Investigating the Relation between the Gut Microbiota and Inflammatory Bowel Disease in a Mouse Model
Introduction: Inflammatory bowel disease (IBD) is a group of chronic gastrointestinal disorders affecting millions worldwide. Several factors are involved in developing this disease, but gut microbiota is known to be one of the most critical factors. This study investigated the relationship betwe...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2022-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
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| Summary: | Introduction: Inflammatory bowel disease (IBD) is a group of chronic
gastrointestinal disorders affecting millions worldwide. Several factors are
involved in developing this disease, but gut microbiota is known to be one of
the most critical factors. This study investigated the relationship between gut
microbiota and IBD in a mouse model. Methods: In this study, two methods
were used: chemical induction with dextran sulfate sodium (DSS) and biological
induction with stool from a human with IBD (fecal microbiota transplantation)
to induce inflammation in the gut of mice. The gut microbiota populations in
both groups were studied using real-time PCR. In addition, the serum levels of
inflammatory cytokines and the colon tissues of the mice were analyzed.
Results: The pathological results showed that the colon tissue in the FMT group
had inflammatory changes as in the DSS group. The changes in the gut
microbiota population in both FMT and DSS groups on the last day of the study
also showed a similar pattern. Interleukin-1 and IL-6 also increased in the FMT
and DSS groups compared to the control group. Conclusion: Our results
showed a mutual relationship between gut microbiota and inflammatory
diseases and that gut microbiota was not only the cause of IBD but may also be
a consequence of this disease. In fact, by chemically inducing inflammation, the
gut microbiota was altered. On the other hand, performing FMT from human
stool with IBD altered the gut microbiota of mice and induced inflammatory
disease in the mouse model. |
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| ISSN: | 2345-5349 2345-5330 |