Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB
Polyvinyl chloride (PVC) stands as the third most widely produced synthetic polymer plastic. However, investigations into its microbial degradation significantly trail those of polyethylene (PE) and polystyrene (PS). Here, this study enriched a PVC-degrading consortium DC from the soil microbes of T...
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Elsevier
2025-08-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412025004593 |
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| author | Yuchao Jiang Baotong Fu Wei-Min Wu Chunxiu Zhou Aman Khan Guorui Zhang El-Sayed Salama Byong-Hun Jeon Maha Awjan Alreshidi Chunjie Li Huawen Han Xiangkai Li |
| author_facet | Yuchao Jiang Baotong Fu Wei-Min Wu Chunxiu Zhou Aman Khan Guorui Zhang El-Sayed Salama Byong-Hun Jeon Maha Awjan Alreshidi Chunjie Li Huawen Han Xiangkai Li |
| author_sort | Yuchao Jiang |
| collection | DOAJ |
| description | Polyvinyl chloride (PVC) stands as the third most widely produced synthetic polymer plastic. However, investigations into its microbial degradation significantly trail those of polyethylene (PE) and polystyrene (PS). Here, this study enriched a PVC-degrading consortium DC from the soil microbes of Tibet Plateau grasslands, confirming the formation of long-chain alkanes and the release of chloride ions via GC–MS and ion chromatography analysis. The response of DC to synthetic polymer PVC and natural polymer lignin revealed resistant polymer-response Burkholderia, opportunistic polymer-response Rhodococcus, and sensitive polymer-response Dyella. These PVC-degrading bacteria exist in 432 datasets of alpine meadows. Metaproteomic analysis highlighted distinct enzymatic profiles between PVC-DC and lignin-DC, with 298 proteins associated with PVC degradation compared to 123 proteins linked to lignin. Six key protein categories, including dehalogenase, peroxidase, monooxygenase, dioxygenase, esterase, and dehydrogenase, were involved in PVC degradation. An upregulated dehalogenase SerB from Rhodococcus, belonging to the clade of phosphoserine phosphatase, displayed proficiency in degrading chloroacetic acid and PVC films via hydrolytic dehalogenation. Furthermore, global metagenomic analysis from 38 datasets underscored the prevalence of dehalogenases and related enzymes in grasslands. This study elucidates the microbial responses and enzymatic pathways in PVC degradation, emphasizing the abundance of PVC-degrading enzymes in alpine ecosystems. |
| format | Article |
| id | doaj-art-e952b1656c854765a99bb49952302732 |
| institution | Kabale University |
| issn | 0160-4120 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj-art-e952b1656c854765a99bb499523027322025-08-20T03:33:04ZengElsevierEnvironment International0160-41202025-08-0120210970810.1016/j.envint.2025.109708Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerBYuchao Jiang0Baotong Fu1Wei-Min Wu2Chunxiu Zhou3Aman Khan4Guorui Zhang5El-Sayed Salama6Byong-Hun Jeon7Maha Awjan Alreshidi8Chunjie Li9Huawen Han10Xiangkai Li11Centre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, China; Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, ChinaCentre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, ChinaDepartment of Civil and Environmental Engineering, William & Cloy Codiga Resource Recovery Center, Stanford University, Stanford, CA 94305, United StatesCentre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCentre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, ChinaDepartment of Occupational and Environmental Health, School of Public Health, Lanzhou University, Lanzhou 730000 Gansu, ChinaDepartment of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, South KoreaDepartment of Chemistry, College of Science, University of Ha’il, Ha’il 81451, Saudi ArabiaCentre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, China; Corresponding authors.Centre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, China; Corresponding authors.Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, ChinaPolyvinyl chloride (PVC) stands as the third most widely produced synthetic polymer plastic. However, investigations into its microbial degradation significantly trail those of polyethylene (PE) and polystyrene (PS). Here, this study enriched a PVC-degrading consortium DC from the soil microbes of Tibet Plateau grasslands, confirming the formation of long-chain alkanes and the release of chloride ions via GC–MS and ion chromatography analysis. The response of DC to synthetic polymer PVC and natural polymer lignin revealed resistant polymer-response Burkholderia, opportunistic polymer-response Rhodococcus, and sensitive polymer-response Dyella. These PVC-degrading bacteria exist in 432 datasets of alpine meadows. Metaproteomic analysis highlighted distinct enzymatic profiles between PVC-DC and lignin-DC, with 298 proteins associated with PVC degradation compared to 123 proteins linked to lignin. Six key protein categories, including dehalogenase, peroxidase, monooxygenase, dioxygenase, esterase, and dehydrogenase, were involved in PVC degradation. An upregulated dehalogenase SerB from Rhodococcus, belonging to the clade of phosphoserine phosphatase, displayed proficiency in degrading chloroacetic acid and PVC films via hydrolytic dehalogenation. Furthermore, global metagenomic analysis from 38 datasets underscored the prevalence of dehalogenases and related enzymes in grasslands. This study elucidates the microbial responses and enzymatic pathways in PVC degradation, emphasizing the abundance of PVC-degrading enzymes in alpine ecosystems.http://www.sciencedirect.com/science/article/pii/S0160412025004593PVCBiodegradationQinghai-Tibet PlateauDehalogenaseMetaproteomics |
| spellingShingle | Yuchao Jiang Baotong Fu Wei-Min Wu Chunxiu Zhou Aman Khan Guorui Zhang El-Sayed Salama Byong-Hun Jeon Maha Awjan Alreshidi Chunjie Li Huawen Han Xiangkai Li Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB Environment International PVC Biodegradation Qinghai-Tibet Plateau Dehalogenase Metaproteomics |
| title | Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB |
| title_full | Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB |
| title_fullStr | Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB |
| title_full_unstemmed | Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB |
| title_short | Soil microbes in the Tibetan Plateau degrade polyvinyl chloride and harbor novel dehalogenase SerB |
| title_sort | soil microbes in the tibetan plateau degrade polyvinyl chloride and harbor novel dehalogenase serb |
| topic | PVC Biodegradation Qinghai-Tibet Plateau Dehalogenase Metaproteomics |
| url | http://www.sciencedirect.com/science/article/pii/S0160412025004593 |
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