Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model
Introduction: Inflammatory bowel disease (IBD) is a group of chronic gastrointestinal disorders affecting millions worldwide. Several factors are involved in developing this disease, but gut microbiota is known to be one of the most critical factors. This study investigated the relationship betwe...
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Pasteur Institute of Iran
2022-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| author | Afsaneh Salimi1 Mahdi Rohani1* Mohammad Reza Pourshafie1* |
| author_facet | Afsaneh Salimi1 Mahdi Rohani1* Mohammad Reza Pourshafie1* |
| author_sort | Afsaneh Salimi1 |
| collection | DOAJ |
| description | Introduction: Inflammatory bowel disease (IBD) is a group of chronic
gastrointestinal disorders affecting millions worldwide. Several factors are
involved in developing this disease, but gut microbiota is known to be one of
the most critical factors. This study investigated the relationship between gut
microbiota and IBD in a mouse model. Methods: In this study, two methods
were used: chemical induction with dextran sulfate sodium (DSS) and biological
induction with stool from a human with IBD (fecal microbiota transplantation)
to induce inflammation in the gut of mice. The gut microbiota populations in
both groups were studied using real-time PCR. In addition, the serum levels of
inflammatory cytokines and the colon tissues of the mice were analyzed.
Results: The pathological results showed that the colon tissue in the FMT group
had inflammatory changes as in the DSS group. The changes in the gut
microbiota population in both FMT and DSS groups on the last day of the study
also showed a similar pattern. Interleukin-1 and IL-6 also increased in the FMT
and DSS groups compared to the control group. Conclusion: Our results
showed a mutual relationship between gut microbiota and inflammatory
diseases and that gut microbiota was not only the cause of IBD but may also be
a consequence of this disease. In fact, by chemically inducing inflammation, the
gut microbiota was altered. On the other hand, performing FMT from human
stool with IBD altered the gut microbiota of mice and induced inflammatory
disease in the mouse model. |
| format | Article |
| id | doaj-art-e91fd6ec09164928b1ad844812f446d4 |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-e91fd6ec09164928b1ad844812f446d42025-08-20T02:28:41ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302022-09-0110312212810.52547/JoMMID.10.3.122Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse ModelAfsaneh Salimi10https://orcid.org/0000-0002-7669-9343Mahdi Rohani1*1https://orcid.org/0000-0001-8956-5072Mohammad Reza Pourshafie1*2https://orcid.org/0000-0002-6226-70641Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran1Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran1Department of Bacteriology, Pasteur Institute of Iran, Tehran, IranIntroduction: Inflammatory bowel disease (IBD) is a group of chronic gastrointestinal disorders affecting millions worldwide. Several factors are involved in developing this disease, but gut microbiota is known to be one of the most critical factors. This study investigated the relationship between gut microbiota and IBD in a mouse model. Methods: In this study, two methods were used: chemical induction with dextran sulfate sodium (DSS) and biological induction with stool from a human with IBD (fecal microbiota transplantation) to induce inflammation in the gut of mice. The gut microbiota populations in both groups were studied using real-time PCR. In addition, the serum levels of inflammatory cytokines and the colon tissues of the mice were analyzed. Results: The pathological results showed that the colon tissue in the FMT group had inflammatory changes as in the DSS group. The changes in the gut microbiota population in both FMT and DSS groups on the last day of the study also showed a similar pattern. Interleukin-1 and IL-6 also increased in the FMT and DSS groups compared to the control group. Conclusion: Our results showed a mutual relationship between gut microbiota and inflammatory diseases and that gut microbiota was not only the cause of IBD but may also be a consequence of this disease. In fact, by chemically inducing inflammation, the gut microbiota was altered. On the other hand, performing FMT from human stool with IBD altered the gut microbiota of mice and induced inflammatory disease in the mouse model.inflammatory bowel diseasegut microbiotafecal microbiota transplantationdextran sulfate sodium |
| spellingShingle | Afsaneh Salimi1 Mahdi Rohani1* Mohammad Reza Pourshafie1* Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model Journal of Medical Microbiology and Infectious Diseases inflammatory bowel disease gut microbiota fecal microbiota transplantation dextran sulfate sodium |
| title | Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model |
| title_full | Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model |
| title_fullStr | Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model |
| title_full_unstemmed | Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model |
| title_short | Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model |
| title_sort | investigating the relation between the gut microbiota and inflammatory bowel disease in a mouse model |
| topic | inflammatory bowel disease gut microbiota fecal microbiota transplantation dextran sulfate sodium |
| work_keys_str_mv | AT afsanehsalimi1 investigatingtherelationbetweenthegutmicrobiotaandinflammatoryboweldiseaseinamousemodel AT mahdirohani1 investigatingtherelationbetweenthegutmicrobiotaandinflammatoryboweldiseaseinamousemodel AT mohammadrezapourshafie1 investigatingtherelationbetweenthegutmicrobiotaandinflammatoryboweldiseaseinamousemodel |