Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain
The mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and p...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8651453 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551014912229376 |
|---|---|
| author | Thi Phuong Lan Nguyen Van Tuyen Anh Nguyen Thi Thanh Trung Do Trung Nguyen Quang Quoc Long Pham Tat Thanh Le |
| author_facet | Thi Phuong Lan Nguyen Van Tuyen Anh Nguyen Thi Thanh Trung Do Trung Nguyen Quang Quoc Long Pham Tat Thanh Le |
| author_sort | Thi Phuong Lan Nguyen |
| collection | DOAJ |
| description | The mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. The total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. The composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. The anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time. The anti-inflammatory activity of the total and polar lipids had IC50 values of 71.5 and 68.6 μg/mL, respectively. The crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC50 values ranging from 85.4 to 95.8 μg/mL. |
| format | Article |
| id | doaj-art-e8fad84636334c638b36772623ab66d1 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-e8fad84636334c638b36772623ab66d12025-02-03T06:05:13ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/86514538651453Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosainThi Phuong Lan Nguyen0Van Tuyen Anh Nguyen1Thi Thanh Trung Do2Trung Nguyen Quang3Quoc Long Pham4Tat Thanh Le5Graduate University of Sciences and Technology, Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Cau Giay, Hanoi, VietnamInstitute of Natural Products Chemistry, VAST, 18-Hoang Quoc Viet, Cau Giay, Hanoi, VietnamInstitute of Natural Products Chemistry, VAST, 18-Hoang Quoc Viet, Cau Giay, Hanoi, VietnamCenter for Research and Technology Transfer, VAST, 18-Hoang Quoc Viet, Cau Giay, Hanoi, VietnamInstitute of Natural Products Chemistry, VAST, 18-Hoang Quoc Viet, Cau Giay, Hanoi, VietnamGraduate University of Sciences and Technology, Vietnam Academy of Science and Technology (VAST), 18-Hoang Quoc Viet, Cau Giay, Hanoi, VietnamThe mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. The total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. The composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. The anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time. The anti-inflammatory activity of the total and polar lipids had IC50 values of 71.5 and 68.6 μg/mL, respectively. The crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC50 values ranging from 85.4 to 95.8 μg/mL.http://dx.doi.org/10.1155/2020/8651453 |
| spellingShingle | Thi Phuong Lan Nguyen Van Tuyen Anh Nguyen Thi Thanh Trung Do Trung Nguyen Quang Quoc Long Pham Tat Thanh Le Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain Journal of Chemistry |
| title | Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain |
| title_full | Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain |
| title_fullStr | Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain |
| title_full_unstemmed | Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain |
| title_short | Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain |
| title_sort | fatty acid composition phospholipid molecules and bioactivities of lipids of the mud crab scylla paramamosain |
| url | http://dx.doi.org/10.1155/2020/8651453 |
| work_keys_str_mv | AT thiphuonglannguyen fattyacidcompositionphospholipidmoleculesandbioactivitiesoflipidsofthemudcrabscyllaparamamosain AT vantuyenanhnguyen fattyacidcompositionphospholipidmoleculesandbioactivitiesoflipidsofthemudcrabscyllaparamamosain AT thithanhtrungdo fattyacidcompositionphospholipidmoleculesandbioactivitiesoflipidsofthemudcrabscyllaparamamosain AT trungnguyenquang fattyacidcompositionphospholipidmoleculesandbioactivitiesoflipidsofthemudcrabscyllaparamamosain AT quoclongpham fattyacidcompositionphospholipidmoleculesandbioactivitiesoflipidsofthemudcrabscyllaparamamosain AT tatthanhle fattyacidcompositionphospholipidmoleculesandbioactivitiesoflipidsofthemudcrabscyllaparamamosain |