Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage
<i>Panax ginseng</i> (Korean ginseng) is renowned for its health-promoting properties, attributed to its bioactive compounds, including saponins, polyphenols, and polysaccharides, which possess both antioxidant and anti-aging activities. This study investigated the anti-aging and anti-in...
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2024-11-01
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| author | Minseo Kang Somin Park So-Ri Son Yedam Noh Dae Sik Jang Sullim Lee |
| author_facet | Minseo Kang Somin Park So-Ri Son Yedam Noh Dae Sik Jang Sullim Lee |
| author_sort | Minseo Kang |
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| description | <i>Panax ginseng</i> (Korean ginseng) is renowned for its health-promoting properties, attributed to its bioactive compounds, including saponins, polyphenols, and polysaccharides, which possess both antioxidant and anti-aging activities. This study investigated the anti-aging and anti-inflammatory effects of compounds isolated from the hot water extract of fresh <i>P. ginseng</i> roots, evaluating their resistance to TNF-α/IFN-γ-induced skin cell damage. Among 14 compounds, ginsenoside Rf (compound <b>2</b>) showed significant multi-target effects. In NHDFs, ginsenoside Rf and others effectively reduced intracellular ROS, demonstrating strong antioxidant properties. Additionally, they inhibited MMP-1 expression, a key enzyme in collagen degradation, and promoted pro-collagen Type I synthesis, countering the negative effects of TNF-α and supporting skin health. Further analysis showed that ginsenoside Rf reduced the secretion of inflammatory cytokines like IL-1β and IL-6, exhibiting anti-inflammatory effects. It also promoted the expression of crucial skin barrier proteins, including LOR, AQP3, FLG, and KRT1 in TNF-α/IFN-γ-stimulated NHEKs, enhancing skin hydration and structural integrity. These results suggest that compounds from <i>P. ginseng</i> roots, especially ginsenoside Rf, hold promise as skincare agents targeting skin aging and inflammation. Future research should further explore their mechanisms and optimize their applications in dermatological treatments. |
| format | Article |
| id | doaj-art-e9422fc4e80342239993606d61263772 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-e9422fc4e80342239993606d612637722025-08-20T02:04:55ZengMDPI AGMolecules1420-30492024-11-012922547910.3390/molecules29225479Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell DamageMinseo Kang0Somin Park1So-Ri Son2Yedam Noh3Dae Sik Jang4Sullim Lee5Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Republic of Korea<i>Panax ginseng</i> (Korean ginseng) is renowned for its health-promoting properties, attributed to its bioactive compounds, including saponins, polyphenols, and polysaccharides, which possess both antioxidant and anti-aging activities. This study investigated the anti-aging and anti-inflammatory effects of compounds isolated from the hot water extract of fresh <i>P. ginseng</i> roots, evaluating their resistance to TNF-α/IFN-γ-induced skin cell damage. Among 14 compounds, ginsenoside Rf (compound <b>2</b>) showed significant multi-target effects. In NHDFs, ginsenoside Rf and others effectively reduced intracellular ROS, demonstrating strong antioxidant properties. Additionally, they inhibited MMP-1 expression, a key enzyme in collagen degradation, and promoted pro-collagen Type I synthesis, countering the negative effects of TNF-α and supporting skin health. Further analysis showed that ginsenoside Rf reduced the secretion of inflammatory cytokines like IL-1β and IL-6, exhibiting anti-inflammatory effects. It also promoted the expression of crucial skin barrier proteins, including LOR, AQP3, FLG, and KRT1 in TNF-α/IFN-γ-stimulated NHEKs, enhancing skin hydration and structural integrity. These results suggest that compounds from <i>P. ginseng</i> roots, especially ginsenoside Rf, hold promise as skincare agents targeting skin aging and inflammation. Future research should further explore their mechanisms and optimize their applications in dermatological treatments.https://www.mdpi.com/1420-3049/29/22/5479ginsenoside Rf<i>P. ginseng</i>anti-aginganti-inflammatoryROSMMP-1 |
| spellingShingle | Minseo Kang Somin Park So-Ri Son Yedam Noh Dae Sik Jang Sullim Lee Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage Molecules ginsenoside Rf <i>P. ginseng</i> anti-aging anti-inflammatory ROS MMP-1 |
| title | Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage |
| title_full | Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage |
| title_fullStr | Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage |
| title_full_unstemmed | Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage |
| title_short | Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh <i>Panax ginseng</i> Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage |
| title_sort | anti aging and anti inflammatory effects of compounds from fresh i panax ginseng i roots a study on tnf α ifn γ induced skin cell damage |
| topic | ginsenoside Rf <i>P. ginseng</i> anti-aging anti-inflammatory ROS MMP-1 |
| url | https://www.mdpi.com/1420-3049/29/22/5479 |
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