Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway
Hepatic ischemia-reperfusion (IR) injury is a clinically significant process that frequently occurs in liver transplantation, partial hepatectomy, and hemorrhagic shock. The aim of this study was to explore the effectiveness of luteolin in hepatic IR injury and the underlying mechanism. BALB/c mice...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2022/8161946 |
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| _version_ | 1832565685684797440 |
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| author | Yuhui Jiang Wenjuan Yang Jiameng Ding Jie Ji Liwei Wu Yuanyuan Zheng Yan Li Ziqi Cheng Jie Zhang Qiang Yu Jiao Feng Jingjing Li Jianye Wu Yingqun Zhou Chuanyong Guo |
| author_facet | Yuhui Jiang Wenjuan Yang Jiameng Ding Jie Ji Liwei Wu Yuanyuan Zheng Yan Li Ziqi Cheng Jie Zhang Qiang Yu Jiao Feng Jingjing Li Jianye Wu Yingqun Zhou Chuanyong Guo |
| author_sort | Yuhui Jiang |
| collection | DOAJ |
| description | Hepatic ischemia-reperfusion (IR) injury is a clinically significant process that frequently occurs in liver transplantation, partial hepatectomy, and hemorrhagic shock. The aim of this study was to explore the effectiveness of luteolin in hepatic IR injury and the underlying mechanism. BALB/c mice were randomly divided into six groups, including normal controls (NC), luteolin (50 mg/kg), sham procedure, IR+25 mg/kg luteolin, and IR+50 mg/kg luteolin group. Serum and tissue samples were collected at 6 and 24 h after reperfusion to assay liver enzymes, inflammatory factors, expression of proteins associated with apoptosis and autophagy, and factors associated with the extracellular signal-regulated kinase/peroxisome proliferator-activated receptor alpha (ERK/PPARα) pathway. Luteolin preconditioning decreased hepatocyte injury caused by ischemia-reperfusion, downregulated inflammatory factors, and inhibited apoptosis and autophagy. Luteolin also inhibited ERK phosphorylation and activated PPARα. |
| format | Article |
| id | doaj-art-e94db8358e7e468bbc538c04f8931728 |
| institution | Kabale University |
| issn | 1687-4765 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-e94db8358e7e468bbc538c04f89317282025-02-03T01:06:58ZengWileyPPAR Research1687-47652022-01-01202210.1155/2022/8161946Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα PathwayYuhui Jiang0Wenjuan Yang1Jiameng Ding2Jie Ji3Liwei Wu4Yuanyuan Zheng5Yan Li6Ziqi Cheng7Jie Zhang8Qiang Yu9Jiao Feng10Jingjing Li11Jianye Wu12Yingqun Zhou13Chuanyong Guo14Department of GastroenterologyDepartment of EmergencyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyHepatic ischemia-reperfusion (IR) injury is a clinically significant process that frequently occurs in liver transplantation, partial hepatectomy, and hemorrhagic shock. The aim of this study was to explore the effectiveness of luteolin in hepatic IR injury and the underlying mechanism. BALB/c mice were randomly divided into six groups, including normal controls (NC), luteolin (50 mg/kg), sham procedure, IR+25 mg/kg luteolin, and IR+50 mg/kg luteolin group. Serum and tissue samples were collected at 6 and 24 h after reperfusion to assay liver enzymes, inflammatory factors, expression of proteins associated with apoptosis and autophagy, and factors associated with the extracellular signal-regulated kinase/peroxisome proliferator-activated receptor alpha (ERK/PPARα) pathway. Luteolin preconditioning decreased hepatocyte injury caused by ischemia-reperfusion, downregulated inflammatory factors, and inhibited apoptosis and autophagy. Luteolin also inhibited ERK phosphorylation and activated PPARα.http://dx.doi.org/10.1155/2022/8161946 |
| spellingShingle | Yuhui Jiang Wenjuan Yang Jiameng Ding Jie Ji Liwei Wu Yuanyuan Zheng Yan Li Ziqi Cheng Jie Zhang Qiang Yu Jiao Feng Jingjing Li Jianye Wu Yingqun Zhou Chuanyong Guo Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway PPAR Research |
| title | Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway |
| title_full | Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway |
| title_fullStr | Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway |
| title_full_unstemmed | Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway |
| title_short | Luteolin Pretreatment Attenuates Hepatic Ischemia-Reperfusion Injury in Mice by Inhibiting Inflammation, Autophagy, and Apoptosis via the ERK/PPARα Pathway |
| title_sort | luteolin pretreatment attenuates hepatic ischemia reperfusion injury in mice by inhibiting inflammation autophagy and apoptosis via the erk pparα pathway |
| url | http://dx.doi.org/10.1155/2022/8161946 |
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