Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh
Background: Klebsiella pneumoniae (Kpn), a WHO priority pathogen with high rates of antimicrobial resistance (AMR), has emerged as a leading cause of hospital acquired pneumonia and neonatal sepsis. Objective: We aimed to define the clinical characteristics of a cohort of patients with Kpn infection...
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Elsevier
2025-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004831 |
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| author | Zannat Kawser Sushmita Sridhar Sanchita Kar Tanbir Habib Sharmin Akter Mukta Kasrina Azad Neyamul Hasan Umme Kulsum Abu Bakar Siddik Saikt Rahman Nusrat Noor Tanni Maherun Nesa Ashlee M. Earl Colin J. Worby Sarah E. Turbett SM Shamsuzzaman Jason B Harris Firdausi Qadri Regina C LaRocque |
| author_facet | Zannat Kawser Sushmita Sridhar Sanchita Kar Tanbir Habib Sharmin Akter Mukta Kasrina Azad Neyamul Hasan Umme Kulsum Abu Bakar Siddik Saikt Rahman Nusrat Noor Tanni Maherun Nesa Ashlee M. Earl Colin J. Worby Sarah E. Turbett SM Shamsuzzaman Jason B Harris Firdausi Qadri Regina C LaRocque |
| author_sort | Zannat Kawser |
| collection | DOAJ |
| description | Background: Klebsiella pneumoniae (Kpn), a WHO priority pathogen with high rates of antimicrobial resistance (AMR), has emerged as a leading cause of hospital acquired pneumonia and neonatal sepsis. Objective: We aimed to define the clinical characteristics of a cohort of patients with Kpn infection in Dhaka, Bangladesh and to perform phenotypic and genetic characterization of the associated isolates. Methods: We retrospectively extracted clinical data about patients at Dhaka Medical College Hospital from whom Klebsiella spp was isolated from a clinical specimen collected between February and September 2022. We used standard microbiologic techniques to evaluate AMR and whole-genome sequencing (WGS) to assess dominant lineages, common capsular (K) and O-polysaccharide (O) antigen types, and AMR and virulence genes. Results: Ninety-eight patients were included, with diagnoses of pneumonia (38/98, 39 %), wound infection (29/98, 31 %), urinary tract infection (29/98, 31 %) and bacteremia (2/98, 2 %). We tested isolates for susceptibility to eight classes of antibiotics. Of the 98 isolates, 41 % were multidrug resistant (MDR), 15 % were extensively drug resistant (XDR), and 16 % were pan-drug resistant (PDR). Three isolates (3 %) were resistant to polymyxin B. Outcome data were available for 46 patients; 4 patients (8 %) died from infections caused by PDR (n = 2), XDR (n = 1), and MDR isolates (n = 1). WGS revealed a high degree of genomic diversity, with multiple sequence types (STs), O-types and K-types represented; ST16:K81:OL101 and ST43:K30:O1 were the most prevalent. Conclusion: Our findings suggest alarming levels of AMR among Kpn isolates in Bangladesh and a critical need for improved treatment modalities and vaccine development. |
| format | Article |
| id | doaj-art-e96702cd19c041a5826333fafba0b7d3 |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-e96702cd19c041a5826333fafba0b7d32025-08-20T01:57:36ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-03-0141525810.1016/j.jgar.2024.12.016Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, BangladeshZannat Kawser0Sushmita Sridhar1Sanchita Kar2Tanbir Habib3Sharmin Akter Mukta4Kasrina Azad5Neyamul Hasan6Umme Kulsum7Abu Bakar Siddik8Saikt Rahman9Nusrat Noor Tanni10Maherun Nesa11Ashlee M. Earl12Colin J. Worby13Sarah E. Turbett14SM Shamsuzzaman15Jason B Harris16Firdausi Qadri17Regina C LaRocque18institute for developing Science and Health initiatives, Dhaka, Bangladesh; Zannat Kawser, Sushmita Sridhar and Sanchita Kar contributed equally to this work.Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA USA; Zannat Kawser, Sushmita Sridhar and Sanchita Kar contributed equally to this work.institute for developing Science and Health initiatives, Dhaka, Bangladesh; Zannat Kawser, Sushmita Sridhar and Sanchita Kar contributed equally to this work.institute for developing Science and Health initiatives, Dhaka, Bangladeshinstitute for developing Science and Health initiatives, Dhaka, Bangladeshinstitute for developing Science and Health initiatives, Dhaka, Bangladeshinstitute for developing Science and Health initiatives, Dhaka, Bangladeshinstitute for developing Science and Health initiatives, Dhaka, Bangladeshinstitute for developing Science and Health initiatives, Dhaka, Bangladeshinstitute for developing Science and Health initiatives, Dhaka, BangladeshDhaka Medical College and Hospital, Dhaka, BangladeshDhaka Medical College and Hospital, Dhaka, BangladeshInfectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA USAInfectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA USADivision of Infectious Diseases, Massachusetts General Hospital, Boston, MA USA; Harvard Medical School, Boston, MA USA; Department of Pathology, Massachusetts General Hospital, Boston, MA USADhaka Medical College and Hospital, Dhaka, BangladeshHarvard Medical School, Boston, MA USA; Massachusetts General Hospital for Children, Boston, MA USAinstitute for developing Science and Health initiatives, Dhaka, Bangladesh; International Centre for Diarrhoeal Disease Research, Dhaka, BangladeshDivision of Infectious Diseases, Massachusetts General Hospital, Boston, MA USA; Harvard Medical School, Boston, MA USA; Corresponding author. Mailing address: Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA.Background: Klebsiella pneumoniae (Kpn), a WHO priority pathogen with high rates of antimicrobial resistance (AMR), has emerged as a leading cause of hospital acquired pneumonia and neonatal sepsis. Objective: We aimed to define the clinical characteristics of a cohort of patients with Kpn infection in Dhaka, Bangladesh and to perform phenotypic and genetic characterization of the associated isolates. Methods: We retrospectively extracted clinical data about patients at Dhaka Medical College Hospital from whom Klebsiella spp was isolated from a clinical specimen collected between February and September 2022. We used standard microbiologic techniques to evaluate AMR and whole-genome sequencing (WGS) to assess dominant lineages, common capsular (K) and O-polysaccharide (O) antigen types, and AMR and virulence genes. Results: Ninety-eight patients were included, with diagnoses of pneumonia (38/98, 39 %), wound infection (29/98, 31 %), urinary tract infection (29/98, 31 %) and bacteremia (2/98, 2 %). We tested isolates for susceptibility to eight classes of antibiotics. Of the 98 isolates, 41 % were multidrug resistant (MDR), 15 % were extensively drug resistant (XDR), and 16 % were pan-drug resistant (PDR). Three isolates (3 %) were resistant to polymyxin B. Outcome data were available for 46 patients; 4 patients (8 %) died from infections caused by PDR (n = 2), XDR (n = 1), and MDR isolates (n = 1). WGS revealed a high degree of genomic diversity, with multiple sequence types (STs), O-types and K-types represented; ST16:K81:OL101 and ST43:K30:O1 were the most prevalent. Conclusion: Our findings suggest alarming levels of AMR among Kpn isolates in Bangladesh and a critical need for improved treatment modalities and vaccine development.http://www.sciencedirect.com/science/article/pii/S2213716524004831Klebsiella pneumoniaeAntimicrobial resistanceBacterial virulenceSequence types |
| spellingShingle | Zannat Kawser Sushmita Sridhar Sanchita Kar Tanbir Habib Sharmin Akter Mukta Kasrina Azad Neyamul Hasan Umme Kulsum Abu Bakar Siddik Saikt Rahman Nusrat Noor Tanni Maherun Nesa Ashlee M. Earl Colin J. Worby Sarah E. Turbett SM Shamsuzzaman Jason B Harris Firdausi Qadri Regina C LaRocque Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh Journal of Global Antimicrobial Resistance Klebsiella pneumoniae Antimicrobial resistance Bacterial virulence Sequence types |
| title | Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh |
| title_full | Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh |
| title_fullStr | Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh |
| title_full_unstemmed | Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh |
| title_short | Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh |
| title_sort | clinical and genomic characterization of klebsiella pneumoniae infections in dhaka bangladesh |
| topic | Klebsiella pneumoniae Antimicrobial resistance Bacterial virulence Sequence types |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004831 |
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