Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance
Current research in bone tissue engineering is focused not only on basic parameters of the materials, such as biocompatibility and degradation rate but also on intrinsic osteogenic and antimicrobial properties, essential to provide a rapid tissue regeneration without negative effects due to peripros...
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2025-03-01
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| author | Daniil O. Golubchikov Inna V. Fadeeva Alexander V. Knot’ko Iliya A. Kostykov Tatiana K. Slonskaya Katia Barbaro Alessia Zepparoni Marco Fosca Iulian V. Antoniac Julietta V. Rau |
| author_facet | Daniil O. Golubchikov Inna V. Fadeeva Alexander V. Knot’ko Iliya A. Kostykov Tatiana K. Slonskaya Katia Barbaro Alessia Zepparoni Marco Fosca Iulian V. Antoniac Julietta V. Rau |
| author_sort | Daniil O. Golubchikov |
| collection | DOAJ |
| description | Current research in bone tissue engineering is focused not only on basic parameters of the materials, such as biocompatibility and degradation rate but also on intrinsic osteogenic and antimicrobial properties, essential to provide a rapid tissue regeneration without negative effects due to periprosthetic infections, that may result in revision surgeries. One of the major strategies to enhance the osteogenic and antimicrobial performance of calcium phosphates is the ionic substitution, in particular, with magnesium and borates. In this study, we focused on the synthesis of boron-substituted tricalcium phosphate (B-TCP) with a target of 5 mol.% substitution via the solid-state synthesis with mechano-activation. Synthesis from raw precursors, without the preliminary brushite wet precipitation, led to the primary phase of β-TCP, which was proved by the XRD analysis. According to the IR-spectroscopy and <sup>31</sup>P NMR analysis, boron substitution occurred in the synthesized sample. The developed material showed a modest antibacterial performance against <i>E. coli</i>, with 13.5 ± 5.0% growth inhibition, and <i>E. faecalis</i>, with 16.7 ± 5.5% inhibition. The biocompatibility of β-TCP and B-TCP was tested through the MTT assay and osteogenic differentiation of the mesenchymal stromal cells. The proposed synthesis approach can be useful for the fabrication of B-TCP ceramics for bone tissue engineering. |
| format | Article |
| id | doaj-art-e9928c833d464c40a7382f23d30af923 |
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| issn | 1420-3049 |
| language | English |
| publishDate | 2025-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj-art-e9928c833d464c40a7382f23d30af9232025-08-20T02:09:17ZengMDPI AGMolecules1420-30492025-03-01307157510.3390/molecules30071575Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial PerformanceDaniil O. Golubchikov0Inna V. Fadeeva1Alexander V. Knot’ko2Iliya A. Kostykov3Tatiana K. Slonskaya4Katia Barbaro5Alessia Zepparoni6Marco Fosca7Iulian V. Antoniac8Julietta V. Rau9Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaA.A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Leninsky 49, 119334 Moscow, RussiaChemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaChemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaDepartment of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, build. 2, 119048 Moscow, RussiaIstituto Zooprofilattico Sperimentale Lazio e Toscana “M. Aleandri”, Via Appia Nuova 14111, 00178 Rome, ItalyIstituto Zooprofilattico Sperimentale Lazio e Toscana “M. Aleandri”, Via Appia Nuova 14111, 00178 Rome, ItalyIstituto di Struttura della Materia, Consiglio Nazionale delle Ricerche, ISM-CNR, Via del Fosso del Cavaliere 100, 00133 Rome, ItalyFaculty of Material Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, District 6, RO-060042 Bucharest, RomaniaDepartment of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, build. 2, 119048 Moscow, RussiaCurrent research in bone tissue engineering is focused not only on basic parameters of the materials, such as biocompatibility and degradation rate but also on intrinsic osteogenic and antimicrobial properties, essential to provide a rapid tissue regeneration without negative effects due to periprosthetic infections, that may result in revision surgeries. One of the major strategies to enhance the osteogenic and antimicrobial performance of calcium phosphates is the ionic substitution, in particular, with magnesium and borates. In this study, we focused on the synthesis of boron-substituted tricalcium phosphate (B-TCP) with a target of 5 mol.% substitution via the solid-state synthesis with mechano-activation. Synthesis from raw precursors, without the preliminary brushite wet precipitation, led to the primary phase of β-TCP, which was proved by the XRD analysis. According to the IR-spectroscopy and <sup>31</sup>P NMR analysis, boron substitution occurred in the synthesized sample. The developed material showed a modest antibacterial performance against <i>E. coli</i>, with 13.5 ± 5.0% growth inhibition, and <i>E. faecalis</i>, with 16.7 ± 5.5% inhibition. The biocompatibility of β-TCP and B-TCP was tested through the MTT assay and osteogenic differentiation of the mesenchymal stromal cells. The proposed synthesis approach can be useful for the fabrication of B-TCP ceramics for bone tissue engineering.https://www.mdpi.com/1420-3049/30/7/1575bone tissue engineeringtricalcium phosphate ceramicsborate-substituted tricalcium phosphateantimicrobial propertiesbiocompatibility |
| spellingShingle | Daniil O. Golubchikov Inna V. Fadeeva Alexander V. Knot’ko Iliya A. Kostykov Tatiana K. Slonskaya Katia Barbaro Alessia Zepparoni Marco Fosca Iulian V. Antoniac Julietta V. Rau Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance Molecules bone tissue engineering tricalcium phosphate ceramics borate-substituted tricalcium phosphate antimicrobial properties biocompatibility |
| title | Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance |
| title_full | Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance |
| title_fullStr | Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance |
| title_full_unstemmed | Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance |
| title_short | Mechanochemically-Activated Solid-State Synthesis of Borate-Substituted Tricalcium Phosphate: Evaluation of Biocompatibility and Antimicrobial Performance |
| title_sort | mechanochemically activated solid state synthesis of borate substituted tricalcium phosphate evaluation of biocompatibility and antimicrobial performance |
| topic | bone tissue engineering tricalcium phosphate ceramics borate-substituted tricalcium phosphate antimicrobial properties biocompatibility |
| url | https://www.mdpi.com/1420-3049/30/7/1575 |
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