Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China
The aim of this study was to better understand the sequence characteristics and immune responses in avian leukosis virus subgroup J (ALV-J) infected yellow chicken flocks in South China. We isolated four strains of ALV-J virus from these flocks, which were then identified by several methods, includi...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/6665871 |
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| author | Qihong Zhang Guodong Mo Tingting Xie Zihao Zhang Huali Fu Ping Wei Xiquan Zhang |
| author_facet | Qihong Zhang Guodong Mo Tingting Xie Zihao Zhang Huali Fu Ping Wei Xiquan Zhang |
| author_sort | Qihong Zhang |
| collection | DOAJ |
| description | The aim of this study was to better understand the sequence characteristics and immune responses in avian leukosis virus subgroup J (ALV-J) infected yellow chicken flocks in South China. We isolated four strains of ALV-J virus from these flocks, which were then identified by several methods, including subtype-specific polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assay (IFA). All four viruses were sequenced for their complete genomes and named GD19GZ01, GD19GZ02, GD19GZ03, and GD19GZ04. In comparison with the reference sequence, the homology analysis showed that the gag and pol genes were relatively conserved, whereas env contained much variation. Both GD19GZ01 and GD19GZ02 almost entirely lacked the rTM region and E element, while the latter was retained in GD19GZ03 and GD19GZ04. Moreover, the virus replication levels in GD19GZ03 and GD19GZ04were much higher than those in GD19GZ01 and GD19GZ02. And three virus recombination events in GD19GZ01 and GD19GZ02 were revealed by the results of PDR5 and SimPlot software analysis. Additionally, we found that some interferon-stimulating genes (CH25H, MX, PKR, OAS, and ZAP) and inflammatory mediators (IL-4, IL-6, IL-10, IL-12, 1L-18, and TNF-α) were significantly upregulated in the immune system organs of clinical chickens. Taken together, these findings clarify and reveal the sequence characteristics and trends in the variation of ALV-J infection in yellow chicken flocks of South China. |
| format | Article |
| id | doaj-art-e959ed98e870492497bacc6688d96014 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e959ed98e870492497bacc6688d960142025-08-20T02:22:15ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/66658716665871Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South ChinaQihong Zhang0Guodong Mo1Tingting Xie2Zihao Zhang3Huali Fu4Ping Wei5Xiquan Zhang6Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaThe aim of this study was to better understand the sequence characteristics and immune responses in avian leukosis virus subgroup J (ALV-J) infected yellow chicken flocks in South China. We isolated four strains of ALV-J virus from these flocks, which were then identified by several methods, including subtype-specific polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assay (IFA). All four viruses were sequenced for their complete genomes and named GD19GZ01, GD19GZ02, GD19GZ03, and GD19GZ04. In comparison with the reference sequence, the homology analysis showed that the gag and pol genes were relatively conserved, whereas env contained much variation. Both GD19GZ01 and GD19GZ02 almost entirely lacked the rTM region and E element, while the latter was retained in GD19GZ03 and GD19GZ04. Moreover, the virus replication levels in GD19GZ03 and GD19GZ04were much higher than those in GD19GZ01 and GD19GZ02. And three virus recombination events in GD19GZ01 and GD19GZ02 were revealed by the results of PDR5 and SimPlot software analysis. Additionally, we found that some interferon-stimulating genes (CH25H, MX, PKR, OAS, and ZAP) and inflammatory mediators (IL-4, IL-6, IL-10, IL-12, 1L-18, and TNF-α) were significantly upregulated in the immune system organs of clinical chickens. Taken together, these findings clarify and reveal the sequence characteristics and trends in the variation of ALV-J infection in yellow chicken flocks of South China.http://dx.doi.org/10.1155/2021/6665871 |
| spellingShingle | Qihong Zhang Guodong Mo Tingting Xie Zihao Zhang Huali Fu Ping Wei Xiquan Zhang Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China Mediators of Inflammation |
| title | Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China |
| title_full | Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China |
| title_fullStr | Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China |
| title_full_unstemmed | Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China |
| title_short | Phylogenetic Analysis of ALV-J Associated with Immune Responses in Yellow Chicken Flocks in South China |
| title_sort | phylogenetic analysis of alv j associated with immune responses in yellow chicken flocks in south china |
| url | http://dx.doi.org/10.1155/2021/6665871 |
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