Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat

Background. The influence of low doses of aflatoxin B1 (AFB1) and partial hepatectomy (PH) on glutathione-S-transferase (GST) activity was studied in the plasma and liver of the rat. Methods. The animals were divided into four groups. The first (I) and the second (II) group were treated with AFB1 fr...

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Main Authors: Strelić Nataša J., Saičić Zorica S., Magić Zvonko M., Spasić Mihajlo B., Trutić Nataša V., Krtolica-Žikić Koviljka V.
Format: Article
Language:English
Published: Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade 2003-01-01
Series:Vojnosanitetski Pregled
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Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2003/0042-84500304415S.pdf
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author Strelić Nataša J.
Saičić Zorica S.
Magić Zvonko M.
Spasić Mihajlo B.
Trutić Nataša V.
Krtolica-Žikić Koviljka V.
author_facet Strelić Nataša J.
Saičić Zorica S.
Magić Zvonko M.
Spasić Mihajlo B.
Trutić Nataša V.
Krtolica-Žikić Koviljka V.
author_sort Strelić Nataša J.
collection DOAJ
description Background. The influence of low doses of aflatoxin B1 (AFB1) and partial hepatectomy (PH) on glutathione-S-transferase (GST) activity was studied in the plasma and liver of the rat. Methods. The animals were divided into four groups. The first (I) and the second (II) group were treated with AFB1 freshly dissolved in dimethyl sulphoxide (DMSO), and administered as a single intraperitoneal dose of 50 mg/rat 24 hrs after the rats had undergone either sham operation or, 40% PH, respectively. The third group (III) of animals was treated with a total dose of 1 mg AFB1 - 5 days per week during a period of 8 weeks. The non-treated animals were used as controls (C). Results. We observed a significant increase of GST activity in the plasma of all experimental groups compared to the controls (C), (p<0.02 to p<0.005). In the liver, the GST activity of all experimental groups was also significantly increased, compared to the controls (from p<0.02 to p<0.005). Conclusion. The administration of both single and multiple doses of AFB1 led to long term increase of GST activity in the rat plasma and liver, and partial hepatectomy had no significant effect on this phenomenon.
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spelling doaj-art-e901a359daae424aa9204ad49d0cfd822025-08-20T03:24:38ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502003-01-0160441542010.2298/VSP0304415SAflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the ratStrelić Nataša J.Saičić Zorica S.Magić Zvonko M.Spasić Mihajlo B.Trutić Nataša V.Krtolica-Žikić Koviljka V.Background. The influence of low doses of aflatoxin B1 (AFB1) and partial hepatectomy (PH) on glutathione-S-transferase (GST) activity was studied in the plasma and liver of the rat. Methods. The animals were divided into four groups. The first (I) and the second (II) group were treated with AFB1 freshly dissolved in dimethyl sulphoxide (DMSO), and administered as a single intraperitoneal dose of 50 mg/rat 24 hrs after the rats had undergone either sham operation or, 40% PH, respectively. The third group (III) of animals was treated with a total dose of 1 mg AFB1 - 5 days per week during a period of 8 weeks. The non-treated animals were used as controls (C). Results. We observed a significant increase of GST activity in the plasma of all experimental groups compared to the controls (C), (p<0.02 to p<0.005). In the liver, the GST activity of all experimental groups was also significantly increased, compared to the controls (from p<0.02 to p<0.005). Conclusion. The administration of both single and multiple doses of AFB1 led to long term increase of GST activity in the rat plasma and liver, and partial hepatectomy had no significant effect on this phenomenon.http://www.doiserbia.nb.rs/img/doi/0042-8450/2003/0042-84500304415S.pdfaflatoxinscarcinogensglutathione transferaseliverplasmarats
spellingShingle Strelić Nataša J.
Saičić Zorica S.
Magić Zvonko M.
Spasić Mihajlo B.
Trutić Nataša V.
Krtolica-Žikić Koviljka V.
Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat
Vojnosanitetski Pregled
aflatoxins
carcinogens
glutathione transferase
liver
plasma
rats
title Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat
title_full Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat
title_fullStr Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat
title_full_unstemmed Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat
title_short Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat
title_sort aflatoxin b1 induced changes of glutathione s transferase activity in the plasma and liver of the rat
topic aflatoxins
carcinogens
glutathione transferase
liver
plasma
rats
url http://www.doiserbia.nb.rs/img/doi/0042-8450/2003/0042-84500304415S.pdf
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