Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study
Mona A Alrabiah,1,* Amina A Hassan,2 Murad A Mubaraki,3 Ahmed M Albarrag,4 Mohamed A Dkhil,5 Ali M Somily,4 Denis Delic,6 Taghreed A Hafiz3,* 1Microbiology and Immunology Department, King Khaled University Hospital, Riyadh, 12372, Saudi Arabia; 2National Center for Radiation Research...
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Dove Medical Press
2025-03-01
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| Series: | Infection and Drug Resistance |
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| author | Alrabiah MA Hassan AA Mubaraki MA Albarrag AM Dkhil MA Somily AM Delic D Hafiz TA |
| author_facet | Alrabiah MA Hassan AA Mubaraki MA Albarrag AM Dkhil MA Somily AM Delic D Hafiz TA |
| author_sort | Alrabiah MA |
| collection | DOAJ |
| description | Mona A Alrabiah,1,* Amina A Hassan,2 Murad A Mubaraki,3 Ahmed M Albarrag,4 Mohamed A Dkhil,5 Ali M Somily,4 Denis Delic,6 Taghreed A Hafiz3,* 1Microbiology and Immunology Department, King Khaled University Hospital, Riyadh, 12372, Saudi Arabia; 2National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt; 3Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh, 12372, Saudi Arabia; 4Pathology Department, College of Medicine, King Saud University, Riyadh, 12372, Saudi Arabia; 5Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt; 6Department of Translational Medicine & Clinical Pharmacology, Boehringer Ingelheim Pharma, Biberach, Germany*These authors contributed equally to this workCorrespondence: Taghreed A Hafiz, Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh, 12372, Saudi Arabia, Email thafiz@ksu.edu.saBackground: The rise of antibiotic resistance is a significant threat to global health, necessitating the exploration of novel antimicrobial agents. Glycerol monolaurate (GML) is known for its antimicrobial properties, primarily against Gram-positive bacteria, with limited evidence of efficacy against Gram-negative pathogens.Methods: This study evaluated the antibacterial activity of GML alone and in combination with human serum albumin (HSA) against clinical isolates of carbapenem-resistant and vancomycin-resistant bacteria using MIC and time-kill assays.Results: Contrary to previous reports, we demonstrate that GML exhibits significant antibacterial activity against Gram-negative bacteria, including strains resistant to conventional antibiotics. It inhibited carbapenem-resistant isolates with MIC values ranging from 25 to 100 μg/mL for E. coli, K. pneumoniae, and E. cloacae and showed bacteriostatic and bactericidal activity. The combination of HSA and GML enhanced this effect, showing potent bactericidal properties across all tested concentrations.Conclusion: Current findings suggest that HSA-bound GML could be developed as a novel broad-spectrum antimicrobial agent targeting multidrug-resistant pathogens. Future research should focus on formulation optimization, in vivo efficacy studies, and preclinical evaluations to determine its therapeutic potential in clinical settings.Keywords: Carbapenem-resistant, human serum albumin, MIC, time-kill assay, clinical isolates |
| format | Article |
| id | doaj-art-e9746f09fdfd4220b0c0c137f9460136 |
| institution | DOAJ |
| issn | 1178-6973 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Dove Medical Press |
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| series | Infection and Drug Resistance |
| spelling | doaj-art-e9746f09fdfd4220b0c0c137f94601362025-08-20T02:40:41ZengDove Medical PressInfection and Drug Resistance1178-69732025-03-01Volume 1815811593101427Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay StudyAlrabiah MAHassan AAMubaraki MAAlbarrag AMDkhil MASomily AMDelic DHafiz TAMona A Alrabiah,1,* Amina A Hassan,2 Murad A Mubaraki,3 Ahmed M Albarrag,4 Mohamed A Dkhil,5 Ali M Somily,4 Denis Delic,6 Taghreed A Hafiz3,* 1Microbiology and Immunology Department, King Khaled University Hospital, Riyadh, 12372, Saudi Arabia; 2National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt; 3Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh, 12372, Saudi Arabia; 4Pathology Department, College of Medicine, King Saud University, Riyadh, 12372, Saudi Arabia; 5Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt; 6Department of Translational Medicine & Clinical Pharmacology, Boehringer Ingelheim Pharma, Biberach, Germany*These authors contributed equally to this workCorrespondence: Taghreed A Hafiz, Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh, 12372, Saudi Arabia, Email thafiz@ksu.edu.saBackground: The rise of antibiotic resistance is a significant threat to global health, necessitating the exploration of novel antimicrobial agents. Glycerol monolaurate (GML) is known for its antimicrobial properties, primarily against Gram-positive bacteria, with limited evidence of efficacy against Gram-negative pathogens.Methods: This study evaluated the antibacterial activity of GML alone and in combination with human serum albumin (HSA) against clinical isolates of carbapenem-resistant and vancomycin-resistant bacteria using MIC and time-kill assays.Results: Contrary to previous reports, we demonstrate that GML exhibits significant antibacterial activity against Gram-negative bacteria, including strains resistant to conventional antibiotics. It inhibited carbapenem-resistant isolates with MIC values ranging from 25 to 100 μg/mL for E. coli, K. pneumoniae, and E. cloacae and showed bacteriostatic and bactericidal activity. The combination of HSA and GML enhanced this effect, showing potent bactericidal properties across all tested concentrations.Conclusion: Current findings suggest that HSA-bound GML could be developed as a novel broad-spectrum antimicrobial agent targeting multidrug-resistant pathogens. Future research should focus on formulation optimization, in vivo efficacy studies, and preclinical evaluations to determine its therapeutic potential in clinical settings.Keywords: Carbapenem-resistant, human serum albumin, MIC, time-kill assay, clinical isolateshttps://www.dovepress.com/unexpected-efficacy-of-albumin-bound-glycerol-monolaurate-against-mult-peer-reviewed-fulltext-article-IDRcarbapenem-resistanthuman serum albuminmictime-kill assayclinical isolates |
| spellingShingle | Alrabiah MA Hassan AA Mubaraki MA Albarrag AM Dkhil MA Somily AM Delic D Hafiz TA Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study Infection and Drug Resistance carbapenem-resistant human serum albumin mic time-kill assay clinical isolates |
| title | Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study |
| title_full | Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study |
| title_fullStr | Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study |
| title_full_unstemmed | Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study |
| title_short | Unexpected Efficacy of Albumin-Bound Glycerol Monolaurate Against Multidrug-Resistant Bacterial Isolates: A Time-Kill Assay Study |
| title_sort | unexpected efficacy of albumin bound glycerol monolaurate against multidrug resistant bacterial isolates a time kill assay study |
| topic | carbapenem-resistant human serum albumin mic time-kill assay clinical isolates |
| url | https://www.dovepress.com/unexpected-efficacy-of-albumin-bound-glycerol-monolaurate-against-mult-peer-reviewed-fulltext-article-IDR |
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