Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus
Inefficient crop phosphorus (P) use impacts global food security and P fertilizer use can be environmentally harmful. Lines homozygous for barley (<i>Hordeum vulgare</i> L.) <i>low phytic acid 1-1</i> (<i>lpa 1-1</i>) have yields equivalent to the wild type but ~1...
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2025-06-01
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| author | Beverly L. Agesa Victor Raboy Paul J. A. Withers Katherine A. Steele |
| author_facet | Beverly L. Agesa Victor Raboy Paul J. A. Withers Katherine A. Steele |
| author_sort | Beverly L. Agesa |
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| description | Inefficient crop phosphorus (P) use impacts global food security and P fertilizer use can be environmentally harmful. Lines homozygous for barley (<i>Hordeum vulgare</i> L.) <i>low phytic acid 1-1</i> (<i>lpa 1-1</i>) have yields equivalent to the wild type but ~15% less seed Total P (TP). The objective here was to identify second-site mutations in the <i>lpa1-1</i> background that condition a further reduction in seed TP, again with little impact on yield. A chemically mutagenized population was derived from <i>lpa 1-1</i> and screened to identify lines with seed TP reductions greater than 15% (as compared with wild-type) but with seed weights per plant within 80% of wild-type. Three M<sub>4</sub> lines were selected and evaluated in a greenhouse pot experiment. Plants were grown to maturity either on a soil with low soil P fertility (16 to 25 mg Olsen P L<sup>−1</sup>; Soil P Index 1) or with that soil supplemented (36 kg P ha<sup>−1</sup>) to provide optimal available soil P. Mean seed P reduction across the three lines and two soil P levels was 28%, a near doubling of the <i>lpa1-1</i> seed Total P reduction. When grown with optimal soil available P, no impact of these putative mutations on grain yield was observed. These findings suggest that the three <i>lpa 1-1</i>-derived mutant lines carry second-site mutations conferring substantially (~17%) greater decreases in seed TP than that conferred by <i>lpa 1-1</i>. If the putative mutations are confirmed to be heritable and to have negligible impact on yield, they could be used in breeding P-efficient barley cultivars as a step towards reducing regional and global P demand. |
| format | Article |
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| institution | DOAJ |
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| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-e9143148d7824ec9b19cdabe879b3ca12025-08-20T02:45:49ZengMDPI AGAgronomy2073-43952025-06-01157155010.3390/agronomy15071550Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total PhosphorusBeverly L. Agesa0Victor Raboy1Paul J. A. Withers2Katherine A. Steele3School of Natural and Environmental Sciences, Bangor University, Bangor LL57 2UW, UKUS Department of Agriculture (USDA), Agricultural Research Service (ARS), Portland, OR 97223, USALancaster Environment Centre, University of Lancaster, Lancaster LA1 4YQ, UKSchool of Natural and Environmental Sciences, Bangor University, Bangor LL57 2UW, UKInefficient crop phosphorus (P) use impacts global food security and P fertilizer use can be environmentally harmful. Lines homozygous for barley (<i>Hordeum vulgare</i> L.) <i>low phytic acid 1-1</i> (<i>lpa 1-1</i>) have yields equivalent to the wild type but ~15% less seed Total P (TP). The objective here was to identify second-site mutations in the <i>lpa1-1</i> background that condition a further reduction in seed TP, again with little impact on yield. A chemically mutagenized population was derived from <i>lpa 1-1</i> and screened to identify lines with seed TP reductions greater than 15% (as compared with wild-type) but with seed weights per plant within 80% of wild-type. Three M<sub>4</sub> lines were selected and evaluated in a greenhouse pot experiment. Plants were grown to maturity either on a soil with low soil P fertility (16 to 25 mg Olsen P L<sup>−1</sup>; Soil P Index 1) or with that soil supplemented (36 kg P ha<sup>−1</sup>) to provide optimal available soil P. Mean seed P reduction across the three lines and two soil P levels was 28%, a near doubling of the <i>lpa1-1</i> seed Total P reduction. When grown with optimal soil available P, no impact of these putative mutations on grain yield was observed. These findings suggest that the three <i>lpa 1-1</i>-derived mutant lines carry second-site mutations conferring substantially (~17%) greater decreases in seed TP than that conferred by <i>lpa 1-1</i>. If the putative mutations are confirmed to be heritable and to have negligible impact on yield, they could be used in breeding P-efficient barley cultivars as a step towards reducing regional and global P demand.https://www.mdpi.com/2073-4395/15/7/1550<i>Hordeum vulgare</i>phosphorusphytatenutrient use efficiencygrain |
| spellingShingle | Beverly L. Agesa Victor Raboy Paul J. A. Withers Katherine A. Steele Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus Agronomy <i>Hordeum vulgare</i> phosphorus phytate nutrient use efficiency grain |
| title | Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus |
| title_full | Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus |
| title_fullStr | Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus |
| title_full_unstemmed | Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus |
| title_short | Putative Second-Site Mutations in the Barley Low Phytic Acid 1-1 (lpa 1-1) Genetic Background Further Reduce Seed Total Phosphorus |
| title_sort | putative second site mutations in the barley low phytic acid 1 1 lpa 1 1 genetic background further reduce seed total phosphorus |
| topic | <i>Hordeum vulgare</i> phosphorus phytate nutrient use efficiency grain |
| url | https://www.mdpi.com/2073-4395/15/7/1550 |
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