Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches
This work aims to develop an insecticidal biofilm based on Calothrixin A, collagen, and chitosan for the protection of pea seeds. The main objective is to improve the ingredient concentrations maximizing the insecticidal activity of the biofilm and to study the desorption of Calothrixin A according...
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MDPI AG
2025-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/7/1621 |
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| author | Fatouma Mohamed Abdoul-Latif My Ismail El Mhamdi Ayoub Ainane Ali Merito Ali Khadija Oumaskour Sanaa Cherroud Stefano Cacciatore Tarik Ainane |
| author_facet | Fatouma Mohamed Abdoul-Latif My Ismail El Mhamdi Ayoub Ainane Ali Merito Ali Khadija Oumaskour Sanaa Cherroud Stefano Cacciatore Tarik Ainane |
| author_sort | Fatouma Mohamed Abdoul-Latif |
| collection | DOAJ |
| description | This work aims to develop an insecticidal biofilm based on Calothrixin A, collagen, and chitosan for the protection of pea seeds. The main objective is to improve the ingredient concentrations maximizing the insecticidal activity of the biofilm and to study the desorption of Calothrixin A according to the diffusion parameters. Eight biofilm formulations were prepared with different concentrations of the components and tested on <i>Sitona lineatus</i> and <i>Bruchus pisorum</i>. The results show that a high concentration of Calothrixin A tended to increase insecticidal activity, although this increase was not always significant, while a higher concentration of collagen and chitosan reduced insecticidal activity, probably by limiting the diffusion of the active ingredient. The prediction models for insecticidal activity showed that the interaction of the factors had no significant impact on the responses, but the model for <i>Sitona lineatus</i> presented better accuracy. The diffusion tests revealed that the CB3C-5 biofilm, with high diffusion parameters, correlated with insecticidal activity. The characterization of the CB3C-5 biofilm showed adequate physical, mechanical, thermal, and structural properties for agricultural seed storage application. Moreover, the computational approach showed that Calothrixin A interacts more efficiently with the OR5-Orco complex than with the small OBP, disrupting the olfactory detection of insects. This mechanism highlights the targeting of the olfactory complex as a potential strategy to control insect pests. This research contributes to the understanding of the role of marine-based biofilms for seed protection and opens perspectives for the development of ecological solutions against insect pests, particularly in the field of sustainable agriculture. |
| format | Article |
| id | doaj-art-e9bb41cd00954ec48724835405f62d42 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-e9bb41cd00954ec48724835405f62d422025-08-20T02:09:19ZengMDPI AGMolecules1420-30492025-04-01307162110.3390/molecules30071621Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational ApproachesFatouma Mohamed Abdoul-Latif0My Ismail El Mhamdi1Ayoub Ainane2Ali Merito Ali3Khadija Oumaskour4Sanaa Cherroud5Stefano Cacciatore6Tarik Ainane7Medicinal Research Institute, Centre d’Etudes et de Recherche de Djibouti, IRM-CERD, Route de l’Aéroport, Haramous B.P. 486, Djibouti City 77101, DjiboutiSuperior School of Technology of Khenifra (EST-Khenifra), University of Sultan Moulay Slimane, BP 170, Khenifra 54000, MoroccoSuperior School of Technology of Khenifra (EST-Khenifra), University of Sultan Moulay Slimane, BP 170, Khenifra 54000, MoroccoMedicinal Research Institute, Centre d’Etudes et de Recherche de Djibouti, IRM-CERD, Route de l’Aéroport, Haramous B.P. 486, Djibouti City 77101, DjiboutiSuperior School of Technology of Khenifra (EST-Khenifra), University of Sultan Moulay Slimane, BP 170, Khenifra 54000, MoroccoSuperior School of Technology of Khenifra (EST-Khenifra), University of Sultan Moulay Slimane, BP 170, Khenifra 54000, MoroccoBioinformatics Unit, International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town 7925, South AfricaSuperior School of Technology of Khenifra (EST-Khenifra), University of Sultan Moulay Slimane, BP 170, Khenifra 54000, MoroccoThis work aims to develop an insecticidal biofilm based on Calothrixin A, collagen, and chitosan for the protection of pea seeds. The main objective is to improve the ingredient concentrations maximizing the insecticidal activity of the biofilm and to study the desorption of Calothrixin A according to the diffusion parameters. Eight biofilm formulations were prepared with different concentrations of the components and tested on <i>Sitona lineatus</i> and <i>Bruchus pisorum</i>. The results show that a high concentration of Calothrixin A tended to increase insecticidal activity, although this increase was not always significant, while a higher concentration of collagen and chitosan reduced insecticidal activity, probably by limiting the diffusion of the active ingredient. The prediction models for insecticidal activity showed that the interaction of the factors had no significant impact on the responses, but the model for <i>Sitona lineatus</i> presented better accuracy. The diffusion tests revealed that the CB3C-5 biofilm, with high diffusion parameters, correlated with insecticidal activity. The characterization of the CB3C-5 biofilm showed adequate physical, mechanical, thermal, and structural properties for agricultural seed storage application. Moreover, the computational approach showed that Calothrixin A interacts more efficiently with the OR5-Orco complex than with the small OBP, disrupting the olfactory detection of insects. This mechanism highlights the targeting of the olfactory complex as a potential strategy to control insect pests. This research contributes to the understanding of the role of marine-based biofilms for seed protection and opens perspectives for the development of ecological solutions against insect pests, particularly in the field of sustainable agriculture.https://www.mdpi.com/1420-3049/30/7/1621composite biofilminsecticidemarine productsprotectionpea seedscalothrixin A |
| spellingShingle | Fatouma Mohamed Abdoul-Latif My Ismail El Mhamdi Ayoub Ainane Ali Merito Ali Khadija Oumaskour Sanaa Cherroud Stefano Cacciatore Tarik Ainane Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches Molecules composite biofilm insecticide marine products protection pea seeds calothrixin A |
| title | Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches |
| title_full | Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches |
| title_fullStr | Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches |
| title_full_unstemmed | Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches |
| title_short | Development and Perfection of Marine-Based Insecticide Biofilm for Pea Seed Protection: Experimental and Computational Approaches |
| title_sort | development and perfection of marine based insecticide biofilm for pea seed protection experimental and computational approaches |
| topic | composite biofilm insecticide marine products protection pea seeds calothrixin A |
| url | https://www.mdpi.com/1420-3049/30/7/1621 |
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