Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus
Namanereis sp. belongs to class Polychaeta, a group of main benthic community in marine ecosystems. The purpose of this study to determine the potential of bioactive compounds from Namanereis sp. extract as an antibacterial agent and to determine its extract content through Gas Chromatography–Mass S...
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Diponegoro University; Association of Indonesian Coastal Management Experts
2021-09-01
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| Series: | Ilmu Kelautan |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ijms/article/view/35695 |
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| author | Delianis Pringgenies Endah Sari Widianingsih Widianingsih Anuwat Nateewathana |
| author_facet | Delianis Pringgenies Endah Sari Widianingsih Widianingsih Anuwat Nateewathana |
| author_sort | Delianis Pringgenies |
| collection | DOAJ |
| description | Namanereis sp. belongs to class Polychaeta, a group of main benthic community in marine ecosystems. The purpose of this study to determine the potential of bioactive compounds from Namanereis sp. extract as an antibacterial agent and to determine its extract content through Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Samples of Namaneries sp. were collected from Demak Waters, Central of Java. This research used experimental laboratory method. Samples were extracted with non-polar (N-Hexane), semi-polar (Ethyl acetate) and polar (Methanol) solutions. Different concentrations of extraction, i.e. 5, 10, 20, 40, 80, and 100 µg.disk-1. then were tested against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The extract content analysis was obtained using the GC-MS method. The results showed that Namanereis sp. extract had a potential as an antibacterial agent. The highest inhibition zone was seen in the fraction with ethyl acetate solvent at a concentration of 100%, namely 13±3.05 mm in E. coli and 14±3.04 mm in S. aureus, which then was analysed with GC-MS. Its results found 20 bioactive compounds in the extract. The five most dominant compounds determined by the highest peak were Dihydrojasmone (12.31%), Hexanoic acid (11.49%), Cholesterol (7.66%), Cyclohexanol (7.53%) and Isopropyl Palmitate (7.52%). The research concluded that Namanereis sp. possessed antibacterial potency and contained several bioactive compounds. |
| format | Article |
| id | doaj-art-e9ca96f45b9a4561b754cdeb471e2b6c |
| institution | Kabale University |
| issn | 0853-7291 2406-7598 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Diponegoro University; Association of Indonesian Coastal Management Experts |
| record_format | Article |
| series | Ilmu Kelautan |
| spelling | doaj-art-e9ca96f45b9a4561b754cdeb471e2b6c2025-08-20T03:29:14ZengDiponegoro University; Association of Indonesian Coastal Management ExpertsIlmu Kelautan0853-72912406-75982021-09-0126318218810.14710/ik.ijms.26.3.182-18819160Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureusDelianis Pringgenies0Endah Sari1Widianingsih Widianingsih2https://orcid.org/0000-0003-0064-305XAnuwat Nateewathana3Marine Science Study Program, Faculty of Fisheries and Marine Sciences, Diponegoro University, IndonesiaMarine Science Study Program, Faculty of Fisheries and Marine Sciences, Diponegoro University, IndonesiaMarine Science Study Program, Faculty of Fisheries and Marine Sciences, Diponegoro University, IndonesiaDepartement of Marine and Coastal Resources Bangkok, ThailandNamanereis sp. belongs to class Polychaeta, a group of main benthic community in marine ecosystems. The purpose of this study to determine the potential of bioactive compounds from Namanereis sp. extract as an antibacterial agent and to determine its extract content through Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Samples of Namaneries sp. were collected from Demak Waters, Central of Java. This research used experimental laboratory method. Samples were extracted with non-polar (N-Hexane), semi-polar (Ethyl acetate) and polar (Methanol) solutions. Different concentrations of extraction, i.e. 5, 10, 20, 40, 80, and 100 µg.disk-1. then were tested against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The extract content analysis was obtained using the GC-MS method. The results showed that Namanereis sp. extract had a potential as an antibacterial agent. The highest inhibition zone was seen in the fraction with ethyl acetate solvent at a concentration of 100%, namely 13±3.05 mm in E. coli and 14±3.04 mm in S. aureus, which then was analysed with GC-MS. Its results found 20 bioactive compounds in the extract. The five most dominant compounds determined by the highest peak were Dihydrojasmone (12.31%), Hexanoic acid (11.49%), Cholesterol (7.66%), Cyclohexanol (7.53%) and Isopropyl Palmitate (7.52%). The research concluded that Namanereis sp. possessed antibacterial potency and contained several bioactive compounds.https://ejournal.undip.ac.id/index.php/ijms/article/view/35695polychaetanamanereis spantibacterialbioactive compounds |
| spellingShingle | Delianis Pringgenies Endah Sari Widianingsih Widianingsih Anuwat Nateewathana Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus Ilmu Kelautan polychaeta namanereis sp antibacterial bioactive compounds |
| title | Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus |
| title_full | Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus |
| title_fullStr | Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus |
| title_full_unstemmed | Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus |
| title_short | Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus |
| title_sort | exploration of bioactive compounds potency of extract namanereis sp polychaeta annelida as an antibacterial agent against escherichia coli and staphylococcus aureus |
| topic | polychaeta namanereis sp antibacterial bioactive compounds |
| url | https://ejournal.undip.ac.id/index.php/ijms/article/view/35695 |
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