High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells
Abstract The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57537-8 |
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| author | Camilla Rega Ifigenia Tsitsa Theodoros I. Roumeliotis Izabella Krystkowiak Maria Portillo Lu Yu Julia Vorhauser Jonathon Pines Jörg Mansfeld Jyoti Choudhary Norman E. Davey |
| author_facet | Camilla Rega Ifigenia Tsitsa Theodoros I. Roumeliotis Izabella Krystkowiak Maria Portillo Lu Yu Julia Vorhauser Jonathon Pines Jörg Mansfeld Jyoti Choudhary Norman E. Davey |
| author_sort | Camilla Rega |
| collection | DOAJ |
| description | Abstract The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1 cells using deep quantitative mass spectrometry by isobaric labelling. By analysing non-transformed cells and improving the temporal resolution and coverage of key cell cycle regulators, we present a dataset of cell cycle-dependent protein and phosphorylation site oscillation that offers a foundational reference for investigating cell cycle regulation. These data reveal regulatory intricacies including proteins and phosphorylation sites exhibiting cell cycle-dependent oscillation, and proteins targeted for degradation during mitotic exit. Integrated with complementary resources, our data link cycle-dependent abundance dynamics to functional changes and are accessible through the Cell Cycle database (CCdb), an interactive web-based resource for the cell cycle community. |
| format | Article |
| id | doaj-art-e9cb99e71d0944f6870e32d6de20266a |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e9cb99e71d0944f6870e32d6de20266a2025-08-20T03:01:41ZengNature PortfolioNature Communications2041-17232025-03-0116111810.1038/s41467-025-57537-8High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cellsCamilla Rega0Ifigenia Tsitsa1Theodoros I. Roumeliotis2Izabella Krystkowiak3Maria Portillo4Lu Yu5Julia Vorhauser6Jonathon Pines7Jörg Mansfeld8Jyoti Choudhary9Norman E. Davey10Division of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchDivision of Cancer Biology, The Institute of Cancer ResearchAbstract The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1 cells using deep quantitative mass spectrometry by isobaric labelling. By analysing non-transformed cells and improving the temporal resolution and coverage of key cell cycle regulators, we present a dataset of cell cycle-dependent protein and phosphorylation site oscillation that offers a foundational reference for investigating cell cycle regulation. These data reveal regulatory intricacies including proteins and phosphorylation sites exhibiting cell cycle-dependent oscillation, and proteins targeted for degradation during mitotic exit. Integrated with complementary resources, our data link cycle-dependent abundance dynamics to functional changes and are accessible through the Cell Cycle database (CCdb), an interactive web-based resource for the cell cycle community.https://doi.org/10.1038/s41467-025-57537-8 |
| spellingShingle | Camilla Rega Ifigenia Tsitsa Theodoros I. Roumeliotis Izabella Krystkowiak Maria Portillo Lu Yu Julia Vorhauser Jonathon Pines Jörg Mansfeld Jyoti Choudhary Norman E. Davey High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells Nature Communications |
| title | High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells |
| title_full | High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells |
| title_fullStr | High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells |
| title_full_unstemmed | High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells |
| title_short | High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells |
| title_sort | high resolution profiling of cell cycle dependent protein and phosphorylation abundance changes in non transformed cells |
| url | https://doi.org/10.1038/s41467-025-57537-8 |
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