A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens
The diagnosis and confirmation of oral SCC (squamous cell carcinoma) is still dependent on histopathology report in spite of development of radiological investigations. It is, thus important to understand the underlying molecular mechanisms and how the alterations in metabolic pathways effect the tu...
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Elsevier
2018-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426817301082 |
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| author | Rohit Khanna Kapila Kumar Raja Roy |
| author_facet | Rohit Khanna Kapila Kumar Raja Roy |
| author_sort | Rohit Khanna |
| collection | DOAJ |
| description | The diagnosis and confirmation of oral SCC (squamous cell carcinoma) is still dependent on histopathology report in spite of development of radiological investigations. It is, thus important to understand the underlying molecular mechanisms and how the alterations in metabolic pathways effect the tumor development and progression. The simultaneous and comprehensive information about the presence and absence of small molecule metabolites and their relative concentrations has been provided by 1H HR-MAS NMR spectroscopy on tissue specimens. In this paper a unique case study was presented in order to correlate histological and NMR spectroscopic findings. The patient’s initially lesion was found to be non-malignant in nature based on histological findings but its periodic localized recurrence even after laser ablation prompted us to perform HR-MAS based analysis and its role in identifying the metabolic alterations in known pathways occurring during its progressions. Thus it was confirmed after analysis that HR-MAS NMR can also be used as an analytical tool which is reliable in order to distinguish between malignant and non-malignant tissues, in combination with histopathology. |
| format | Article |
| id | doaj-art-e92b84c9fdcb447391047f4320db01c4 |
| institution | DOAJ |
| issn | 2212-4268 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-e92b84c9fdcb447391047f4320db01c42025-08-20T02:50:29ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682018-01-0181687210.1016/j.jobcr.2017.09.011A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimensRohit Khanna0Kapila Kumar1Raja Roy2Department of Orthodontics and Dentofacial Orthopaedics, Babu Banarsi Das College of Dental Sciences, Lucknow, IndiaDepartment of Oral & Maxillofacial Surgery, King George’s Medical College, Lucknow, IndiaCentre of Biomedical Research, formerly Centre of Biomedical Magnetic Resonance, Raebareilly Road, Lucknow, India; Corresponding author.The diagnosis and confirmation of oral SCC (squamous cell carcinoma) is still dependent on histopathology report in spite of development of radiological investigations. It is, thus important to understand the underlying molecular mechanisms and how the alterations in metabolic pathways effect the tumor development and progression. The simultaneous and comprehensive information about the presence and absence of small molecule metabolites and their relative concentrations has been provided by 1H HR-MAS NMR spectroscopy on tissue specimens. In this paper a unique case study was presented in order to correlate histological and NMR spectroscopic findings. The patient’s initially lesion was found to be non-malignant in nature based on histological findings but its periodic localized recurrence even after laser ablation prompted us to perform HR-MAS based analysis and its role in identifying the metabolic alterations in known pathways occurring during its progressions. Thus it was confirmed after analysis that HR-MAS NMR can also be used as an analytical tool which is reliable in order to distinguish between malignant and non-malignant tissues, in combination with histopathology.http://www.sciencedirect.com/science/article/pii/S2212426817301082Oral squamous cell carcinoma1H HR-MAS NMR spectroscopyMetabolomicsHistopathology |
| spellingShingle | Rohit Khanna Kapila Kumar Raja Roy A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens Journal of Oral Biology and Craniofacial Research Oral squamous cell carcinoma 1H HR-MAS NMR spectroscopy Metabolomics Histopathology |
| title | A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens |
| title_full | A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens |
| title_fullStr | A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens |
| title_full_unstemmed | A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens |
| title_short | A case study of primary malignancy of buccal mucosa using proton HR-MAS NMR spectroscopy on tissue specimens |
| title_sort | case study of primary malignancy of buccal mucosa using proton hr mas nmr spectroscopy on tissue specimens |
| topic | Oral squamous cell carcinoma 1H HR-MAS NMR spectroscopy Metabolomics Histopathology |
| url | http://www.sciencedirect.com/science/article/pii/S2212426817301082 |
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