Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model
Alveolar bone loss due to trauma, extraction, or periodontal disease often requires bone grafting prior to implant placement. Although human allograft bone is widely used as an alternative to autograft, it has limited osteoinductive potential and a prolonged healing time. Mesenchymal stem cell–condi...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/16/7/251 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849733923281567744 |
|---|---|
| author | Hyung-Gyun Kim Yong-Suk Moon Dong-Seok Sohn |
| author_facet | Hyung-Gyun Kim Yong-Suk Moon Dong-Seok Sohn |
| author_sort | Hyung-Gyun Kim |
| collection | DOAJ |
| description | Alveolar bone loss due to trauma, extraction, or periodontal disease often requires bone grafting prior to implant placement. Although human allograft bone is widely used as an alternative to autograft, it has limited osteoinductive potential and a prolonged healing time. Mesenchymal stem cell–conditioned media (MSC-CM), rich in paracrine factors, has emerged as a promising adjunct to enhance bone regeneration. This study evaluated the regenerative effect of MSC-CM combined with human allograft bone in a rabbit calvarial defect model. Bilateral 8 mm defects were created in eight rabbits. Each animal received a human allograft alone (HB group) on one side and an allograft mixed with MSC-CM (HB+GF group) on the other. Histological and histomorphometric analyses were performed at 2 and 8 weeks postoperatively. Both groups showed new bone formation, but the HB+GF group demonstrated significantly greater bone regeneration at both time points (<i>p</i> < 0.05). New bone extended into the defect center in the HB+GF group. Additionally, greater graft resorption and marrow formation were observed in this group at 8 weeks. These findings suggest that MSC-CM enhances the osteogenic performance of human allograft bone and may serve as a biologically active adjunct for bone regeneration. |
| format | Article |
| id | doaj-art-e99701cba6404ed4bc37e61262aa76ea |
| institution | DOAJ |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-e99701cba6404ed4bc37e61262aa76ea2025-08-20T03:07:55ZengMDPI AGJournal of Functional Biomaterials2079-49832025-07-0116725110.3390/jfb16070251Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect ModelHyung-Gyun Kim0Yong-Suk Moon1Dong-Seok Sohn2Department of Dentistry and Advanced General Dentistry, Daegu Catholic University School of Medicine, Daegu 42472, Republic of KoreaDepartment of Anatomy, Daegu Catholic University School of Medicine, Daegu 42472, Republic of KoreaDepartment of Dentistry and Oral and Maxillofacial Surgery, Daegu Catholic University School of Medicine, Daegu 42472, Republic of KoreaAlveolar bone loss due to trauma, extraction, or periodontal disease often requires bone grafting prior to implant placement. Although human allograft bone is widely used as an alternative to autograft, it has limited osteoinductive potential and a prolonged healing time. Mesenchymal stem cell–conditioned media (MSC-CM), rich in paracrine factors, has emerged as a promising adjunct to enhance bone regeneration. This study evaluated the regenerative effect of MSC-CM combined with human allograft bone in a rabbit calvarial defect model. Bilateral 8 mm defects were created in eight rabbits. Each animal received a human allograft alone (HB group) on one side and an allograft mixed with MSC-CM (HB+GF group) on the other. Histological and histomorphometric analyses were performed at 2 and 8 weeks postoperatively. Both groups showed new bone formation, but the HB+GF group demonstrated significantly greater bone regeneration at both time points (<i>p</i> < 0.05). New bone extended into the defect center in the HB+GF group. Additionally, greater graft resorption and marrow formation were observed in this group at 8 weeks. These findings suggest that MSC-CM enhances the osteogenic performance of human allograft bone and may serve as a biologically active adjunct for bone regeneration.https://www.mdpi.com/2079-4983/16/7/251bone regenerationmesenchymal stem cell–conditioned mediahuman allograft bone |
| spellingShingle | Hyung-Gyun Kim Yong-Suk Moon Dong-Seok Sohn Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model Journal of Functional Biomaterials bone regeneration mesenchymal stem cell–conditioned media human allograft bone |
| title | Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model |
| title_full | Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model |
| title_fullStr | Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model |
| title_full_unstemmed | Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model |
| title_short | Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell–Conditioned Media in a Rabbit Calvarial Defect Model |
| title_sort | histologic and histomorphometric evaluation of bone regeneration using human allogeneic bone graft with or without mesenchymal stem cell conditioned media in a rabbit calvarial defect model |
| topic | bone regeneration mesenchymal stem cell–conditioned media human allograft bone |
| url | https://www.mdpi.com/2079-4983/16/7/251 |
| work_keys_str_mv | AT hyunggyunkim histologicandhistomorphometricevaluationofboneregenerationusinghumanallogeneicbonegraftwithorwithoutmesenchymalstemcellconditionedmediainarabbitcalvarialdefectmodel AT yongsukmoon histologicandhistomorphometricevaluationofboneregenerationusinghumanallogeneicbonegraftwithorwithoutmesenchymalstemcellconditionedmediainarabbitcalvarialdefectmodel AT dongseoksohn histologicandhistomorphometricevaluationofboneregenerationusinghumanallogeneicbonegraftwithorwithoutmesenchymalstemcellconditionedmediainarabbitcalvarialdefectmodel |