Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry
Summary: Immature granulocytes (iGRANs) circulating in the peripheral blood of patients with a chronic myeloid malignancy, such as chronic myelomonocytic leukemia, are immunosuppressive cells that promote disease progression. Here, we present a multiparameter flow cytometry-based protocol to quantif...
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004150 |
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| author | Margot Morabito Eric Solary Dorothée Selimoglu-Buet |
| author_facet | Margot Morabito Eric Solary Dorothée Selimoglu-Buet |
| author_sort | Margot Morabito |
| collection | DOAJ |
| description | Summary: Immature granulocytes (iGRANs) circulating in the peripheral blood of patients with a chronic myeloid malignancy, such as chronic myelomonocytic leukemia, are immunosuppressive cells that promote disease progression. Here, we present a multiparameter flow cytometry-based protocol to quantify iGRANs among peripheral blood mononucleated cells (PBMCs). We describe steps for collecting and labeling PBMCs. We describe the gating strategy to quantify iGRANs as a fraction of myeloid cells or as an absolute number of circulating cells, which supports patient stratification and monitoring.For complete details on the use and execution of this protocol, please refer to Deschamps et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-e914efcbbd764901a29aa94e3512c943 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-e914efcbbd764901a29aa94e3512c9432025-08-20T03:39:05ZengElsevierSTAR Protocols2666-16672025-09-016310400910.1016/j.xpro.2025.104009Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometryMargot Morabito0Eric Solary1Dorothée Selimoglu-Buet2INSERM U1287, Gustave Roussy Cancer Center, Villejuif, France; Université Paris-Saclay, Faculté de Médecine, Le Kremlin-Bicêtre, France; Corresponding authorINSERM U1287, Gustave Roussy Cancer Center, Villejuif, France; Université Paris-Saclay, Faculté de Médecine, Le Kremlin-Bicêtre, FranceINSERM U1287, Gustave Roussy Cancer Center, Villejuif, France; Université Paris-Saclay, Faculté de Médecine, Le Kremlin-Bicêtre, France; Corresponding authorSummary: Immature granulocytes (iGRANs) circulating in the peripheral blood of patients with a chronic myeloid malignancy, such as chronic myelomonocytic leukemia, are immunosuppressive cells that promote disease progression. Here, we present a multiparameter flow cytometry-based protocol to quantify iGRANs among peripheral blood mononucleated cells (PBMCs). We describe steps for collecting and labeling PBMCs. We describe the gating strategy to quantify iGRANs as a fraction of myeloid cells or as an absolute number of circulating cells, which supports patient stratification and monitoring.For complete details on the use and execution of this protocol, please refer to Deschamps et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725004150CancerFlow CytometryImmunology |
| spellingShingle | Margot Morabito Eric Solary Dorothée Selimoglu-Buet Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry STAR Protocols Cancer Flow Cytometry Immunology |
| title | Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry |
| title_full | Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry |
| title_fullStr | Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry |
| title_full_unstemmed | Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry |
| title_short | Protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry |
| title_sort | protocol for quantification of circulating immature granulocytes in patients with a chronic myeloid malignancy using flow cytometry |
| topic | Cancer Flow Cytometry Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725004150 |
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