Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment
We investigated changes in PA levels by the treatment of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in ischemic stroke in rat brain model and in cultured neuronal SH-SY5Y cells exposed to oxygen-glucose deprivation (OGD). In ischemic rat model, transient middle cerebral artery occlu...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4612531 |
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| author | Tae Hwan Shin Geetika Phukan Jeom Soon Shim Duc-Toan Nguyen Yongman Kim Justin D. Oh-Lee Hyeon-Seong Lee Man Jeong Paik Gwang Lee |
| author_facet | Tae Hwan Shin Geetika Phukan Jeom Soon Shim Duc-Toan Nguyen Yongman Kim Justin D. Oh-Lee Hyeon-Seong Lee Man Jeong Paik Gwang Lee |
| author_sort | Tae Hwan Shin |
| collection | DOAJ |
| description | We investigated changes in PA levels by the treatment of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in ischemic stroke in rat brain model and in cultured neuronal SH-SY5Y cells exposed to oxygen-glucose deprivation (OGD). In ischemic rat model, transient middle cerebral artery occlusion (MCAo) was performed for 2 h, followed by intravenous transplantation of hBM-MSCs or phosphate-buffered saline (PBS) the day following MCAo. Metabolic profiling analysis of PAs was examined in brains from three groups: control rats, PBS-treated MCAo rats (MCAo), and hBM-MSCs-treated MCAo rats (MCAo + hBM-MSCs). In ischemic cell model, SH-SY5Y cells were exposed to OGD for 24 h, treated with hBM-MSCs (OGD + hBM-MSCs) prior to continued aerobic incubation, and then samples were collected after coculture for 72 h. In the in vivo MCAo ischemic model, levels of some PAs in brain samples of the MCAo and MCAo + hBM-MSCs groups were significantly different from those of the control group. In particular, putrescine, cadaverine, and spermidine in brain tissues of the MCAo + hBM-MSCs group were significantly reduced in comparison to those in the MCAo group. In the in vitro OGD system, N1-acetylspermidine, spermidine, N1-acetylspermine, and spermine in cells of the OGD + hBM-MSCs group were significantly reduced compared to those of OGD group. |
| format | Article |
| id | doaj-art-e91a286ae6e9422da2362a940652cc10 |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e91a286ae6e9422da2362a940652cc102025-08-20T03:21:09ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/46125314612531Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell TreatmentTae Hwan Shin0Geetika Phukan1Jeom Soon Shim2Duc-Toan Nguyen3Yongman Kim4Justin D. Oh-Lee5Hyeon-Seong Lee6Man Jeong Paik7Gwang Lee8Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Republic of KoreaDepartment of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of KoreaDepartment of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of KoreaNational Institute of Drug Quality Control, 48 Hai Ba Trung Street, Hanoi, VietnamPharmicell Co., Ltd., Seongnam 462-806, Republic of KoreaDepartment of Psychology, Central Michigan University, Health Professions Building, MI 48859, USADepartment of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of KoreaCollege of Pharmacy, Sunchon National University, Suncheon 540-950, Republic of KoreaDepartment of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 443-721, Republic of KoreaWe investigated changes in PA levels by the treatment of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) in ischemic stroke in rat brain model and in cultured neuronal SH-SY5Y cells exposed to oxygen-glucose deprivation (OGD). In ischemic rat model, transient middle cerebral artery occlusion (MCAo) was performed for 2 h, followed by intravenous transplantation of hBM-MSCs or phosphate-buffered saline (PBS) the day following MCAo. Metabolic profiling analysis of PAs was examined in brains from three groups: control rats, PBS-treated MCAo rats (MCAo), and hBM-MSCs-treated MCAo rats (MCAo + hBM-MSCs). In ischemic cell model, SH-SY5Y cells were exposed to OGD for 24 h, treated with hBM-MSCs (OGD + hBM-MSCs) prior to continued aerobic incubation, and then samples were collected after coculture for 72 h. In the in vivo MCAo ischemic model, levels of some PAs in brain samples of the MCAo and MCAo + hBM-MSCs groups were significantly different from those of the control group. In particular, putrescine, cadaverine, and spermidine in brain tissues of the MCAo + hBM-MSCs group were significantly reduced in comparison to those in the MCAo group. In the in vitro OGD system, N1-acetylspermidine, spermidine, N1-acetylspermine, and spermine in cells of the OGD + hBM-MSCs group were significantly reduced compared to those of OGD group.http://dx.doi.org/10.1155/2016/4612531 |
| spellingShingle | Tae Hwan Shin Geetika Phukan Jeom Soon Shim Duc-Toan Nguyen Yongman Kim Justin D. Oh-Lee Hyeon-Seong Lee Man Jeong Paik Gwang Lee Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment Stem Cells International |
| title | Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment |
| title_full | Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment |
| title_fullStr | Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment |
| title_full_unstemmed | Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment |
| title_short | Restoration of Polyamine Metabolic Patterns in In Vivo and In Vitro Model of Ischemic Stroke following Human Mesenchymal Stem Cell Treatment |
| title_sort | restoration of polyamine metabolic patterns in in vivo and in vitro model of ischemic stroke following human mesenchymal stem cell treatment |
| url | http://dx.doi.org/10.1155/2016/4612531 |
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