Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand
<b>Background/Objective:</b><i>Staphylococcus aureus</i> produces staphyloferrin A (Sfna) siderophores to sequester host iron during infection and rapid cell proliferation We examined drug susceptibility, siderophore production, and genome sequencing of clinical isolates of &...
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2025-05-01
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| author | Warinda Prommachote Manu Deeudom Pimpisid Koonyosying Phronpawee Srichomphoo Ratchanee Somnabut Phadungkiat Khamnoi Agostino Cilibrizzi Yuvaraj Ravikumar Somdet Srichairatanakool |
| author_facet | Warinda Prommachote Manu Deeudom Pimpisid Koonyosying Phronpawee Srichomphoo Ratchanee Somnabut Phadungkiat Khamnoi Agostino Cilibrizzi Yuvaraj Ravikumar Somdet Srichairatanakool |
| author_sort | Warinda Prommachote |
| collection | DOAJ |
| description | <b>Background/Objective:</b><i>Staphylococcus aureus</i> produces staphyloferrin A (Sfna) siderophores to sequester host iron during infection and rapid cell proliferation We examined drug susceptibility, siderophore production, and genome sequencing of clinical isolates of <i>S. aureus</i>. <b>Methods:</b> A total of 100 specimens, including pus, sputum, hemoculture, urine, tissue, fluid, and skin scrap specimens, were grown in iron-deprived Luria broth agar. The isolates were investigated for spectral signature using MALDI–TOF/MS, while antibiotic susceptibility and siderophore content were assessed using the chrome azurol S method. Whole genome and partial 16S rRNA DNA sequences were employed, and VITEK/MS revealed specific spectra. <b>Results:</b> Clindamycin, erythromycin, gentamicin, linezolid, moxifloxacin, oxacillin, trimethoprim/sulfamethoxazole, and vancomycin (100%) were the most common antibiotics to which the <i>S. aureus</i> isolates were susceptible. Sfna was not detectable in fluid and skin scrap isolates, which were encoded by <i>sfnaB</i>, <i>sfnaD</i>, and <i>sfnaB</i>/<i>sfnaD</i> genes. However, they were detectable in pus (73.8%), sputum (85.3%), hemoculture (50.0%), and urine (85.7%) isolates. The aureus subspecies, JKD6159, SA268, and MN8, were found to be 72.73% according to genome sequencing. <b>Conclusion:</b> most staphylococci in the isolates, including <i>S. aureus</i> JKD6159, SA268, and MN8, were sensitive to antibiotics and were detected by MALDI–TOF/MS, resulting in the production of Sfna encoded by <i>sfna</i> genes. |
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| spelling | doaj-art-e9ca9d7b0a47430a9f3ed7c67ce230152025-08-20T01:56:20ZengMDPI AGAntibiotics2079-63822025-05-0114552110.3390/antibiotics14050521Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, ThailandWarinda Prommachote0Manu Deeudom1Pimpisid Koonyosying2Phronpawee Srichomphoo3Ratchanee Somnabut4Phadungkiat Khamnoi5Agostino Cilibrizzi6Yuvaraj Ravikumar7Somdet Srichairatanakool8Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Bacteriology, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandClinical Microbiology Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandClinical Microbiology Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandInstitute of Pharmaceutical Sciences, King’s College London, London SE1 9NH, UKDepartment of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Karnataka 560170, IndiaDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand<b>Background/Objective:</b><i>Staphylococcus aureus</i> produces staphyloferrin A (Sfna) siderophores to sequester host iron during infection and rapid cell proliferation We examined drug susceptibility, siderophore production, and genome sequencing of clinical isolates of <i>S. aureus</i>. <b>Methods:</b> A total of 100 specimens, including pus, sputum, hemoculture, urine, tissue, fluid, and skin scrap specimens, were grown in iron-deprived Luria broth agar. The isolates were investigated for spectral signature using MALDI–TOF/MS, while antibiotic susceptibility and siderophore content were assessed using the chrome azurol S method. Whole genome and partial 16S rRNA DNA sequences were employed, and VITEK/MS revealed specific spectra. <b>Results:</b> Clindamycin, erythromycin, gentamicin, linezolid, moxifloxacin, oxacillin, trimethoprim/sulfamethoxazole, and vancomycin (100%) were the most common antibiotics to which the <i>S. aureus</i> isolates were susceptible. Sfna was not detectable in fluid and skin scrap isolates, which were encoded by <i>sfnaB</i>, <i>sfnaD</i>, and <i>sfnaB</i>/<i>sfnaD</i> genes. However, they were detectable in pus (73.8%), sputum (85.3%), hemoculture (50.0%), and urine (85.7%) isolates. The aureus subspecies, JKD6159, SA268, and MN8, were found to be 72.73% according to genome sequencing. <b>Conclusion:</b> most staphylococci in the isolates, including <i>S. aureus</i> JKD6159, SA268, and MN8, were sensitive to antibiotics and were detected by MALDI–TOF/MS, resulting in the production of Sfna encoded by <i>sfna</i> genes.https://www.mdpi.com/2079-6382/14/5/521siderophoreiron<i>Staphylococcus</i>staphyloferringenomeDNA sequencing |
| spellingShingle | Warinda Prommachote Manu Deeudom Pimpisid Koonyosying Phronpawee Srichomphoo Ratchanee Somnabut Phadungkiat Khamnoi Agostino Cilibrizzi Yuvaraj Ravikumar Somdet Srichairatanakool Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand Antibiotics siderophore iron <i>Staphylococcus</i> staphyloferrin genome DNA sequencing |
| title | Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand |
| title_full | Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand |
| title_fullStr | Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand |
| title_full_unstemmed | Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand |
| title_short | Drug Susceptibility, Siderophore Production, and Genome Analysis of <i>Staphylococcus aureus</i> Clinical Isolates from a University Hospital in Chiang Mai, Thailand |
| title_sort | drug susceptibility siderophore production and genome analysis of i staphylococcus aureus i clinical isolates from a university hospital in chiang mai thailand |
| topic | siderophore iron <i>Staphylococcus</i> staphyloferrin genome DNA sequencing |
| url | https://www.mdpi.com/2079-6382/14/5/521 |
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