Purification and cytotoxic assays of four antimicrobial metabolites extracted from Actinomycetes of the soils of Menengai Crater, Kenya
The search for antibiotic metabolites is increasing due to drug resistance been witnessed today. This study was conceived to isolate, identify and compare the antimicrobial metabolites yield of four actinomycetes isolated from Menengai crater soil, Kenya. The actinomycetes were isolated using star...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2019-06-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_37208_c0f74577bd8ac488acd03e40bebe412f.pdf |
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| Summary: | The search for antibiotic metabolites is increasing due to drug resistance been witnessed today. This study
was conceived to isolate, identify and compare the antimicrobial metabolites yield of four actinomycetes isolated
from Menengai crater soil, Kenya. The actinomycetes were isolated using starch casein, Luria Bertani (M1) and
starch nitrate agar. The antimicrobial metabolites were extracted using standard techniques followed by
purification, test for antagonism against selected pathogenic microbes, and cytotoxicity assay using Brine shrimp
lethality test. Totally, 138 actinomycetes isolates were obtained from all the soil samples. Four isolates showed
the highest potent potential against selected bacterial and fungal pathogens. The selected actinomycetes were
coded; PAN 30, PAN 37, PAN 41, and PAN 154, and preserved at 4°C for further analysis. The difference in
yield of the antimicrobial metabolites between liquid and solid-state fermentation was statistically significant (P=
0.005). Zones of inhibition did not vary significantly (F = 6.6046, P = 0.001338). The minimum inhibitory
concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC)
of the antimicrobial metabolites extracted from PAN 30, PAN 37, PAN 41 and PAN 154 isolates were equal. The
Half Lethal Concentration (LC50) in (µg/ ml) for the antimicrobial metabolites extracted from the 4 isolates were;
PAN 30 (1.8168 ± 0.47), PAN 37 (3.4269 ± 0.56), PAN 41 (3.4269 ± 0.45), and PAN 154 (4.9397 ± 0.41). The
four actinomycetes produced bioactive metabolites. Solid-state fermentation was superior to liquid-state
fermentation in recovering the antimicrobial metabolites from the actinomycetes isolates. Molecular identification
of the selected isolates needs to be carried out in a future study. Moreover, there is a need to determine the
structure of the antimicrobials using nuclear magnetic resonance. |
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| ISSN: | 2537-0286 2537-0294 |