A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice
The development of standardised, reproducible preclinical models is essential for advancing pleural mesothelioma (PM) research. Here, we present a simple and reliable minimally invasive transthoracic intrapleural injection technique that could improve the efficiency of orthotopic PM model generation...
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MDPI AG
2025-05-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/8/3/55 |
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| author | Sophie Rovers Pooyeh Farahmand Dana Liu Louize Brants Christophe Hermans Dieter Peeters Danielle McKinven Jennifer Doig Filip Lardon Jan van Meerbeeck Elly Marcq Daniel J. Murphy Evelien Smits |
| author_facet | Sophie Rovers Pooyeh Farahmand Dana Liu Louize Brants Christophe Hermans Dieter Peeters Danielle McKinven Jennifer Doig Filip Lardon Jan van Meerbeeck Elly Marcq Daniel J. Murphy Evelien Smits |
| author_sort | Sophie Rovers |
| collection | DOAJ |
| description | The development of standardised, reproducible preclinical models is essential for advancing pleural mesothelioma (PM) research. Here, we present a simple and reliable minimally invasive transthoracic intrapleural injection technique that could improve the efficiency of orthotopic PM model generation. By incorporating a simple needle sleeve to control the injection depth, this method eliminates the need for surgery or general anaesthesia, reducing technical complexity and animal stress while ensuring precise delivery into the pleural cavity. We demonstrate the effectiveness of this approach by achieving a 100% tumour engraftment rate following the injection of AE17 tumour cells. Additionally, this technique has been successfully used for asbestos fibre injection in mesothelioma models, highlighting its versatility. By providing a more accessible, standardised alternative to existing methods, this protocol improves the reliability of PM models and facilitates broader adoption by researchers, including those with limited experience in invasive procedures. |
| format | Article |
| id | doaj-art-e940677659294477bd7ada508f841b34 |
| institution | Kabale University |
| issn | 2409-9279 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj-art-e940677659294477bd7ada508f841b342025-08-20T03:27:32ZengMDPI AGMethods and Protocols2409-92792025-05-01835510.3390/mps8030055A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in MiceSophie Rovers0Pooyeh Farahmand1Dana Liu2Louize Brants3Christophe Hermans4Dieter Peeters5Danielle McKinven6Jennifer Doig7Filip Lardon8Jan van Meerbeeck9Elly Marcq10Daniel J. Murphy11Evelien Smits12Center for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, BelgiumSchool of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKCenter for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, BelgiumCenter for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, BelgiumCenter for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, BelgiumDepartment of Pathology, Antwerp University Hospital, 2650 Edegem, BelgiumSchool of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKSchool of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKCenter for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, BelgiumMultidisciplinary Oncological Center Antwerp (MOCA), Antwerp University Hospital, 2650 Edegem, BelgiumLaboratory of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, 9052 Gent, BelgiumSchool of Cancer Sciences, University of Glasgow, Glasgow G61 1BD, UKCenter for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, BelgiumThe development of standardised, reproducible preclinical models is essential for advancing pleural mesothelioma (PM) research. Here, we present a simple and reliable minimally invasive transthoracic intrapleural injection technique that could improve the efficiency of orthotopic PM model generation. By incorporating a simple needle sleeve to control the injection depth, this method eliminates the need for surgery or general anaesthesia, reducing technical complexity and animal stress while ensuring precise delivery into the pleural cavity. We demonstrate the effectiveness of this approach by achieving a 100% tumour engraftment rate following the injection of AE17 tumour cells. Additionally, this technique has been successfully used for asbestos fibre injection in mesothelioma models, highlighting its versatility. By providing a more accessible, standardised alternative to existing methods, this protocol improves the reliability of PM models and facilitates broader adoption by researchers, including those with limited experience in invasive procedures.https://www.mdpi.com/2409-9279/8/3/55orthotopic modelsintrapleural injectionpleural mesotheliomatransthoracic injectionminimally invasive methodspreclinical models |
| spellingShingle | Sophie Rovers Pooyeh Farahmand Dana Liu Louize Brants Christophe Hermans Dieter Peeters Danielle McKinven Jennifer Doig Filip Lardon Jan van Meerbeeck Elly Marcq Daniel J. Murphy Evelien Smits A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice Methods and Protocols orthotopic models intrapleural injection pleural mesothelioma transthoracic injection minimally invasive methods preclinical models |
| title | A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice |
| title_full | A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice |
| title_fullStr | A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice |
| title_full_unstemmed | A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice |
| title_short | A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice |
| title_sort | minimally invasive transthoracic injection technique for reproducible intrapleural delivery in mice |
| topic | orthotopic models intrapleural injection pleural mesothelioma transthoracic injection minimally invasive methods preclinical models |
| url | https://www.mdpi.com/2409-9279/8/3/55 |
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