Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes
The effect of increasing ageing time (t) of starch on the activity of three amylolytic enzymes (Termamyl, San Super, and BAN) was investigated. Although all the enzymatic reactions follow michaelian kinetics, vmax decreased significantly (P<0.05) and KM increased (although not always significantl...
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/402439 |
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| author | Nelson P. Guerra Lorenzo Pastrana Castro |
| author_facet | Nelson P. Guerra Lorenzo Pastrana Castro |
| author_sort | Nelson P. Guerra |
| collection | DOAJ |
| description | The effect of increasing ageing time (t) of starch on the activity of three amylolytic enzymes (Termamyl, San Super, and BAN) was investigated. Although all the enzymatic reactions follow michaelian kinetics, vmax decreased significantly (P<0.05) and KM increased (although not always significantly) with the increase in t. The conformational changes produced in the starch chains as a consequence of the ageing seemed to affect negatively the diffusivity of the starch to the active site of the enzymes and the release of the reaction products to the medium. A similar effect was observed when the enzymatic reactions were carried out with unaged starches supplemented with different concentrations of gelatine [G]. The inhibition in the amylolytic activities was best mathematically described by using three modified forms of the Michaelis-Menten model, which included a term to consider, respectively, the linear, exponential, and hyperbolic inhibitory effects of t and [G]. |
| format | Article |
| id | doaj-art-e9b10725aaf7482589f2f9c76946205e |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e9b10725aaf7482589f2f9c76946205e2025-08-20T02:21:54ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/402439402439Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic EnzymesNelson P. Guerra0Lorenzo Pastrana Castro1Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo, Ourense Campus, 32004 Ourense, SpainDepartment of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo, Ourense Campus, 32004 Ourense, SpainThe effect of increasing ageing time (t) of starch on the activity of three amylolytic enzymes (Termamyl, San Super, and BAN) was investigated. Although all the enzymatic reactions follow michaelian kinetics, vmax decreased significantly (P<0.05) and KM increased (although not always significantly) with the increase in t. The conformational changes produced in the starch chains as a consequence of the ageing seemed to affect negatively the diffusivity of the starch to the active site of the enzymes and the release of the reaction products to the medium. A similar effect was observed when the enzymatic reactions were carried out with unaged starches supplemented with different concentrations of gelatine [G]. The inhibition in the amylolytic activities was best mathematically described by using three modified forms of the Michaelis-Menten model, which included a term to consider, respectively, the linear, exponential, and hyperbolic inhibitory effects of t and [G].http://dx.doi.org/10.1100/2012/402439 |
| spellingShingle | Nelson P. Guerra Lorenzo Pastrana Castro Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes The Scientific World Journal |
| title | Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes |
| title_full | Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes |
| title_fullStr | Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes |
| title_full_unstemmed | Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes |
| title_short | Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes |
| title_sort | modelling the effects of ageing time of starch on the enzymatic activity of three amylolytic enzymes |
| url | http://dx.doi.org/10.1100/2012/402439 |
| work_keys_str_mv | AT nelsonpguerra modellingtheeffectsofageingtimeofstarchontheenzymaticactivityofthreeamylolyticenzymes AT lorenzopastranacastro modellingtheeffectsofageingtimeofstarchontheenzymaticactivityofthreeamylolyticenzymes |