Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources
Lactic acid bacteria (LAB) are known as producers of various antimicrobial compounds. Among these, bacteriocins have attracted considerable interest because of their potential use as natural food preservatives. The aim of this study was to identify potential strains from Egyptian sources that have u...
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2025-02-01
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| Series: | Applied Microbiology |
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| author | Mohamed Abdelfattah Maky Naoki Ishibashi Xiao Gong Kenji Sonomoto Takeshi Zendo |
| author_facet | Mohamed Abdelfattah Maky Naoki Ishibashi Xiao Gong Kenji Sonomoto Takeshi Zendo |
| author_sort | Mohamed Abdelfattah Maky |
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| description | Lactic acid bacteria (LAB) are known as producers of various antimicrobial compounds. Among these, bacteriocins have attracted considerable interest because of their potential use as natural food preservatives. The aim of this study was to identify potential strains from Egyptian sources that have unique antibacterial activity for possible future use. In this current study, 835 LAB strains were isolated from different Egyptian sources such as meat, salted fish, chicken byproducts, dairy products, and a starter for fermentation. The bacteriocin activity of cell-free culture supernatants was tested using the spot-on-lawn method against eight indicator strains. As a result, 237 isolates were found to produce bacteriocin-like substances (BLS). According to their antimicrobial spectra, they were classified into three groups: the broad spectrum group (2.1% of isolates), the middle spectrum group (15.2% of isolates), and the narrow spectrum group (82.7% of isolates). 16S rDNA sequencing showed that all isolates belonged to LAB strains such as <i>Lactobacillus</i>, <i>Enterococcus</i>, <i>Carnobacterium</i>, <i>Weissella</i>, and <i>Leuconostoc</i>. Egyptian materials were found to be promising sources of bacteriocin-producing LAB. The BLS generated from LAB in this current work have a diverse antimicrobial spectrum against numerous bacterial hazards, including <i>Listeria</i>. The new strains identified in this study were shown to have characteristic antimicrobial spectra and can be used in the future as effective preservatives in the food industry. |
| format | Article |
| id | doaj-art-e9c21eee9d374bafac4a349bc162cc75 |
| institution | DOAJ |
| issn | 2673-8007 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-e9c21eee9d374bafac4a349bc162cc752025-08-20T02:42:41ZengMDPI AGApplied Microbiology2673-80072025-02-01512010.3390/applmicrobiol5010020Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian SourcesMohamed Abdelfattah Maky0Naoki Ishibashi1Xiao Gong2Kenji Sonomoto3Takeshi Zendo4Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanLaboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanLaboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanLaboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanLaboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanLactic acid bacteria (LAB) are known as producers of various antimicrobial compounds. Among these, bacteriocins have attracted considerable interest because of their potential use as natural food preservatives. The aim of this study was to identify potential strains from Egyptian sources that have unique antibacterial activity for possible future use. In this current study, 835 LAB strains were isolated from different Egyptian sources such as meat, salted fish, chicken byproducts, dairy products, and a starter for fermentation. The bacteriocin activity of cell-free culture supernatants was tested using the spot-on-lawn method against eight indicator strains. As a result, 237 isolates were found to produce bacteriocin-like substances (BLS). According to their antimicrobial spectra, they were classified into three groups: the broad spectrum group (2.1% of isolates), the middle spectrum group (15.2% of isolates), and the narrow spectrum group (82.7% of isolates). 16S rDNA sequencing showed that all isolates belonged to LAB strains such as <i>Lactobacillus</i>, <i>Enterococcus</i>, <i>Carnobacterium</i>, <i>Weissella</i>, and <i>Leuconostoc</i>. Egyptian materials were found to be promising sources of bacteriocin-producing LAB. The BLS generated from LAB in this current work have a diverse antimicrobial spectrum against numerous bacterial hazards, including <i>Listeria</i>. The new strains identified in this study were shown to have characteristic antimicrobial spectra and can be used in the future as effective preservatives in the food industry.https://www.mdpi.com/2673-8007/5/1/20lactic acid bacteriabacteriocinsantimicrobial peptides |
| spellingShingle | Mohamed Abdelfattah Maky Naoki Ishibashi Xiao Gong Kenji Sonomoto Takeshi Zendo Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources Applied Microbiology lactic acid bacteria bacteriocins antimicrobial peptides |
| title | Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources |
| title_full | Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources |
| title_fullStr | Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources |
| title_full_unstemmed | Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources |
| title_short | Distribution of Bacteriocin-like Substance-Producing Lactic Acid Bacteria in Egyptian Sources |
| title_sort | distribution of bacteriocin like substance producing lactic acid bacteria in egyptian sources |
| topic | lactic acid bacteria bacteriocins antimicrobial peptides |
| url | https://www.mdpi.com/2673-8007/5/1/20 |
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