journal of Food Biocontrol strawberry fungi Lactiplantibacillus plantarum antifungal |
| title | Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum |
| title_full | Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum |
| title_fullStr | Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum |
| title_full_unstemmed | Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum |
| title_short | Isolation and identification of three fungi present in strawberry (Fragaria ananassa) and in vitro evaluation of the antifungal activity of Lactiplantibacillus plantarum |
| title_sort | isolation and identification of three fungi present in strawberry fragaria ananassa and in vitro evaluation of the antifungal activity of lactiplantibacillus plantarum |
| topic | Biocontrol strawberry fungi Lactiplantibacillus plantarum antifungal |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2025.2541890 |
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