Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon
The egg-laying interval reflects the laying performance of pigeons and is affected by the mechanism of ovulation. The Forkhead box O3 (FOXO3) gene is closely associated with follicular development, playing roles in the regulation of follicular granulocyte proliferation, differentiation and periodic...
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| Language: | English |
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Elsevier
2025-08-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125005772 |
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| author | Qiaoqiao Peng Junyuan Lin Yuling Wu Zengguang Liao Lihong Gu Lizhi Lu Li Zhang |
| author_facet | Qiaoqiao Peng Junyuan Lin Yuling Wu Zengguang Liao Lihong Gu Lizhi Lu Li Zhang |
| author_sort | Qiaoqiao Peng |
| collection | DOAJ |
| description | The egg-laying interval reflects the laying performance of pigeons and is affected by the mechanism of ovulation. The Forkhead box O3 (FOXO3) gene is closely associated with follicular development, playing roles in the regulation of follicular granulocyte proliferation, differentiation and periodic ovulation. The objective of this research was to analyze associations of polymorphisms of FOXO3 gene with egg-laying intervals in pigeon. A total of 250 female pigeons of Tianxiang 1 strain were used as experimental materials. Primers were designed according to the sequence of FOXO3 gene in pigeons, and single nucleotide polymorphisms (SNP) of FOXO3 gene were detected by sequencing of PCR products. Twenty SNP sites were found, and 7 of them were located in exons, 1 of them was located in intron, and 12 of them were in the 3′ untranslated region (3′UTR) of the gene. Linkage disequilibrium (LD) analysis showed that four blocks were constructed from 20 SNPs. Association analysis showed that 6 SNPs (g.3149417 G > A, g.3149444 C > T, g.3149486 A > G, g.3150038 T > C, g.3151252 G > T and g.3152087 A > G) were significantly associated with egg-laying intervals. Furthermore, we found that the haplotypes of block1 and block4 were significantly correlated with egg-laying intervals. These results suggested that the FOXO3 gene may serve as a candidate gene for laying performance of pigeon. |
| format | Article |
| id | doaj-art-e96f55be03aa462ca5d5eb3dbca41176 |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e96f55be03aa462ca5d5eb3dbca411762025-08-20T03:08:22ZengElsevierPoultry Science0032-57912025-08-01104810533410.1016/j.psj.2025.105334Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeonQiaoqiao Peng0Junyuan Lin1Yuling Wu2Zengguang Liao3Lihong Gu4Lizhi Lu5Li Zhang6College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, ChinaInstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; Corresponding author.The egg-laying interval reflects the laying performance of pigeons and is affected by the mechanism of ovulation. The Forkhead box O3 (FOXO3) gene is closely associated with follicular development, playing roles in the regulation of follicular granulocyte proliferation, differentiation and periodic ovulation. The objective of this research was to analyze associations of polymorphisms of FOXO3 gene with egg-laying intervals in pigeon. A total of 250 female pigeons of Tianxiang 1 strain were used as experimental materials. Primers were designed according to the sequence of FOXO3 gene in pigeons, and single nucleotide polymorphisms (SNP) of FOXO3 gene were detected by sequencing of PCR products. Twenty SNP sites were found, and 7 of them were located in exons, 1 of them was located in intron, and 12 of them were in the 3′ untranslated region (3′UTR) of the gene. Linkage disequilibrium (LD) analysis showed that four blocks were constructed from 20 SNPs. Association analysis showed that 6 SNPs (g.3149417 G > A, g.3149444 C > T, g.3149486 A > G, g.3150038 T > C, g.3151252 G > T and g.3152087 A > G) were significantly associated with egg-laying intervals. Furthermore, we found that the haplotypes of block1 and block4 were significantly correlated with egg-laying intervals. These results suggested that the FOXO3 gene may serve as a candidate gene for laying performance of pigeon.http://www.sciencedirect.com/science/article/pii/S0032579125005772FOXO3 geneSingle nucleotide polymorphismsPigeonEgg-laying intervalCorrelation analysis |
| spellingShingle | Qiaoqiao Peng Junyuan Lin Yuling Wu Zengguang Liao Lihong Gu Lizhi Lu Li Zhang Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon Poultry Science FOXO3 gene Single nucleotide polymorphisms Pigeon Egg-laying interval Correlation analysis |
| title | Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon |
| title_full | Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon |
| title_fullStr | Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon |
| title_full_unstemmed | Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon |
| title_short | Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon |
| title_sort | association of foxo3 gene polymorphisms with egg laying intervals in pigeon |
| topic | FOXO3 gene Single nucleotide polymorphisms Pigeon Egg-laying interval Correlation analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125005772 |
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