Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017

Introduction: In 2013, the National Tuberculosis (TB) Program of Armenia introduced GeneXpert MTB/RIF (Xpert) assay to address World Health Organization (WHO) target of 80% (2020) of notified new and relapse TB cases to be tested with WHO recommended rapid diagnostic methods. This study aimed to as...

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Main Authors: Eduard Kabasakalyan, Karapet Davtyan, Viktorya Cholakyans, Alvard Mirzoyan, Karine Kentenyants, Diana Petrosyan, Armen Hayrapetyan, Himanshu A Gupte
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2019-05-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/10920
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author Eduard Kabasakalyan
Karapet Davtyan
Viktorya Cholakyans
Alvard Mirzoyan
Karine Kentenyants
Diana Petrosyan
Armen Hayrapetyan
Himanshu A Gupte
author_facet Eduard Kabasakalyan
Karapet Davtyan
Viktorya Cholakyans
Alvard Mirzoyan
Karine Kentenyants
Diana Petrosyan
Armen Hayrapetyan
Himanshu A Gupte
author_sort Eduard Kabasakalyan
collection DOAJ
description Introduction: In 2013, the National Tuberculosis (TB) Program of Armenia introduced GeneXpert MTB/RIF (Xpert) assay to address World Health Organization (WHO) target of 80% (2020) of notified new and relapse TB cases to be tested with WHO recommended rapid diagnostic methods. This study aimed to assess the change in laboratory diagnostic profile of Mycobacterium tuberculosis after introduction of the Xpert assay from 2013 to 2017. Methodology: Retrospective cohort analysis of all presumptive TB patients’ records retrieved from the National Reference Laboratory database was performed. Results: This study showed increased trend of Xpert coverage for suspected TB cases from 25% in 2013 to 86% in 2017 which is in line with WHO TB global strategy’s target of 80% in 2020. In 4.7% cases, Xpert tested positive while microscopy showed negative results. There was also an improved detection of Rifampicin resistance with increased concordance from 99.1% to 99.4% and decreased discordance from 6.7% to 1.4% between culture and Xpert results. Conclusion: Armenia has achieved the 2020 target; in terms of utilizing the GeneXpert it is on track to achieve the End TB strategy target of 100% by 2025. The next step of this research will be assessment of the impact of GeneXpert and other TB tests utilization on the treatment outcomes in Armenia.
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spelling doaj-art-e98a490abde8403cb60dd98f6d6f85932025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-05-011305.110.3855/jidc.10920Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017Eduard Kabasakalyan0Karapet Davtyan1Viktorya Cholakyans2Alvard Mirzoyan3Karine Kentenyants4Diana Petrosyan5Armen Hayrapetyan6Himanshu A Gupte7National Tuberculosis Control Center, SNPO, MoH, ArmeniaFMD K and L Europe Contract Research Organization, Yerevan, ArmeniaNational Tuberculosis Control Center, SNPO, MoH, ArmeniaNational Tuberculosis Control Center, SNPO, MoH, ArmeniaFMD K and L Europe Contract Research Organization, Yerevan, ArmeniaUC Davis Health, Alzheimer ’s disease Center, Walnut Creek, California, United StatesNational Tuberculosis Control Center, SNPO, MoH, ArmeniaNarotam Sekhsaria Foundation, Mumbai, India Introduction: In 2013, the National Tuberculosis (TB) Program of Armenia introduced GeneXpert MTB/RIF (Xpert) assay to address World Health Organization (WHO) target of 80% (2020) of notified new and relapse TB cases to be tested with WHO recommended rapid diagnostic methods. This study aimed to assess the change in laboratory diagnostic profile of Mycobacterium tuberculosis after introduction of the Xpert assay from 2013 to 2017. Methodology: Retrospective cohort analysis of all presumptive TB patients’ records retrieved from the National Reference Laboratory database was performed. Results: This study showed increased trend of Xpert coverage for suspected TB cases from 25% in 2013 to 86% in 2017 which is in line with WHO TB global strategy’s target of 80% in 2020. In 4.7% cases, Xpert tested positive while microscopy showed negative results. There was also an improved detection of Rifampicin resistance with increased concordance from 99.1% to 99.4% and decreased discordance from 6.7% to 1.4% between culture and Xpert results. Conclusion: Armenia has achieved the 2020 target; in terms of utilizing the GeneXpert it is on track to achieve the End TB strategy target of 100% by 2025. The next step of this research will be assessment of the impact of GeneXpert and other TB tests utilization on the treatment outcomes in Armenia. https://jidc.org/index.php/journal/article/view/10920TuberculosisGeneXpert MTB/RIFDiagnosisNRLLaboratory
spellingShingle Eduard Kabasakalyan
Karapet Davtyan
Viktorya Cholakyans
Alvard Mirzoyan
Karine Kentenyants
Diana Petrosyan
Armen Hayrapetyan
Himanshu A Gupte
Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017
Journal of Infection in Developing Countries
Tuberculosis
GeneXpert MTB/RIF
Diagnosis
NRL
Laboratory
title Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017
title_full Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017
title_fullStr Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017
title_full_unstemmed Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017
title_short Change in TB diagnostic profile after introduction of GeneXpert MTB/RIF assay in National TB Program of Armenia, 2013-2017
title_sort change in tb diagnostic profile after introduction of genexpert mtb rif assay in national tb program of armenia 2013 2017
topic Tuberculosis
GeneXpert MTB/RIF
Diagnosis
NRL
Laboratory
url https://jidc.org/index.php/journal/article/view/10920
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