The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis
Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. In Arabidopsis and rice, several RLCKs in subfamily VII (RLCKs-VII) have been implicated in pathogen-associated molecular pattern-triggered immunity and basal resistance against bacterial and fungal pathogens....
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311920631604 |
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| author | Tian-ci WU Xiu-liang ZHU Liang-jie LÜ Xi-yong CHEN Gang-biao XU Zeng-yan ZHANG |
| author_facet | Tian-ci WU Xiu-liang ZHU Liang-jie LÜ Xi-yong CHEN Gang-biao XU Zeng-yan ZHANG |
| author_sort | Tian-ci WU |
| collection | DOAJ |
| description | Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. In Arabidopsis and rice, several RLCKs in subfamily VII (RLCKs-VII) have been implicated in pathogen-associated molecular pattern-triggered immunity and basal resistance against bacterial and fungal pathogens. However, little is known about roles of RLCKs-VII of the important crop common wheat (Triticum aestivum) in immune responses. Here, we isolated a RLCK-VII-encoding gene from wheat, designated as TaRLCK1B, and investigated its role in host immune response to infection of a necrotrophic fungus Rhizoctonia cerealis that is a major pathogen of sharp eyespot, a destructive disease of wheat. RNA-sequencing and RT-qPCR analyses showed that transcriptional level of TaRLCK1B was significantly higher in sharp eyespot-resistant wheat cultivars than in susceptible wheat cultivars. The gene transcription was rapidly and markedly elevated in the resistant wheat cultivars by R. cerealis infection. The TaRLCK1B protein was closely related to OsRLCK176, a rice resistance-related RLCKs-VII, with 84.03% identity. Virus-induced gene silencing plus wheat response to R. cerealis assay results indicated that silencing of TaRLCK1 impaired resistance to R. cerealis. Meantime, silencing of TaRLCK1 significantly elevated both the content of H2O2 (a major kind of reactive oxygen species, ROS) and the transcriptional level of the ROS-generating enzyme-encoding gene RBOH, but repressed the expression of the ROS-scavenging enzyme-encoding gene CAT1 at 18 hours after inoculation (hai) with R. cerealis. Taken together, these data suggested that TaRLCK1B was required for the early immune response of wheat to R. cerealis through modulating ROS signaling in wheat. |
| format | Article |
| id | doaj-art-e9a76a3a532048c0af876de5ec903394 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e9a76a3a532048c0af876de5ec9033942025-08-20T03:57:52ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192020-11-0119112616262710.1016/S2095-3119(20)63160-4The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealisTian-ci WU0Xiu-liang ZHU1Liang-jie LÜ2Xi-yong CHEN3Gang-biao XU4Zeng-yan ZHANG5Laboratory of Forestry Genetics, Central South University of Forestry and Technology, Changsha 410004, P.R.China; National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaInstitute for Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.ChinaInstitute for Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.ChinaLaboratory of Forestry Genetics, Central South University of Forestry and Technology, Changsha 410004, P.R.ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China; Correspondence ZHANG Zeng-yan, Tel: +86-10-82108781Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. In Arabidopsis and rice, several RLCKs in subfamily VII (RLCKs-VII) have been implicated in pathogen-associated molecular pattern-triggered immunity and basal resistance against bacterial and fungal pathogens. However, little is known about roles of RLCKs-VII of the important crop common wheat (Triticum aestivum) in immune responses. Here, we isolated a RLCK-VII-encoding gene from wheat, designated as TaRLCK1B, and investigated its role in host immune response to infection of a necrotrophic fungus Rhizoctonia cerealis that is a major pathogen of sharp eyespot, a destructive disease of wheat. RNA-sequencing and RT-qPCR analyses showed that transcriptional level of TaRLCK1B was significantly higher in sharp eyespot-resistant wheat cultivars than in susceptible wheat cultivars. The gene transcription was rapidly and markedly elevated in the resistant wheat cultivars by R. cerealis infection. The TaRLCK1B protein was closely related to OsRLCK176, a rice resistance-related RLCKs-VII, with 84.03% identity. Virus-induced gene silencing plus wheat response to R. cerealis assay results indicated that silencing of TaRLCK1 impaired resistance to R. cerealis. Meantime, silencing of TaRLCK1 significantly elevated both the content of H2O2 (a major kind of reactive oxygen species, ROS) and the transcriptional level of the ROS-generating enzyme-encoding gene RBOH, but repressed the expression of the ROS-scavenging enzyme-encoding gene CAT1 at 18 hours after inoculation (hai) with R. cerealis. Taken together, these data suggested that TaRLCK1B was required for the early immune response of wheat to R. cerealis through modulating ROS signaling in wheat.http://www.sciencedirect.com/science/article/pii/S2095311920631604common wheat (Triticum aestivum)H2O2immune responsereactive oxygen speciesreceptor-like cytoplasmic kinaseRhizoctonia cerealis |
| spellingShingle | Tian-ci WU Xiu-liang ZHU Liang-jie LÜ Xi-yong CHEN Gang-biao XU Zeng-yan ZHANG The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis Journal of Integrative Agriculture common wheat (Triticum aestivum) H2O2 immune response reactive oxygen species receptor-like cytoplasmic kinase Rhizoctonia cerealis |
| title | The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis |
| title_full | The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis |
| title_fullStr | The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis |
| title_full_unstemmed | The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis |
| title_short | The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis |
| title_sort | wheat receptor like cytoplasmic kinase tarlck1b is required for host immune response to the necrotrophic pathogen rhizoctonia cerealis |
| topic | common wheat (Triticum aestivum) H2O2 immune response reactive oxygen species receptor-like cytoplasmic kinase Rhizoctonia cerealis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311920631604 |
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