12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII

Abstract Aging poses significant challenges to cardiovascular health necessitating novel therapeutic approaches. This study investigates the potential of the brown adipose tissue (BAT) derived lipokine 12,13-diHOME to mitigate age-induced impairments in cardiovascular function. Analysis of human and...

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Main Authors: Shinsuke Nirengi, Benjamin Buck, Devleena Das, Carmem Peres Valgas da Silva, Jazmin Calyeca, Lisa A. Baer, Hsiang-Ling Huang, Pablo Vidal, Revati S. Dewal, Kelsey M. Pinckard, Elisa Félix-Soriano, Diego Hernandez-Saavedra, Andrew Gerea, Kavya Dathathreya, Silvia Duarte-Sanmiguel, Ty A. Saldana, Harrison L. Hookfin, Matthew W. Gorr, Valerie Bussberg, Juan J. Aristizabal-Henao, Michael A. Kiebish, Roeland J. W. Middelbeek, Laurie J. Goodyear, Paul M. Coen, Krishna Chinthalapudi, Loren E. Wold, Ana L. Mora, Thomas J. Hund, Daniel Gallego-Perez, Kristin I. Stanford
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-62474-7
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author Shinsuke Nirengi
Benjamin Buck
Devleena Das
Carmem Peres Valgas da Silva
Jazmin Calyeca
Lisa A. Baer
Hsiang-Ling Huang
Pablo Vidal
Revati S. Dewal
Kelsey M. Pinckard
Elisa Félix-Soriano
Diego Hernandez-Saavedra
Andrew Gerea
Kavya Dathathreya
Silvia Duarte-Sanmiguel
Ty A. Saldana
Harrison L. Hookfin
Matthew W. Gorr
Valerie Bussberg
Juan J. Aristizabal-Henao
Michael A. Kiebish
Roeland J. W. Middelbeek
Laurie J. Goodyear
Paul M. Coen
Krishna Chinthalapudi
Loren E. Wold
Ana L. Mora
Thomas J. Hund
Daniel Gallego-Perez
Kristin I. Stanford
author_facet Shinsuke Nirengi
Benjamin Buck
Devleena Das
Carmem Peres Valgas da Silva
Jazmin Calyeca
Lisa A. Baer
Hsiang-Ling Huang
Pablo Vidal
Revati S. Dewal
Kelsey M. Pinckard
Elisa Félix-Soriano
Diego Hernandez-Saavedra
Andrew Gerea
Kavya Dathathreya
Silvia Duarte-Sanmiguel
Ty A. Saldana
Harrison L. Hookfin
Matthew W. Gorr
Valerie Bussberg
Juan J. Aristizabal-Henao
Michael A. Kiebish
Roeland J. W. Middelbeek
Laurie J. Goodyear
Paul M. Coen
Krishna Chinthalapudi
Loren E. Wold
Ana L. Mora
Thomas J. Hund
Daniel Gallego-Perez
Kristin I. Stanford
author_sort Shinsuke Nirengi
collection DOAJ
description Abstract Aging poses significant challenges to cardiovascular health necessitating novel therapeutic approaches. This study investigates the potential of the brown adipose tissue (BAT) derived lipokine 12,13-diHOME to mitigate age-induced impairments in cardiovascular function. Analysis of human and rodent plasma signaling lipids reveals a decline in 12,13-diHOME levels with age. Transplantation of BAT or sustained upregulation of 12,13-diHOME effectively preserved cardiac function in aged male and female mice. Bulk RNA-Seq of hearts from aged mice reveals significant increases in pathways involved in ER stress and fibrosis which were partially attenuated by BAT transplantation or sustained upregulation of 12,13-diHOME. Mechanistically, in vivo and in vitro models demonstrate that 12,13-diHOME alleviated ER stress through CaMKII inhibition, particularly in males. These findings underscore 12,13-diHOME as a promising candidate for combating age-related cardiovascular dysfunction, offering insights into potential therapeutic strategies for addressing cardiovascular diseases in aging populations.
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spelling doaj-art-e914fa343a7c48d2affdd61a9017c9142025-08-20T04:03:03ZengNature PortfolioNature Communications2041-17232025-08-0116111510.1038/s41467-025-62474-712,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKIIShinsuke Nirengi0Benjamin Buck1Devleena Das2Carmem Peres Valgas da Silva3Jazmin Calyeca4Lisa A. Baer5Hsiang-Ling Huang6Pablo Vidal7Revati S. Dewal8Kelsey M. Pinckard9Elisa Félix-Soriano10Diego Hernandez-Saavedra11Andrew Gerea12Kavya Dathathreya13Silvia Duarte-Sanmiguel14Ty A. Saldana15Harrison L. Hookfin16Matthew W. Gorr17Valerie Bussberg18Juan J. Aristizabal-Henao19Michael A. Kiebish20Roeland J. W. Middelbeek21Laurie J. Goodyear22Paul M. Coen23Krishna Chinthalapudi24Loren E. Wold25Ana L. Mora26Thomas J. Hund27Daniel Gallego-Perez28Kristin I. Stanford29Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterBGPBioBGPBioBGPBioSection on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical SchoolSection on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical SchoolTranslational Research Institute for Metabolism and Diabetes, AdventHealthDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterDorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical CenterAbstract Aging poses significant challenges to cardiovascular health necessitating novel therapeutic approaches. This study investigates the potential of the brown adipose tissue (BAT) derived lipokine 12,13-diHOME to mitigate age-induced impairments in cardiovascular function. Analysis of human and rodent plasma signaling lipids reveals a decline in 12,13-diHOME levels with age. Transplantation of BAT or sustained upregulation of 12,13-diHOME effectively preserved cardiac function in aged male and female mice. Bulk RNA-Seq of hearts from aged mice reveals significant increases in pathways involved in ER stress and fibrosis which were partially attenuated by BAT transplantation or sustained upregulation of 12,13-diHOME. Mechanistically, in vivo and in vitro models demonstrate that 12,13-diHOME alleviated ER stress through CaMKII inhibition, particularly in males. These findings underscore 12,13-diHOME as a promising candidate for combating age-related cardiovascular dysfunction, offering insights into potential therapeutic strategies for addressing cardiovascular diseases in aging populations.https://doi.org/10.1038/s41467-025-62474-7
spellingShingle Shinsuke Nirengi
Benjamin Buck
Devleena Das
Carmem Peres Valgas da Silva
Jazmin Calyeca
Lisa A. Baer
Hsiang-Ling Huang
Pablo Vidal
Revati S. Dewal
Kelsey M. Pinckard
Elisa Félix-Soriano
Diego Hernandez-Saavedra
Andrew Gerea
Kavya Dathathreya
Silvia Duarte-Sanmiguel
Ty A. Saldana
Harrison L. Hookfin
Matthew W. Gorr
Valerie Bussberg
Juan J. Aristizabal-Henao
Michael A. Kiebish
Roeland J. W. Middelbeek
Laurie J. Goodyear
Paul M. Coen
Krishna Chinthalapudi
Loren E. Wold
Ana L. Mora
Thomas J. Hund
Daniel Gallego-Perez
Kristin I. Stanford
12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII
Nature Communications
title 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII
title_full 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII
title_fullStr 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII
title_full_unstemmed 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII
title_short 12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII
title_sort 12 13 dihome protects against the age related decline in cardiovascular function via attenuation of camkii
url https://doi.org/10.1038/s41467-025-62474-7
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