Gene-specific monitoring of T7-based RNA amplification by real-time quantitative PCR
T7-based RNA amplification is applied when there is insufficient RNA. The overall extent of amplification can be measured spectrophotometrically (i.e., quantifying RNA yields), but this measurement does not provide information about the RNA amplification of individual genes. Here we describe a metho...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03353st03 |
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