Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis
Natural polymers have attracted considerable attention in recent decades among scientists due to their potential therapeutic uses, particularly as antimicrobial and antitumor agents. In this research, novel EPSs were extracted from garlic rhizosphere bacteria. The antibacterial and antitumor activit...
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Elsevier
2024-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266689392400149X |
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| author | Shimaa K. Ali F.M Mohamed Ahmed H. El-Ghorab Eman A. Hamed Moustafa A. Aboel-Ainin Mohamed A. Abdelgawad Khaled El‐Adl Hussein S. Mohamed |
| author_facet | Shimaa K. Ali F.M Mohamed Ahmed H. El-Ghorab Eman A. Hamed Moustafa A. Aboel-Ainin Mohamed A. Abdelgawad Khaled El‐Adl Hussein S. Mohamed |
| author_sort | Shimaa K. Ali |
| collection | DOAJ |
| description | Natural polymers have attracted considerable attention in recent decades among scientists due to their potential therapeutic uses, particularly as antimicrobial and antitumor agents. In this research, novel EPSs were extracted from garlic rhizosphere bacteria. The antibacterial and antitumor activities of the polymer were evaluated through biological assays. The antibacterial activity was tested against gram-positive microorganisms (such as Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus) and gram-negative organisms (such as Shigella sonnei and Escherichia coli). The most significant inhibition zone was observed with Listeria monocytogenes and S. typhi, measuring 35 mm, while the most miniature antibacterial effect was seen with Staphylococcus aureus at 23.67 mm.Furthermore, the inhibitory effect of the crude polymer was assessed using a broth medium with two strains of E. coli and Bacillus cereus. Electron microscope images displayed varying degrees of damage to bacterial cells in the treated broth. The antitumor activity was determined using the MTT test on colon carcinoma cells (HCT-116), hepatocellular carcinoma cells (HepG-2), and CaCO2 (intestinal carcinoma cells), with IC50 values of 188.86±6.17 µg/mL, 221.66±8.02 µg/mL, and 203.65±7.43 µg/mL, respectively, after 48 h. The bacteria responsible for polymer production were isolated from garlic plant rhizospheres and identified as Erwinia gerundensis CCASU-2024–69 through 16S rRNA sequencing. FTIR and NMR techniques determined the crude EPS's main components and functional groups, including carbonyl, carboxylic, methylene, and silanol. GC–MS analysis revealed 34 bioactive compounds, while HPLC analysis indicated that the EPS was a hetero-monosaccharide consisting of d-xylose, d-glucose, l-arabinose, ribose, and d-mannose. This research study represents the initial exploration into the exopolysaccharide derived from Erwinia gerundensis. To assess the interaction between the exopolysaccharide and the active sites of Bacillus cereus and E. coli, molecular docking experiments were conducted using five monosaccharides: d-xylose, d-glucose, l-arabinose, ribose, and d-mannose. The data obtained from the molecular docking analysis strongly correlates with the findings from biological studies.Furthermore, these highly active compounds exhibit a favorable proposed ADMET profile. This particular exopolysaccharide shows potential as a natural antibiotic and holds promise in treating gastrointestinal cancer. A comprehensive assessment of laboratory animals is essential before its potential use as a prebiotic in nutrition. |
| format | Article |
| id | doaj-art-e9391de5a25b48d78e8fb04ef0f4cba4 |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-e9391de5a25b48d78e8fb04ef0f4cba42025-08-20T02:50:16ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392024-12-01810056910.1016/j.carpta.2024.100569Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensisShimaa K. Ali0F.M Mohamed1Ahmed H. El-Ghorab2Eman A. Hamed3Moustafa A. Aboel-Ainin4Mohamed A. Abdelgawad5Khaled El‐Adl6Hussein S. Mohamed7Microbiology Department, Faculty of Agriculture, Beni-Suef University, 62511, EgyptHydrogeology and Environment Department, Faculty of Earth Sciences, Beni-Suef University, P.O 62521 EgyptDepartment of Chemistry, College of Science, Jouf University, Sakaka, Saudi Arabia; Corresponding authors.Biochemistry Department, Faculty of Agriculture, Beni-Suef University, 62511 EgyptBiochemistry Department, Faculty of Agriculture, Beni-Suef University, 62511 EgyptDepartment of pharmaceutical chemistry, college of pharmacy, Jouf University, Sakaka 72341, Aljouf, Saudi ArabiaChemistry Department, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo, Egypt; Pharmaceutical Medicinal Chemistry and Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City 11884, Cairo, EgyptChemistry of medicinal and aromatic plants department, Research Institute of Medicinal and Aromatic Plants (RIMAP), Beni-Suef University, 62511 Egypt; Corresponding authors.Natural polymers have attracted considerable attention in recent decades among scientists due to their potential therapeutic uses, particularly as antimicrobial and antitumor agents. In this research, novel EPSs were extracted from garlic rhizosphere bacteria. The antibacterial and antitumor activities of the polymer were evaluated through biological assays. The antibacterial activity was tested against gram-positive microorganisms (such as Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus) and gram-negative organisms (such as Shigella sonnei and Escherichia coli). The most significant inhibition zone was observed with Listeria monocytogenes and S. typhi, measuring 35 mm, while the most miniature antibacterial effect was seen with Staphylococcus aureus at 23.67 mm.Furthermore, the inhibitory effect of the crude polymer was assessed using a broth medium with two strains of E. coli and Bacillus cereus. Electron microscope images displayed varying degrees of damage to bacterial cells in the treated broth. The antitumor activity was determined using the MTT test on colon carcinoma cells (HCT-116), hepatocellular carcinoma cells (HepG-2), and CaCO2 (intestinal carcinoma cells), with IC50 values of 188.86±6.17 µg/mL, 221.66±8.02 µg/mL, and 203.65±7.43 µg/mL, respectively, after 48 h. The bacteria responsible for polymer production were isolated from garlic plant rhizospheres and identified as Erwinia gerundensis CCASU-2024–69 through 16S rRNA sequencing. FTIR and NMR techniques determined the crude EPS's main components and functional groups, including carbonyl, carboxylic, methylene, and silanol. GC–MS analysis revealed 34 bioactive compounds, while HPLC analysis indicated that the EPS was a hetero-monosaccharide consisting of d-xylose, d-glucose, l-arabinose, ribose, and d-mannose. This research study represents the initial exploration into the exopolysaccharide derived from Erwinia gerundensis. To assess the interaction between the exopolysaccharide and the active sites of Bacillus cereus and E. coli, molecular docking experiments were conducted using five monosaccharides: d-xylose, d-glucose, l-arabinose, ribose, and d-mannose. The data obtained from the molecular docking analysis strongly correlates with the findings from biological studies.Furthermore, these highly active compounds exhibit a favorable proposed ADMET profile. This particular exopolysaccharide shows potential as a natural antibiotic and holds promise in treating gastrointestinal cancer. A comprehensive assessment of laboratory animals is essential before its potential use as a prebiotic in nutrition.http://www.sciencedirect.com/science/article/pii/S266689392400149XAntibacterialAntitumorExopolymerErwinia gerundensisHPLCGC mass |
| spellingShingle | Shimaa K. Ali F.M Mohamed Ahmed H. El-Ghorab Eman A. Hamed Moustafa A. Aboel-Ainin Mohamed A. Abdelgawad Khaled El‐Adl Hussein S. Mohamed Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis Carbohydrate Polymer Technologies and Applications Antibacterial Antitumor Exopolymer Erwinia gerundensis HPLC GC mass |
| title | Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis |
| title_full | Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis |
| title_fullStr | Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis |
| title_full_unstemmed | Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis |
| title_short | Biological activity and chemical characteristics studies of new oligomannose produced by Erwinia gerundensis |
| title_sort | biological activity and chemical characteristics studies of new oligomannose produced by erwinia gerundensis |
| topic | Antibacterial Antitumor Exopolymer Erwinia gerundensis HPLC GC mass |
| url | http://www.sciencedirect.com/science/article/pii/S266689392400149X |
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