Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations

Cucumber plants are vulnerable to soil-borne fungal diseases. Fungicides control cucumber diseases but pose health risks from pesticide residues. Native bio agent strains suppressing plant pathogens while promoting plant growth and maintaining soil health.This study aimed to isolate and identify ind...

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Main Authors: Khairy A. Abada, Abdelrhman M. A. El-Nady, Shereen E. M. El-Nahas, Marwa A. Zayton
Format: Article
Language:English
Published: University of Bologna 2025-05-01
Series:Italian Journal of Mycology
Subjects:
Online Access:https://italianmycology.unibo.it/article/view/21066
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author Khairy A. Abada
Abdelrhman M. A. El-Nady
Shereen E. M. El-Nahas
Marwa A. Zayton
author_facet Khairy A. Abada
Abdelrhman M. A. El-Nady
Shereen E. M. El-Nahas
Marwa A. Zayton
author_sort Khairy A. Abada
collection DOAJ
description Cucumber plants are vulnerable to soil-borne fungal diseases. Fungicides control cucumber diseases but pose health risks from pesticide residues. Native bio agent strains suppressing plant pathogens while promoting plant growth and maintaining soil health.This study aimed to isolate and identify indigenous Trichoderma species and evaluate their efficacy as cucumber seed dressings for controlling damping-off caused by Rhizoctonia solani, Fusarium solani, Macrophomina phaseolina, and Sclerotium rolfsii. Trichoderma afroharzianum demonstrated the highest radial growth inhibition (81.3%) against M. phaseolina, while T. asperellum showed 75.0% inhibition against F. solani. Seed dressing with these bio-agents significantly reduced damping-off incidence, increased plant survival, and enhanced the accumulation of phenolic compounds, including total phenols. It also boosted peroxidase, polyphenoloxidase, and carboxymethyl cellulase activities. Furthermore, T. afroharzianum improved chlorophyll and carotenoid levels, promoting photosynthesis and disease resistance. The two Trichoderma species exhibit potential as novel biocontrol agents with antifungal and plant growth-promoting properties.
format Article
id doaj-art-e9b0ee0c24d2467dbc0dd6e3bf7bea86
institution DOAJ
issn 2531-7342
language English
publishDate 2025-05-01
publisher University of Bologna
record_format Article
series Italian Journal of Mycology
spelling doaj-art-e9b0ee0c24d2467dbc0dd6e3bf7bea862025-08-20T03:10:11ZengUniversity of BolognaItalian Journal of Mycology2531-73422025-05-0154779710.6092/issn.2531-7342/2106619440Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterationsKhairy A. Abada0Abdelrhman M. A. El-Nady1Shereen E. M. El-Nahas2Marwa A. Zayton3Department of Plant Pathology, Faculty of Agriculture, Cairo University, Cairo, Giza 12613, EgyptDepartment of Plant Pathology, Faculty of Agriculture, Cairo University, Cairo, Giza 12613, EgyptDepartmemt of Integrated Control Research, Plant Pathology Institute, Agricultural Research Center, Cairo, Giza 12613, EgyptDepartment of Plant Pathology, Faculty of Agriculture, Cairo University, Cairo, Giza 12613, EgyptCucumber plants are vulnerable to soil-borne fungal diseases. Fungicides control cucumber diseases but pose health risks from pesticide residues. Native bio agent strains suppressing plant pathogens while promoting plant growth and maintaining soil health.This study aimed to isolate and identify indigenous Trichoderma species and evaluate their efficacy as cucumber seed dressings for controlling damping-off caused by Rhizoctonia solani, Fusarium solani, Macrophomina phaseolina, and Sclerotium rolfsii. Trichoderma afroharzianum demonstrated the highest radial growth inhibition (81.3%) against M. phaseolina, while T. asperellum showed 75.0% inhibition against F. solani. Seed dressing with these bio-agents significantly reduced damping-off incidence, increased plant survival, and enhanced the accumulation of phenolic compounds, including total phenols. It also boosted peroxidase, polyphenoloxidase, and carboxymethyl cellulase activities. Furthermore, T. afroharzianum improved chlorophyll and carotenoid levels, promoting photosynthesis and disease resistance. The two Trichoderma species exhibit potential as novel biocontrol agents with antifungal and plant growth-promoting properties.https://italianmycology.unibo.it/article/view/21066t. afroharzianumt. asperellumseed dressingdefense-related enzymes
spellingShingle Khairy A. Abada
Abdelrhman M. A. El-Nady
Shereen E. M. El-Nahas
Marwa A. Zayton
Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations
Italian Journal of Mycology
t. afroharzianum
t. asperellum
seed dressing
defense-related enzymes
title Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations
title_full Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations
title_fullStr Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations
title_full_unstemmed Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations
title_short Trichoderma as a biocontrol agent for damping-off and its impact on cucumber biochemical alterations
title_sort trichoderma as a biocontrol agent for damping off and its impact on cucumber biochemical alterations
topic t. afroharzianum
t. asperellum
seed dressing
defense-related enzymes
url https://italianmycology.unibo.it/article/view/21066
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AT abdelrhmanmaelnady trichodermaasabiocontrolagentfordampingoffanditsimpactoncucumberbiochemicalalterations
AT shereenemelnahas trichodermaasabiocontrolagentfordampingoffanditsimpactoncucumberbiochemicalalterations
AT marwaazayton trichodermaasabiocontrolagentfordampingoffanditsimpactoncucumberbiochemicalalterations