Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice
The increasing dietary demand for red swamp crayfish (Procambarus clarkii) in China has resulted in a significant rise in the quantity of its by-products, which are currently underutilized or discarded. Chitosan, a widely used plant immune inducer, is one of the major components found in red swamp c...
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Elsevier
2024-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893924001518 |
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| author | Lirong Wei Yiqiong Sun Yingqiao Wan Shangyu Gao Sun Tae Kim Ravi Gupta Yiming Wang |
| author_facet | Lirong Wei Yiqiong Sun Yingqiao Wan Shangyu Gao Sun Tae Kim Ravi Gupta Yiming Wang |
| author_sort | Lirong Wei |
| collection | DOAJ |
| description | The increasing dietary demand for red swamp crayfish (Procambarus clarkii) in China has resulted in a significant rise in the quantity of its by-products, which are currently underutilized or discarded. Chitosan, a widely used plant immune inducer, is one of the major components found in red swamp crayfish shells (RSCS). However, the utilization of chitosan derived from RSCS in agriculture is hindered by the lack of an efficient microbial fermentation-based production system. To address this issue, this study aims to establish a chitosan fermentation procedure by using RSCS as substrate. The efficiency by using Bacillus followed with Acetobacter for a continuous co-fermentation tested. Bacillus NJ-B2 or NJ-B3 with Acetobacter NJ-A1 demonstrated the highest co-fermentation efficacy, achieving 95 % deproteinization and 90 % demineralization of RSCS, respectively. The extracted chitosan was characterized by fourier transform infrared and scanning electron microscopy. Notably, the application of this RSCS-derived chitosan significantly improves rice resistance against Xanthomonas oryzae pv. oryzae, Magnaporthe oryzae, and Ustilaginoidea virens. These findings collectively indicate that this established method can effectively produce chitosan from RSCS via microbial fermentation, providing an environmentally friendly way for plant protection. |
| format | Article |
| id | doaj-art-e93c7cb7f5c84817934fde059b41bf73 |
| institution | OA Journals |
| issn | 2666-8939 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-e93c7cb7f5c84817934fde059b41bf732025-08-20T02:38:15ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392024-12-01810057110.1016/j.carpta.2024.100571Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in riceLirong Wei0Yiqiong Sun1Yingqiao Wan2Shangyu Gao3Sun Tae Kim4Ravi Gupta5Yiming Wang6Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Plant Bioscience, Pusan National University, Miryang 50463, South KoreaCollege of General Education, Kookmin University, Seoul 02707, South KoreaDepartment of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author.The increasing dietary demand for red swamp crayfish (Procambarus clarkii) in China has resulted in a significant rise in the quantity of its by-products, which are currently underutilized or discarded. Chitosan, a widely used plant immune inducer, is one of the major components found in red swamp crayfish shells (RSCS). However, the utilization of chitosan derived from RSCS in agriculture is hindered by the lack of an efficient microbial fermentation-based production system. To address this issue, this study aims to establish a chitosan fermentation procedure by using RSCS as substrate. The efficiency by using Bacillus followed with Acetobacter for a continuous co-fermentation tested. Bacillus NJ-B2 or NJ-B3 with Acetobacter NJ-A1 demonstrated the highest co-fermentation efficacy, achieving 95 % deproteinization and 90 % demineralization of RSCS, respectively. The extracted chitosan was characterized by fourier transform infrared and scanning electron microscopy. Notably, the application of this RSCS-derived chitosan significantly improves rice resistance against Xanthomonas oryzae pv. oryzae, Magnaporthe oryzae, and Ustilaginoidea virens. These findings collectively indicate that this established method can effectively produce chitosan from RSCS via microbial fermentation, providing an environmentally friendly way for plant protection.http://www.sciencedirect.com/science/article/pii/S2666893924001518ChitosanBacillusAcetobacterImmune activationRicePathogens |
| spellingShingle | Lirong Wei Yiqiong Sun Yingqiao Wan Shangyu Gao Sun Tae Kim Ravi Gupta Yiming Wang Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice Carbohydrate Polymer Technologies and Applications Chitosan Bacillus Acetobacter Immune activation Rice Pathogens |
| title | Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice |
| title_full | Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice |
| title_fullStr | Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice |
| title_full_unstemmed | Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice |
| title_short | Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice |
| title_sort | bacterial co fermentation mediated synthesis of chitosan from procambarus clarkii enhances disease resistance in rice |
| topic | Chitosan Bacillus Acetobacter Immune activation Rice Pathogens |
| url | http://www.sciencedirect.com/science/article/pii/S2666893924001518 |
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