Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
Summary: Inflammatory cells infiltration in the cerebrospinal fluid is a hallmark of severe enterovirus 71 (EV71) infection, but which type of immune cells are critical for severe EV71 infection remains unclear. Here, we observe that both neutrophils and macrophages are increased in the brains of pa...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224026130 |
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| author | Wenxian Yang Li Li Guanlin Li Xiuhui Li Hongyan Liu Xuelian Han Yuan Wang Yali Sun Yuwei Wei Bo Gao Guangyu Zhao Lei Sun Min Li |
| author_facet | Wenxian Yang Li Li Guanlin Li Xiuhui Li Hongyan Liu Xuelian Han Yuan Wang Yali Sun Yuwei Wei Bo Gao Guangyu Zhao Lei Sun Min Li |
| author_sort | Wenxian Yang |
| collection | DOAJ |
| description | Summary: Inflammatory cells infiltration in the cerebrospinal fluid is a hallmark of severe enterovirus 71 (EV71) infection, but which type of immune cells are critical for severe EV71 infection remains unclear. Here, we observe that both neutrophils and macrophages are increased in the brains of patients and mice with severe EV71 infection, and the depletion of neutrophils but not macrophages results in a marked enhancement of survival of EV71-infected mice. Furthermore, CCR1/3 may play an important role in CCL3 facilitating the accumulation of neutrophils in the brains of patients. Inhibition of CCL3 by anti-CCL3 antibodies or selected miRNAs significantly reduces the neutrophils infiltration in brains and the mortality of EV71-infected mice. Collectively, CCL3-mediated neutrophils recruitment into the brain contributes to the severe immunopathology of EV71 infection, which provides a potential diagnostic and therapeutic target for EV71 infection. |
| format | Article |
| id | doaj-art-e9b9aa25156d46dfa797d0c4d68f58b4 |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-e9b9aa25156d46dfa797d0c4d68f58b42025-08-20T02:40:14ZengElsevieriScience2589-00422024-12-01271211138810.1016/j.isci.2024.111388Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infectionWenxian Yang0Li Li1Guanlin Li2Xiuhui Li3Hongyan Liu4Xuelian Han5Yuan Wang6Yali Sun7Yuwei Wei8Bo Gao9Guangyu Zhao10Lei Sun11Min Li12CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaYou’an Hospital, Capital Medical University, Beijing, Fengtai 100069, ChinaAssociate Chief Technician, Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Key Clinical Laboratory of Henan Province, Zhengzhou, ChinaYou’an Hospital, Capital Medical University, Beijing, Fengtai 100069, ChinaShenyang Infectious Diseases Hospital, Shenyang, Liaoning Province, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, ChinaBeijing Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China; Corresponding authorState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China; Corresponding authorCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Corresponding authorState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China; Corresponding authorSummary: Inflammatory cells infiltration in the cerebrospinal fluid is a hallmark of severe enterovirus 71 (EV71) infection, but which type of immune cells are critical for severe EV71 infection remains unclear. Here, we observe that both neutrophils and macrophages are increased in the brains of patients and mice with severe EV71 infection, and the depletion of neutrophils but not macrophages results in a marked enhancement of survival of EV71-infected mice. Furthermore, CCR1/3 may play an important role in CCL3 facilitating the accumulation of neutrophils in the brains of patients. Inhibition of CCL3 by anti-CCL3 antibodies or selected miRNAs significantly reduces the neutrophils infiltration in brains and the mortality of EV71-infected mice. Collectively, CCL3-mediated neutrophils recruitment into the brain contributes to the severe immunopathology of EV71 infection, which provides a potential diagnostic and therapeutic target for EV71 infection.http://www.sciencedirect.com/science/article/pii/S2589004224026130ImmunologyClinical neuroscienceVirology |
| spellingShingle | Wenxian Yang Li Li Guanlin Li Xiuhui Li Hongyan Liu Xuelian Han Yuan Wang Yali Sun Yuwei Wei Bo Gao Guangyu Zhao Lei Sun Min Li Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection iScience Immunology Clinical neuroscience Virology |
| title | Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection |
| title_full | Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection |
| title_fullStr | Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection |
| title_full_unstemmed | Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection |
| title_short | Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection |
| title_sort | blocking ccl3 mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection |
| topic | Immunology Clinical neuroscience Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224026130 |
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