The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period
Background: The insulin-like growth factor (IGF) system plays a vital role in regulating gonadotropin-releasing hormone (GnRH), whether the IGF2 can act on the GnRH neurons during the pubertal period is unclear. Methods:...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2024-11-01
|
| Series: | Journal of Integrative Neuroscience |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/JIN/23/11/10.31083/j.jin2311208 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850218980221911040 |
|---|---|
| author | Xiaoli Dai Yuanyuan He Xinghui Han Wen Sun Jian Yu Yating Lin Yonghong Wang |
| author_facet | Xiaoli Dai Yuanyuan He Xinghui Han Wen Sun Jian Yu Yating Lin Yonghong Wang |
| author_sort | Xiaoli Dai |
| collection | DOAJ |
| description | Background: The insulin-like growth factor (IGF) system plays a vital role in regulating gonadotropin-releasing hormone (GnRH), whether the IGF2 can act on the GnRH neurons during the pubertal period is unclear. Methods: Central precocious puberty (CPP) rats were induced by danazol, and when the rats met the first diestrus, they were euthanized and tissues were collected. GT1-7 cells were cultured and treated with 0, 1, 10 ng/mL IGF2 for 4 hours and the changes in GnRH were measured. Mice were injected intracerebroventricularly with IGF2 (15 ng/g, 5 μL) or with the same dose of phosphate buffered saline (PBS), after eight hours, they were euthanized and tissues collected. Results: CPP rats had increased expression of IGF2 and GnRH mRNA and their respective proteins in the preoptic area (POA) of the hypothalamus. Treatment of GT1-7 cells with 10 ng/mL of IGF2 increased GnRH mRNA and protein expression, and GnRH concentration in the culture medium. Injection of IGF2 protein into the lateral ventricle of mice increased the expression of GnRH mRNA and protein in the POA. Conclusions: IGF2 may upregulate the synthesis of GnRH during the pubertal period, and may also take part in the pathology of CPP. |
| format | Article |
| id | doaj-art-e972935d5e434d66807e00e5d8d9fe5f |
| institution | OA Journals |
| issn | 0219-6352 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj-art-e972935d5e434d66807e00e5d8d9fe5f2025-08-20T02:07:31ZengIMR PressJournal of Integrative Neuroscience0219-63522024-11-01231120810.31083/j.jin2311208S0219-6352(24)00816-7The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal PeriodXiaoli Dai0Yuanyuan He1Xinghui Han2Wen Sun3Jian Yu4Yating Lin5Yonghong Wang6Department of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, 201102 Shanghai, ChinaBackground: The insulin-like growth factor (IGF) system plays a vital role in regulating gonadotropin-releasing hormone (GnRH), whether the IGF2 can act on the GnRH neurons during the pubertal period is unclear. Methods: Central precocious puberty (CPP) rats were induced by danazol, and when the rats met the first diestrus, they were euthanized and tissues were collected. GT1-7 cells were cultured and treated with 0, 1, 10 ng/mL IGF2 for 4 hours and the changes in GnRH were measured. Mice were injected intracerebroventricularly with IGF2 (15 ng/g, 5 μL) or with the same dose of phosphate buffered saline (PBS), after eight hours, they were euthanized and tissues collected. Results: CPP rats had increased expression of IGF2 and GnRH mRNA and their respective proteins in the preoptic area (POA) of the hypothalamus. Treatment of GT1-7 cells with 10 ng/mL of IGF2 increased GnRH mRNA and protein expression, and GnRH concentration in the culture medium. Injection of IGF2 protein into the lateral ventricle of mice increased the expression of GnRH mRNA and protein in the POA. Conclusions: IGF2 may upregulate the synthesis of GnRH during the pubertal period, and may also take part in the pathology of CPP.https://www.imrpress.com/journal/JIN/23/11/10.31083/j.jin2311208gonadotropin-releasing hormoneinsulin-like growth factor 2central precocious pubertygt1-7 |
| spellingShingle | Xiaoli Dai Yuanyuan He Xinghui Han Wen Sun Jian Yu Yating Lin Yonghong Wang The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period Journal of Integrative Neuroscience gonadotropin-releasing hormone insulin-like growth factor 2 central precocious puberty gt1-7 |
| title | The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period |
| title_full | The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period |
| title_fullStr | The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period |
| title_full_unstemmed | The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period |
| title_short | The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period |
| title_sort | regulatory effect of insulin like growth factor 2 on hypothalamic gonadotropin releasing hormone neurons during the pubertal period |
| topic | gonadotropin-releasing hormone insulin-like growth factor 2 central precocious puberty gt1-7 |
| url | https://www.imrpress.com/journal/JIN/23/11/10.31083/j.jin2311208 |
| work_keys_str_mv | AT xiaolidai theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT yuanyuanhe theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT xinghuihan theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT wensun theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT jianyu theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT yatinglin theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT yonghongwang theregulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT xiaolidai regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT yuanyuanhe regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT xinghuihan regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT wensun regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT jianyu regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT yatinglin regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod AT yonghongwang regulatoryeffectofinsulinlikegrowthfactor2onhypothalamicgonadotropinreleasinghormoneneuronsduringthepubertalperiod |