Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected
To investigate effect of MMLA, an inhibitor of nitric oxide (NO) production, on regulation of inflammatory responses to Bordetella pertussis infection, mice were infected intranasally, and treated with various concentrations of MMLA. Ten days after infection, mice treated with MMLA at dosage of 100...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1999-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359990685 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850164719371943936 |
|---|---|
| author | D. Torre F. Speranza A. Pugliese G. Fassina A. Osculati L. Perversi M. G. Banfi M. Airoldi |
| author_facet | D. Torre F. Speranza A. Pugliese G. Fassina A. Osculati L. Perversi M. G. Banfi M. Airoldi |
| author_sort | D. Torre |
| collection | DOAJ |
| description | To investigate effect of MMLA, an inhibitor of nitric oxide (NO) production, on regulation of inflammatory responses to Bordetella pertussis infection, mice were infected intranasally, and treated with various concentrations of MMLA. Ten days after infection, mice treated with MMLA at dosage of 100 mg/kg, given intraperitoneally in a single dose or for 5 consecutive days, showed at histopathologic examination, a significant decrease of intensity of inflammation (scores, 0.6±0.2 and 0.9±0.5 respectively). A decrease of cellular accumulation of neutrophils and lymphocytes in the bronchoalveolar lavage (BAL) fluid was observed in infected mice treated with MMLA, especially at dosage of 10 mg/kg, given in a single dose intraperitoneally. In addition, BP-infected mice treated with MMLA (100 mg/kg, intraperitoneally) for 5 consecutive days showed higher mortality rate than untreated mice infected with B. pertussis, and the number of B. pertussis in lungs of mice treated with MMLA was significantly increased. However, MMLA treatment of infected mice had some effect on levels of IFN-γ and nitrite/nitrate (end-stable products of NO) in the BAL fluid. This study indicates that NO may play a role either as microbiocidal agent or as a modulator of immune regulation, inasmuch as it may upregulate tissue inflammatory response to B.pertussis. |
| format | Article |
| id | doaj-art-e94e358c39fb442cad776284da90ee75 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e94e358c39fb442cad776284da90ee752025-08-20T02:21:53ZengWileyMediators of Inflammation0962-93511466-18611999-01-0181252910.1080/09629359990685Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally InfectedD. Torre0F. Speranza1A. Pugliese2G. Fassina3A. Osculati4L. Perversi5M. G. Banfi6M. Airoldi7Division of Infectious Diseases, Regional Hospital, Viale Borri 57, Varese 21100, ItalyDivision of Infectious Diseases, Regional Hospital, Viale Borri 57, Varese 21100, ItalyInstitute of Infectious Diseases, University of Turin, Turin 10100, ItalyInstitute of Forensic Medicine, University of Pavia, ItalyInstitute of Forensic Medicine, University of Pavia, ItalyInstitute of Infectious Diseases, University of Pavia, Pavia 27100, ItalyDivision of Infectious Diseases, Regional Hospital, Viale Borri 57, Varese 21100, ItalyDivision of Infectious Diseases, Regional Hospital, Viale Borri 57, Varese 21100, ItalyTo investigate effect of MMLA, an inhibitor of nitric oxide (NO) production, on regulation of inflammatory responses to Bordetella pertussis infection, mice were infected intranasally, and treated with various concentrations of MMLA. Ten days after infection, mice treated with MMLA at dosage of 100 mg/kg, given intraperitoneally in a single dose or for 5 consecutive days, showed at histopathologic examination, a significant decrease of intensity of inflammation (scores, 0.6±0.2 and 0.9±0.5 respectively). A decrease of cellular accumulation of neutrophils and lymphocytes in the bronchoalveolar lavage (BAL) fluid was observed in infected mice treated with MMLA, especially at dosage of 10 mg/kg, given in a single dose intraperitoneally. In addition, BP-infected mice treated with MMLA (100 mg/kg, intraperitoneally) for 5 consecutive days showed higher mortality rate than untreated mice infected with B. pertussis, and the number of B. pertussis in lungs of mice treated with MMLA was significantly increased. However, MMLA treatment of infected mice had some effect on levels of IFN-γ and nitrite/nitrate (end-stable products of NO) in the BAL fluid. This study indicates that NO may play a role either as microbiocidal agent or as a modulator of immune regulation, inasmuch as it may upregulate tissue inflammatory response to B.pertussis.http://dx.doi.org/10.1080/09629359990685 |
| spellingShingle | D. Torre F. Speranza A. Pugliese G. Fassina A. Osculati L. Perversi M. G. Banfi M. Airoldi Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected Mediators of Inflammation |
| title | Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected |
| title_full | Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected |
| title_fullStr | Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected |
| title_full_unstemmed | Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected |
| title_short | Regulation of Inflammatory Responses to Bordetella Pertussis by NG-Monomethyl-L-Arginine in Mice Intranasally Infected |
| title_sort | regulation of inflammatory responses to bordetella pertussis by ng monomethyl l arginine in mice intranasally infected |
| url | http://dx.doi.org/10.1080/09629359990685 |
| work_keys_str_mv | AT dtorre regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT fsperanza regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT apugliese regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT gfassina regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT aosculati regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT lperversi regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT mgbanfi regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected AT mairoldi regulationofinflammatoryresponsestobordetellapertussisbyngmonomethyllarginineinmiceintranasallyinfected |