The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation

Perfluorooctanesulfonic acid (PFOS) poses significant health risks from bioaccumulation in humans. Effective strategies for removing bioaccumulated PFOS are limited. This study systematically investigated the association between PFOS reduction and cruciferous vegetable intake through a cross-section...

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Main Authors: Xiaobing Zhai, Junjun Ji, Jing Wang, Jinji Bai, Xuechen Zhao, Wenjie Zhang, Ruyan Lv, Xuan Deng, Qixiu Li, Weiyu Meng, Qianqian Zhang, Jinyuan Zhao, Jiajing Cui, Li Yu, Chi Kin Lam, Chan Zhang, Henry H.Y. Tong, Yuefei Wang, Xicheng Song, Kaiqiang Wang, Junfeng Li, Kefeng Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-01-01
Series:Environmental Chemistry and Ecotoxicology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590182625000980
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author Xiaobing Zhai
Junjun Ji
Jing Wang
Jinji Bai
Xuechen Zhao
Wenjie Zhang
Ruyan Lv
Xuan Deng
Qixiu Li
Weiyu Meng
Qianqian Zhang
Jinyuan Zhao
Jiajing Cui
Li Yu
Chi Kin Lam
Chan Zhang
Henry H.Y. Tong
Yuefei Wang
Xicheng Song
Kaiqiang Wang
Junfeng Li
Kefeng Li
author_facet Xiaobing Zhai
Junjun Ji
Jing Wang
Jinji Bai
Xuechen Zhao
Wenjie Zhang
Ruyan Lv
Xuan Deng
Qixiu Li
Weiyu Meng
Qianqian Zhang
Jinyuan Zhao
Jiajing Cui
Li Yu
Chi Kin Lam
Chan Zhang
Henry H.Y. Tong
Yuefei Wang
Xicheng Song
Kaiqiang Wang
Junfeng Li
Kefeng Li
author_sort Xiaobing Zhai
collection DOAJ
description Perfluorooctanesulfonic acid (PFOS) poses significant health risks from bioaccumulation in humans. Effective strategies for removing bioaccumulated PFOS are limited. This study systematically investigated the association between PFOS reduction and cruciferous vegetable intake through a cross-section analysis of 2258 NHANES participants, followed by a 14-day randomized clinical trial (RCT) validation (n = 38). Plasma lipidomics and reverse docking explored the metabolic changes and potential targets related to PFOS elimination from broccoli intake. In NHANES, frequent cruciferous intake was associated with lower odds of high serum PFOS (OR and 95 % CI: 0.63 [0.41–0.96] after adjustment of all known covariates). The RCT demonstrated a significant decrease in serum PFOS after broccoli-orange juice intervention (median reduction = 5.9 %, P = 0.003), but not after orange juice alone (median reduction = 0.3 %, P = 0.153). Lipdiomics revealed PFOS-reduction related changes in steroid, ganglioside, ceramide metabolism and the urea cycle. Target fishing identified strong binding between the pregnane X receptor (PXR), a regulator of steroid/xenobiotic metabolism, and broccoli bioactives including glucoraphanin and sulforaphane. Overall, these findings have significant implications for developing dietary strategies to mitigate PFOS health risks.
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spelling doaj-art-e9868e1aec374bd483c7d6cba08d18762025-08-20T04:02:09ZengKeAi Communications Co., Ltd.Environmental Chemistry and Ecotoxicology2590-18262025-01-0171648165810.1016/j.enceco.2025.07.003The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validationXiaobing Zhai0Junjun Ji1Jing Wang2Jinji Bai3Xuechen Zhao4Wenjie Zhang5Ruyan Lv6Xuan Deng7Qixiu Li8Weiyu Meng9Qianqian Zhang10Jinyuan Zhao11Jiajing Cui12Li Yu13Chi Kin Lam14Chan Zhang15Henry H.Y. Tong16Yuefei Wang17Xicheng Song18Kaiqiang Wang19Junfeng Li20Kefeng Li21Faculty of Applied Sciences, Macao Polytechnic University, Macao, ChinaFaculty of Applied Sciences, Macao Polytechnic University, Macao, China; Department of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, China; Changzhi Key Lab of Functional Imaging for Brain Diseases, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaDepartment of Critical Care Medicine, Yantai Yuhuangding Hospital, Qingdao University, Shandong, ChinaDepartment of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaSchool of Life Sciences, Shandong University, Shandong, ChinaDepartment of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaDepartment of Critical Care Medicine, Yantai Yuhuangding Hospital, Qingdao University, Shandong, ChinaDepartment of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaDepartment of Critical Care Medicine, Yantai Yuhuangding Hospital, Qingdao University, Shandong, ChinaFaculty of Applied Sciences, Macao Polytechnic University, Macao, ChinaFaculty of Applied Sciences, Macao Polytechnic University, Macao, ChinaDepartment of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaDepartment of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaDepartment of Oncology, Shengjing Hospital of China Medical University, Shenyang, ChinaFaculty of Applied Sciences, Macao Polytechnic University, Macao, ChinaDepartment of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, ChinaFaculty of Applied Sciences, Macao Polytechnic University, Macao, ChinaNational Key Laboratory of Chinese Medicine Modernization, State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Otolaryngology Head and Neck Surgery, Yantai Yuhuangding Hospital, Qingdao University, Shandong, China; Corresponding authors.Shanxi Provincial Department-Municipal Key Laboratory Cultivation Base for Quality Enhancement and Utilization of Shangdang Chinese Medicinal Materials, School of Pharmacy, Changzhi Medical College, Shanxi, China; Changzhi Key Laboratory of Drug Molecular Design and Innovative Pharmaceutics, School of Pharmacy, Changzhi Medical College, Shanxi, China; Correspondence to: Kaiqiang Wang, School of Pharmacy, Changzhi Medical College, Shanxi, China.Department of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, China; Changzhi Key Lab of Functional Imaging for Brain Diseases, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, China; Correspondence to: Junfeng Li, Department of Radiology, Heping Hospital Affiliated to Changzhi Medical College, Shanxi, China.Faculty of Applied Sciences, Macao Polytechnic University, Macao, China; Corresponding authors.Perfluorooctanesulfonic acid (PFOS) poses significant health risks from bioaccumulation in humans. Effective strategies for removing bioaccumulated PFOS are limited. This study systematically investigated the association between PFOS reduction and cruciferous vegetable intake through a cross-section analysis of 2258 NHANES participants, followed by a 14-day randomized clinical trial (RCT) validation (n = 38). Plasma lipidomics and reverse docking explored the metabolic changes and potential targets related to PFOS elimination from broccoli intake. In NHANES, frequent cruciferous intake was associated with lower odds of high serum PFOS (OR and 95 % CI: 0.63 [0.41–0.96] after adjustment of all known covariates). The RCT demonstrated a significant decrease in serum PFOS after broccoli-orange juice intervention (median reduction = 5.9 %, P = 0.003), but not after orange juice alone (median reduction = 0.3 %, P = 0.153). Lipdiomics revealed PFOS-reduction related changes in steroid, ganglioside, ceramide metabolism and the urea cycle. Target fishing identified strong binding between the pregnane X receptor (PXR), a regulator of steroid/xenobiotic metabolism, and broccoli bioactives including glucoraphanin and sulforaphane. Overall, these findings have significant implications for developing dietary strategies to mitigate PFOS health risks.http://www.sciencedirect.com/science/article/pii/S2590182625000980Cruciferous vegetable consumptionPerfluorooctanesulfonic acidRandomized clinical trialCross-sectional designLipidomics
spellingShingle Xiaobing Zhai
Junjun Ji
Jing Wang
Jinji Bai
Xuechen Zhao
Wenjie Zhang
Ruyan Lv
Xuan Deng
Qixiu Li
Weiyu Meng
Qianqian Zhang
Jinyuan Zhao
Jiajing Cui
Li Yu
Chi Kin Lam
Chan Zhang
Henry H.Y. Tong
Yuefei Wang
Xicheng Song
Kaiqiang Wang
Junfeng Li
Kefeng Li
The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation
Environmental Chemistry and Ecotoxicology
Cruciferous vegetable consumption
Perfluorooctanesulfonic acid
Randomized clinical trial
Cross-sectional design
Lipidomics
title The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation
title_full The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation
title_fullStr The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation
title_full_unstemmed The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation
title_short The association between cruciferous vegetable consumption and Perfluorooctanesulfonic acid bioaccumulation: A cross-sectional analysis of the US population and cross-country randomized clinical trial validation
title_sort association between cruciferous vegetable consumption and perfluorooctanesulfonic acid bioaccumulation a cross sectional analysis of the us population and cross country randomized clinical trial validation
topic Cruciferous vegetable consumption
Perfluorooctanesulfonic acid
Randomized clinical trial
Cross-sectional design
Lipidomics
url http://www.sciencedirect.com/science/article/pii/S2590182625000980
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