3D tumor cultures for drug resistance and screening development in clinical applications

Abstract Tumor drug resistance presents a growing challenge in medical practice, particularly during anti-cancer therapies, where the emergence of drug-resistant cancer cells significantly complicates clinical treatment. In recent years, three-dimensional (3D) tumor culture technology, which more ef...

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Main Authors: Zheng Peng, Xiaolan Lv, Hao Sun, Lina Zhao, Shigao Huang
Format: Article
Language:English
Published: BMC 2025-03-01
Series:Molecular Cancer
Subjects:
Online Access:https://doi.org/10.1186/s12943-025-02281-2
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author Zheng Peng
Xiaolan Lv
Hao Sun
Lina Zhao
Shigao Huang
author_facet Zheng Peng
Xiaolan Lv
Hao Sun
Lina Zhao
Shigao Huang
author_sort Zheng Peng
collection DOAJ
description Abstract Tumor drug resistance presents a growing challenge in medical practice, particularly during anti-cancer therapies, where the emergence of drug-resistant cancer cells significantly complicates clinical treatment. In recent years, three-dimensional (3D) tumor culture technology, which more effectively simulates the in vivo physiological environment, has gained increasing attention in tumor drug resistance research and clinical applications. By mimicking the in vivo cellular microenvironment, 3D tumor culture technology not only recapitulates cell-cell interactions but also more faithfully reproduces the biological effects of therapeutic agents. Consequently, 3D tumor culture technology is emerging as a crucial tool in biomedical and clinical research. We summarize the benefits of 3D culture models and organoid technology, explore their application in the realm of drug resistance, drug screening, and personalized therapy, and discuss their potential application prospects and challenges in clinical transformation, with the aim of providing insights for optimizing cancer treatment strategies and advancing precision therapy.
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institution Kabale University
issn 1476-4598
language English
publishDate 2025-03-01
publisher BMC
record_format Article
series Molecular Cancer
spelling doaj-art-e94c725bd5d442f6bb9b780b02b31efd2025-08-20T03:41:49ZengBMCMolecular Cancer1476-45982025-03-0124111610.1186/s12943-025-02281-23D tumor cultures for drug resistance and screening development in clinical applicationsZheng Peng0Xiaolan Lv1Hao Sun2Lina Zhao3Shigao Huang4Department of Clinical Laboratory, Liuzhou Traditional Chinese Medical HospitalDepartment of Clinical Laboratory, Liuzhou Maternity and Child Healthcare HospitalFaculty of Science, Autonomous University of Madrid, Spainish National Research Council -Consejo Superior de Investigaciones Científicas,(UAM-CSIC)State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers, Department of Radiation Oncology, Xijing Hospital, Fourth Military Medical UniversityDepartment of Radiation Oncology, Xijing Hospital, Fourth Military Medical UniversityAbstract Tumor drug resistance presents a growing challenge in medical practice, particularly during anti-cancer therapies, where the emergence of drug-resistant cancer cells significantly complicates clinical treatment. In recent years, three-dimensional (3D) tumor culture technology, which more effectively simulates the in vivo physiological environment, has gained increasing attention in tumor drug resistance research and clinical applications. By mimicking the in vivo cellular microenvironment, 3D tumor culture technology not only recapitulates cell-cell interactions but also more faithfully reproduces the biological effects of therapeutic agents. Consequently, 3D tumor culture technology is emerging as a crucial tool in biomedical and clinical research. We summarize the benefits of 3D culture models and organoid technology, explore their application in the realm of drug resistance, drug screening, and personalized therapy, and discuss their potential application prospects and challenges in clinical transformation, with the aim of providing insights for optimizing cancer treatment strategies and advancing precision therapy.https://doi.org/10.1186/s12943-025-02281-2Cancer resistance3D tumor culture modelOrganoidsTumor microenvironmentDrug screening
spellingShingle Zheng Peng
Xiaolan Lv
Hao Sun
Lina Zhao
Shigao Huang
3D tumor cultures for drug resistance and screening development in clinical applications
Molecular Cancer
Cancer resistance
3D tumor culture model
Organoids
Tumor microenvironment
Drug screening
title 3D tumor cultures for drug resistance and screening development in clinical applications
title_full 3D tumor cultures for drug resistance and screening development in clinical applications
title_fullStr 3D tumor cultures for drug resistance and screening development in clinical applications
title_full_unstemmed 3D tumor cultures for drug resistance and screening development in clinical applications
title_short 3D tumor cultures for drug resistance and screening development in clinical applications
title_sort 3d tumor cultures for drug resistance and screening development in clinical applications
topic Cancer resistance
3D tumor culture model
Organoids
Tumor microenvironment
Drug screening
url https://doi.org/10.1186/s12943-025-02281-2
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AT linazhao 3dtumorculturesfordrugresistanceandscreeningdevelopmentinclinicalapplications
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