Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy
<b>Background/Objectives</b>: This study explores the generation of singlet oxygen (SO) through methylene blue (MB) activation as a metabolic intervention for ovarian cancer. We aimed to examine the role of SO in modulating mitochondrial function, cellular metabolism, and proliferation i...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/14/12/648 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850241754722205696 |
|---|---|
| author | Jorgelindo da Veiga Moreira Laurent Schwartz Mario Jolicoeur |
| author_facet | Jorgelindo da Veiga Moreira Laurent Schwartz Mario Jolicoeur |
| author_sort | Jorgelindo da Veiga Moreira |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: This study explores the generation of singlet oxygen (SO) through methylene blue (MB) activation as a metabolic intervention for ovarian cancer. We aimed to examine the role of SO in modulating mitochondrial function, cellular metabolism, and proliferation in ovarian cancer cell lines compared to control cells. <b>Methods</b>: The study utilized two ovarian cancer cell lines, OV1369-R2 and TOV1369, along with ARPE-19 control cells. Following MB treatment and light activation, mitochondrial function and ATP synthesis were assessed. Metabolomic analyses were performed to evaluate changes in central carbon metabolism, particularly focusing on markers of the Warburg effect. <b>Results</b>: TOV1369 cells exhibited a pronounced sensitivity to MB treatment, resulting in significant inhibition of ATP synthesis and reduced proliferation. Metabolomic analysis indicated that MB-induced SO production partially reversed the Warburg effect, suggesting a shift from glycolysis to oxidative phosphorylation. These effects were less pronounced in OV1369-R2 and ARPE-19 cells, correlating with their lower MB sensitivity. <b>Conclusions</b>: MB-generated SO selectively modulates mitochondrial energetics in ovarian cancer cells, driving a metabolic reorganization that curtails their proliferative capacity. This approach, leveraging the bacterial-like features of cancer metabolism, offers a promising therapeutic avenue to induce apoptosis and enhance treatment outcomes in ovarian cancer. |
| format | Article |
| id | doaj-art-e9935231eafd444db81140657d80f737 |
| institution | OA Journals |
| issn | 2218-1989 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-e9935231eafd444db81140657d80f7372025-08-20T02:00:29ZengMDPI AGMetabolites2218-19892024-11-01141264810.3390/metabo14120648Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer TherapyJorgelindo da Veiga Moreira0Laurent Schwartz1Mario Jolicoeur2Research Laboratory in Applied Metabolic Engineering, Department of Chemical Engineering, Polytechnique Montréal, Centre-Ville Station, P.O. Box 6079, Montréal, QC H3C 3A7, CanadaAssistance Publique des Hôpitaux de Paris, Avenue Victoria, 75003 Paris, FranceResearch Laboratory in Applied Metabolic Engineering, Department of Chemical Engineering, Polytechnique Montréal, Centre-Ville Station, P.O. Box 6079, Montréal, QC H3C 3A7, Canada<b>Background/Objectives</b>: This study explores the generation of singlet oxygen (SO) through methylene blue (MB) activation as a metabolic intervention for ovarian cancer. We aimed to examine the role of SO in modulating mitochondrial function, cellular metabolism, and proliferation in ovarian cancer cell lines compared to control cells. <b>Methods</b>: The study utilized two ovarian cancer cell lines, OV1369-R2 and TOV1369, along with ARPE-19 control cells. Following MB treatment and light activation, mitochondrial function and ATP synthesis were assessed. Metabolomic analyses were performed to evaluate changes in central carbon metabolism, particularly focusing on markers of the Warburg effect. <b>Results</b>: TOV1369 cells exhibited a pronounced sensitivity to MB treatment, resulting in significant inhibition of ATP synthesis and reduced proliferation. Metabolomic analysis indicated that MB-induced SO production partially reversed the Warburg effect, suggesting a shift from glycolysis to oxidative phosphorylation. These effects were less pronounced in OV1369-R2 and ARPE-19 cells, correlating with their lower MB sensitivity. <b>Conclusions</b>: MB-generated SO selectively modulates mitochondrial energetics in ovarian cancer cells, driving a metabolic reorganization that curtails their proliferative capacity. This approach, leveraging the bacterial-like features of cancer metabolism, offers a promising therapeutic avenue to induce apoptosis and enhance treatment outcomes in ovarian cancer.https://www.mdpi.com/2218-1989/14/12/648singlet oxygenovarian cancermethylene bluemitochondrial involutionmetabolic bistability |
| spellingShingle | Jorgelindo da Veiga Moreira Laurent Schwartz Mario Jolicoeur Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy Metabolites singlet oxygen ovarian cancer methylene blue mitochondrial involution metabolic bistability |
| title | Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy |
| title_full | Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy |
| title_fullStr | Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy |
| title_full_unstemmed | Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy |
| title_short | Singlet Oxygen-Induced Mitochondrial Reset in Cancer: A Novel Approach for Ovarian Cancer Therapy |
| title_sort | singlet oxygen induced mitochondrial reset in cancer a novel approach for ovarian cancer therapy |
| topic | singlet oxygen ovarian cancer methylene blue mitochondrial involution metabolic bistability |
| url | https://www.mdpi.com/2218-1989/14/12/648 |
| work_keys_str_mv | AT jorgelindodaveigamoreira singletoxygeninducedmitochondrialresetincanceranovelapproachforovariancancertherapy AT laurentschwartz singletoxygeninducedmitochondrialresetincanceranovelapproachforovariancancertherapy AT mariojolicoeur singletoxygeninducedmitochondrialresetincanceranovelapproachforovariancancertherapy |