The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains
Considering the importance of macrophages as the first line of defense against fungal infection and the different roles played by the two M1- and M2-like polarized macrophages, we decided to evaluate the effects of Paracoccidioides brasiliensis infection on GM-CSF- and M-CSF-induced bone marrow-deri...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/605450 |
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| author | Calliandra de Souza Silva Aldo Henrique Tavares Marcio Sousa Jeronimo Yasmin Soares de Lima Lorena da Silveira Derengowski Anamélia Lorenzetti Bocca Ildinete Silva-Pereira |
| author_facet | Calliandra de Souza Silva Aldo Henrique Tavares Marcio Sousa Jeronimo Yasmin Soares de Lima Lorena da Silveira Derengowski Anamélia Lorenzetti Bocca Ildinete Silva-Pereira |
| author_sort | Calliandra de Souza Silva |
| collection | DOAJ |
| description | Considering the importance of macrophages as the first line of defense against fungal infection and the different roles played by the two M1- and M2-like polarized macrophages, we decided to evaluate the effects of Paracoccidioides brasiliensis infection on GM-CSF- and M-CSF-induced bone marrow-derived macrophages (BMM) from the A/J and B10.A mouse strains, an established model of resistance/susceptibility to PCM, respectively. Upon differentiation, the generated GM- or M-BMMs were characterized by morphological analyses, gene expression profiles, and cytokines production. Our main results demonstrate that GM-BMMs derived from A/J and B.10 produced high levels of pro- and anti-inflammatory cytokines that may contribute to generate an unbalanced early immune response. In accordance with the literature, the B10.A susceptible mice lineage has an innate tendency to polarize into M1-like phenotype, whereas the opposite phenotype occurs in A/J resistance mice. In this context, our data support that susceptibility and resistance are strongly correlated with M1 and M2 polarization, respectively. |
| format | Article |
| id | doaj-art-e93200c8b20043bd8bf41a6e4342253a |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e93200c8b20043bd8bf41a6e4342253a2025-08-20T02:21:53ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/605450605450The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice StrainsCalliandra de Souza Silva0Aldo Henrique Tavares1Marcio Sousa Jeronimo2Yasmin Soares de Lima3Lorena da Silveira Derengowski4Anamélia Lorenzetti Bocca5Ildinete Silva-Pereira6Laboratório de Biologia Molecular, CEL/IB, Campus Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, BrazilLaboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, BrazilLaboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, BrazilLaboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, BrazilLaboratório de Biologia Molecular, CEL/IB, Campus Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, BrazilLaboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, BrazilLaboratório de Biologia Molecular, CEL/IB, Campus Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, BrazilConsidering the importance of macrophages as the first line of defense against fungal infection and the different roles played by the two M1- and M2-like polarized macrophages, we decided to evaluate the effects of Paracoccidioides brasiliensis infection on GM-CSF- and M-CSF-induced bone marrow-derived macrophages (BMM) from the A/J and B10.A mouse strains, an established model of resistance/susceptibility to PCM, respectively. Upon differentiation, the generated GM- or M-BMMs were characterized by morphological analyses, gene expression profiles, and cytokines production. Our main results demonstrate that GM-BMMs derived from A/J and B.10 produced high levels of pro- and anti-inflammatory cytokines that may contribute to generate an unbalanced early immune response. In accordance with the literature, the B10.A susceptible mice lineage has an innate tendency to polarize into M1-like phenotype, whereas the opposite phenotype occurs in A/J resistance mice. In this context, our data support that susceptibility and resistance are strongly correlated with M1 and M2 polarization, respectively.http://dx.doi.org/10.1155/2015/605450 |
| spellingShingle | Calliandra de Souza Silva Aldo Henrique Tavares Marcio Sousa Jeronimo Yasmin Soares de Lima Lorena da Silveira Derengowski Anamélia Lorenzetti Bocca Ildinete Silva-Pereira The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains Mediators of Inflammation |
| title | The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains |
| title_full | The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains |
| title_fullStr | The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains |
| title_full_unstemmed | The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains |
| title_short | The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains |
| title_sort | effects of paracoccidioides brasiliensis infection on gm csf and m csf induced mouse bone marrow derived macrophage from resistant and susceptible mice strains |
| url | http://dx.doi.org/10.1155/2015/605450 |
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