Subcellular localization and expression analysis of MlSOC1 genes during flower bud differentiation period in Magnolia liliflora
In order to clarify the mechanism of SOC1 in the process of flower formation in Magnolia liliflora, two SOC1 genes were screened out based on the transcriptome data in M. sinostellata, and the complete coding regions of these two SOC1 genes were obtained by homologous gene cloning. The bioinformatic...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2020-08-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2019.09.291 |
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| Summary: | In order to clarify the mechanism of SOC1 in the process of flower formation in Magnolia liliflora, two SOC1 genes were screened out based on the transcriptome data in M. sinostellata, and the complete coding regions of these two SOC1 genes were obtained by homologous gene cloning. The bioinformatics software was used to analyze the information of these two genes, and the expression patterns in different flower bud differentiation periods were verified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The results showed that the length of MlSOC1-1 was 666 bp, which encoding 221 amino acids, and the length of MlSOC1-2 was 654 bp, which encoding 217 amino acids. Both of these two MlSOC1 genes contained highly conservative SOC1 motifs, which indicated that the genes belong to the SOC1/TM3 subfamily. Phylogenetic tree analysis showed that MlSOC1-1 was closely related to SOC1 homologous gene of M. praecocissima, while MlSOC1-2 was closely related to SOC1 homologous gene of M. virginiana. Subcellular localization experiments revealed that both genes were located on the nucleus. Besides, the results of expression analysis showed that compared with MlSOC1-2, in addition to participating in the flower transformation of the flower bud in M. liliflora, MlSOC1-1 might also play a role in the flower opening and the synthesis of flower organs. These results show that there are differences in the roles of these two MlSOC1 genes in flower bud differentiation, which provides a theoretical basis for further study of the role of MlSOC1 genes in flower formation of M. liliflora. |
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| ISSN: | 1008-9209 2097-5155 |