Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota

Chondroitin sulfate (CS) is one of the main bioactive compounds in animal cartilage. In this study, the anti-inflammatory activity of sturgeon-derived chondroitin sulfate (SCS) was evaluated in the dextran sulfate sodium (DSS)-induced BALB/c mice model. Orally administration of SCS significantly all...

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Main Authors: Ruiyun Wu, Zixin Han, Zhenyu Wang, Pinglan Li, Nan Shang
Format: Article
Language:English
Published: Tsinghua University Press 2025-04-01
Series:Food Science and Human Wellness
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Online Access:https://www.sciopen.com/article/10.26599/FSHW.2024.9250227
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author Ruiyun Wu
Zixin Han
Zhenyu Wang
Pinglan Li
Nan Shang
author_facet Ruiyun Wu
Zixin Han
Zhenyu Wang
Pinglan Li
Nan Shang
author_sort Ruiyun Wu
collection DOAJ
description Chondroitin sulfate (CS) is one of the main bioactive compounds in animal cartilage. In this study, the anti-inflammatory activity of sturgeon-derived chondroitin sulfate (SCS) was evaluated in the dextran sulfate sodium (DSS)-induced BALB/c mice model. Orally administration of SCS significantly alleviated the DSS-induced colitis symptoms, including the reduction of crypt depth, inhibition of the abnormal crypt foci formation, down-regulation of the proinflammatory biomarkers (NO, interleukin (IL)-6, IL-1β and tumor necrosis factor-α) and up-regulation of the anti-inflammatory biomarkers (IL-10 and IL-4). The gut microbiota analysis revealed that SCS alters the intestinal microbiota composition in colitis mice, especially the increase of the relative abundance of Ruminococcaceae and Lachnospiraceae. This alternation further induced primary bile acids convert into secondary bile acids. With SCS administration, the levels of deoxycholic acid (DCA) and litho cholic acid (LCA) were increased by 1.5- and 2.5-fold, respectively. The stimulated secretion of DCA and LCA showed further activation of the NF-κB signaling pathway, thereby suppressing the inflammatory response and attenuating inflammatory bowel disease (IBD) in mice. This study provided a valuable strategy for colitis prevention and treatment with sturgeon cartilage by-products.
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institution Kabale University
issn 2097-0765
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publishDate 2025-04-01
publisher Tsinghua University Press
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series Food Science and Human Wellness
spelling doaj-art-e9443d9515a940328f7c67dffb724d812025-08-20T03:42:40ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-04-01144925022710.26599/FSHW.2024.9250227Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiotaRuiyun Wu0Zixin Han1Zhenyu Wang2Pinglan Li3Nan Shang4Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Integrated Laboratory of Processing Technology for Chinese Meat and Dish Products, Ministry of Agriculture and Rural Affairs, Beijing 100193, ChinaCollage of Engineering, China Agricultural University, Beijing 100083, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Integrated Laboratory of Processing Technology for Chinese Meat and Dish Products, Ministry of Agriculture and Rural Affairs, Beijing 100193, ChinaCollage of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollage of Engineering, China Agricultural University, Beijing 100083, ChinaChondroitin sulfate (CS) is one of the main bioactive compounds in animal cartilage. In this study, the anti-inflammatory activity of sturgeon-derived chondroitin sulfate (SCS) was evaluated in the dextran sulfate sodium (DSS)-induced BALB/c mice model. Orally administration of SCS significantly alleviated the DSS-induced colitis symptoms, including the reduction of crypt depth, inhibition of the abnormal crypt foci formation, down-regulation of the proinflammatory biomarkers (NO, interleukin (IL)-6, IL-1β and tumor necrosis factor-α) and up-regulation of the anti-inflammatory biomarkers (IL-10 and IL-4). The gut microbiota analysis revealed that SCS alters the intestinal microbiota composition in colitis mice, especially the increase of the relative abundance of Ruminococcaceae and Lachnospiraceae. This alternation further induced primary bile acids convert into secondary bile acids. With SCS administration, the levels of deoxycholic acid (DCA) and litho cholic acid (LCA) were increased by 1.5- and 2.5-fold, respectively. The stimulated secretion of DCA and LCA showed further activation of the NF-κB signaling pathway, thereby suppressing the inflammatory response and attenuating inflammatory bowel disease (IBD) in mice. This study provided a valuable strategy for colitis prevention and treatment with sturgeon cartilage by-products.https://www.sciopen.com/article/10.26599/FSHW.2024.9250227sturgeon cartilagechondroitin sulfateanti-inflammatory activitydextran sulfate sodium-induced colitisgut microbiota
spellingShingle Ruiyun Wu
Zixin Han
Zhenyu Wang
Pinglan Li
Nan Shang
Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota
Food Science and Human Wellness
sturgeon cartilage
chondroitin sulfate
anti-inflammatory activity
dextran sulfate sodium-induced colitis
gut microbiota
title Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota
title_full Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota
title_fullStr Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota
title_full_unstemmed Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota
title_short Sturgeon cartilage-derived chondroitin sulfate exhibited anti-inflammatory activity against dextran sulfate sodium-induced colitis via modification of gut microbiota
title_sort sturgeon cartilage derived chondroitin sulfate exhibited anti inflammatory activity against dextran sulfate sodium induced colitis via modification of gut microbiota
topic sturgeon cartilage
chondroitin sulfate
anti-inflammatory activity
dextran sulfate sodium-induced colitis
gut microbiota
url https://www.sciopen.com/article/10.26599/FSHW.2024.9250227
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