Anti-inflammatory effect of amalgam on periapical lesion cells in culture
Background/Aim. Amalgam has been used for years in dentistry, but the controversy on its adverse effects, both on local oral/dental tissues and systemic health, still exists. When used for retrograde filling in apical surgery, amalgam comes in close contact with the periapical tissue, and it is some...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2021-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900043E.pdf |
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| author | Eraković Mile Duka Miloš Bekić Marina Milanović Marijana Tomić Sergej Vučević Dragana Čolić Miodrag |
| author_facet | Eraković Mile Duka Miloš Bekić Marina Milanović Marijana Tomić Sergej Vučević Dragana Čolić Miodrag |
| author_sort | Eraković Mile |
| collection | DOAJ |
| description | Background/Aim. Amalgam has been used for years in dentistry, but the controversy on its adverse effects, both on local oral/dental tissues and systemic health, still exists. When used for retrograde filling in apical surgery, amalgam comes in close contact with the periapical tissue, and it is sometimes responsible for the induction of periapical lesion (PL) or its exacerbation. Therefore, the aim of the study was to examine the effect of amalgam on cytotoxicity and production of pro-inflammatory cytokine by cells isolated from PL. Methods. Conditioned medium from freshly prepared amalgam (ACM) was performed according to the ISO 10993-12 by incubating the alloy in RPMI medium (0.2 g/mL) for 3 days at 37°C. Cells were isolated from 20 human PLs after apicoectomy by collagenase/DNA-ase digestion and cultured with different dilutions of ACM. Cytotoxicity was determined by MTT assay (n = 7 cultures) and apoptosis/necrosis assays (n = 8 cultures), whereas cytokine production was measured by a Flow Cytomix Microbeads Assay (n = 8 cultures). Results. Undiluted (100%) and 75% ACM was cytotoxic due to induction of apoptosis of PL cells. Non-cytotoxic concentrations of ACM (50% and 25%) inhibited the production of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8), concentration-dependently. Conclusion. For the first time, our results showed an unexpected anti-inflammatory property of amalgam on PL cells, which could be beneficial for PL healing after apicoectomy. |
| format | Article |
| id | doaj-art-e9846ad3171440eeb1e998d8233e96a9 |
| institution | Kabale University |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-e9846ad3171440eeb1e998d8233e96a92025-08-20T03:24:37ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202021-01-0178328929510.2298/VSP190225043E0042-84501900043EAnti-inflammatory effect of amalgam on periapical lesion cells in cultureEraković Mile0Duka Miloš1Bekić Marina2https://orcid.org/0000-0002-6945-4241Milanović Marijana3Tomić Sergej4https://orcid.org/0000-0003-2570-1295Vučević Dragana5Čolić Miodrag6https://orcid.org/0000-0002-6602-9493Military Medical Academy, Clinic for Stomatology, Belgrade, SerbiaMilitary Medical Academy, Clinic for Stomatology, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Belgrade-Zemun, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Belgrade-Zemun, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Institute for the Application of Nuclear Energy, Belgrade-Zemun, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Institute for the Application of Nuclear Energy, Belgrade-Zemun, Serbia + University of East Sarajevo, Faculty of Medicine, Foča, Republic of Srpska, Bosnia and HerzegovinaBackground/Aim. Amalgam has been used for years in dentistry, but the controversy on its adverse effects, both on local oral/dental tissues and systemic health, still exists. When used for retrograde filling in apical surgery, amalgam comes in close contact with the periapical tissue, and it is sometimes responsible for the induction of periapical lesion (PL) or its exacerbation. Therefore, the aim of the study was to examine the effect of amalgam on cytotoxicity and production of pro-inflammatory cytokine by cells isolated from PL. Methods. Conditioned medium from freshly prepared amalgam (ACM) was performed according to the ISO 10993-12 by incubating the alloy in RPMI medium (0.2 g/mL) for 3 days at 37°C. Cells were isolated from 20 human PLs after apicoectomy by collagenase/DNA-ase digestion and cultured with different dilutions of ACM. Cytotoxicity was determined by MTT assay (n = 7 cultures) and apoptosis/necrosis assays (n = 8 cultures), whereas cytokine production was measured by a Flow Cytomix Microbeads Assay (n = 8 cultures). Results. Undiluted (100%) and 75% ACM was cytotoxic due to induction of apoptosis of PL cells. Non-cytotoxic concentrations of ACM (50% and 25%) inhibited the production of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8), concentration-dependently. Conclusion. For the first time, our results showed an unexpected anti-inflammatory property of amalgam on PL cells, which could be beneficial for PL healing after apicoectomy.http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900043E.pdfdental amalgamperiapical tissuecytokinescytotoxicity, immunologicinflammationapicoectomy |
| spellingShingle | Eraković Mile Duka Miloš Bekić Marina Milanović Marijana Tomić Sergej Vučević Dragana Čolić Miodrag Anti-inflammatory effect of amalgam on periapical lesion cells in culture Vojnosanitetski Pregled dental amalgam periapical tissue cytokines cytotoxicity, immunologic inflammation apicoectomy |
| title | Anti-inflammatory effect of amalgam on periapical lesion cells in culture |
| title_full | Anti-inflammatory effect of amalgam on periapical lesion cells in culture |
| title_fullStr | Anti-inflammatory effect of amalgam on periapical lesion cells in culture |
| title_full_unstemmed | Anti-inflammatory effect of amalgam on periapical lesion cells in culture |
| title_short | Anti-inflammatory effect of amalgam on periapical lesion cells in culture |
| title_sort | anti inflammatory effect of amalgam on periapical lesion cells in culture |
| topic | dental amalgam periapical tissue cytokines cytotoxicity, immunologic inflammation apicoectomy |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900043E.pdf |
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