Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking
Protein crystallogenesis represents a key step in X‐ray crystallography studies that employ co‐crystallization and ligand soaking for investigating ligand binding to proteins. Co‐crystallization is a method that enables the precise determination of binding positions, although it necessitates a signi...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13913 |
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| author | Barbora Kaščáková Anna Koutská Michaela Burdová Petra Havlíčková Ivana Kutá Smatanová |
| author_facet | Barbora Kaščáková Anna Koutská Michaela Burdová Petra Havlíčková Ivana Kutá Smatanová |
| author_sort | Barbora Kaščáková |
| collection | DOAJ |
| description | Protein crystallogenesis represents a key step in X‐ray crystallography studies that employ co‐crystallization and ligand soaking for investigating ligand binding to proteins. Co‐crystallization is a method that enables the precise determination of binding positions, although it necessitates a significant degree of optimization. The utilization of microseeding can facilitate a reduction in sample requirements and accelerate the co‐crystallization process. Ligand soaking is the preferred method due to its simplicity; however, it requires careful control of soaking conditions to ensure the successful integration of the ligands. This research protocol details the procedures for co‐crystallization and soaking to achieve protein–ligand complex formation, which is essential for advancing drug discovery. Additionally, a simple protocol for demonstrating soaking for educational purposes is described. |
| format | Article |
| id | doaj-art-e92cf4107b864c849b4f2a35626bb93d |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-e92cf4107b864c849b4f2a35626bb93d2025-08-20T01:55:37ZengWileyFEBS Open Bio2211-54632025-04-0115454255010.1002/2211-5463.13913Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soakingBarbora Kaščáková0Anna Koutská1Michaela Burdová2Petra Havlíčková3Ivana Kutá Smatanová4Department of Chemistry, Faculty of Science University of South Bohemia in České Budějovice Czech RepublicDepartment of Chemistry, Faculty of Science University of South Bohemia in České Budějovice Czech RepublicDepartment of Chemistry, Faculty of Science University of South Bohemia in České Budějovice Czech RepublicDepartment of Chemistry, Faculty of Science University of South Bohemia in České Budějovice Czech RepublicDepartment of Chemistry, Faculty of Science University of South Bohemia in České Budějovice Czech RepublicProtein crystallogenesis represents a key step in X‐ray crystallography studies that employ co‐crystallization and ligand soaking for investigating ligand binding to proteins. Co‐crystallization is a method that enables the precise determination of binding positions, although it necessitates a significant degree of optimization. The utilization of microseeding can facilitate a reduction in sample requirements and accelerate the co‐crystallization process. Ligand soaking is the preferred method due to its simplicity; however, it requires careful control of soaking conditions to ensure the successful integration of the ligands. This research protocol details the procedures for co‐crystallization and soaking to achieve protein–ligand complex formation, which is essential for advancing drug discovery. Additionally, a simple protocol for demonstrating soaking for educational purposes is described.https://doi.org/10.1002/2211-5463.13913advanced crystallizationco‐crystallizationcrystal soakingcrystallization protocolmicroseeding |
| spellingShingle | Barbora Kaščáková Anna Koutská Michaela Burdová Petra Havlíčková Ivana Kutá Smatanová Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking FEBS Open Bio advanced crystallization co‐crystallization crystal soaking crystallization protocol microseeding |
| title | Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking |
| title_full | Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking |
| title_fullStr | Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking |
| title_full_unstemmed | Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking |
| title_short | Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking |
| title_sort | revealing protein structures crystallization of protein ligand complexes co crystallization and crystal soaking |
| topic | advanced crystallization co‐crystallization crystal soaking crystallization protocol microseeding |
| url | https://doi.org/10.1002/2211-5463.13913 |
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