The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia

Aim of the study. Aim of the study was to estimate the occurrence of pathogenic mutations in the BRCA1 gene in Russian breast cancer patients.Material and methods. Complete coding sequence of the BRCA1 gene of 445 early onset  breast cancer patients (under 40 years) from Novosibirsk region (Russia)...

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Main Authors: M. S. Anisimenko, G. A. Paul, A. E. Kozyakov, N. I. Gutkina, D. A. Berdyugina, A. Yu. Garanin, A. V. Butorina, E. V. Gornostaeva, K. F. Khafizov, Yu. V. Vyatkin, D. N. Shtokalo, S. P. Kovalenko
Format: Article
Language:Russian
Published: Russian Academy of Sciences, Tomsk National Research Medical Center 2018-09-01
Series:Сибирский онкологический журнал
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Online Access:https://www.siboncoj.ru/jour/article/view/816
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author M. S. Anisimenko
G. A. Paul
A. E. Kozyakov
N. I. Gutkina
D. A. Berdyugina
A. Yu. Garanin
A. V. Butorina
E. V. Gornostaeva
K. F. Khafizov
Yu. V. Vyatkin
D. N. Shtokalo
S. P. Kovalenko
author_facet M. S. Anisimenko
G. A. Paul
A. E. Kozyakov
N. I. Gutkina
D. A. Berdyugina
A. Yu. Garanin
A. V. Butorina
E. V. Gornostaeva
K. F. Khafizov
Yu. V. Vyatkin
D. N. Shtokalo
S. P. Kovalenko
author_sort M. S. Anisimenko
collection DOAJ
description Aim of the study. Aim of the study was to estimate the occurrence of pathogenic mutations in the BRCA1 gene in Russian breast cancer patients.Material and methods. Complete coding sequence of the BRCA1 gene of 445 early onset  breast cancer patients (under 40 years) from Novosibirsk region (Russia) were analyzed by  targeted Next Generation Sequencing (NGS) using Ion Torrent platform. Results. Forty (9%) carriers of various pathogenic mutations were revealed. Thirty five (7,9%) patients  carried 5382insC mutation, described earlier as a founder mutation for Slavic population.  Five (1.1%) patients carried various pathogenic mutations, namely C61G, 462delCC, E143X,  4153delA, and IVS18+1G>T. Besides, 29 genetic variants with no clinical significance or with  unknown clinical significance were detected in BRCA1 gene among 445 early onset breast  cancer patients. Conclusions. Data on the frequency of genetic variations in the BRCA1 gene among early onset breast cancer patients in the Novosibirsk Region (Russia) were  obtained. Proportion of the 5382insC mutation is 87.5% of all pathogenic mutations in the BRCA1 gene found in patients.
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spelling doaj-art-e8f4586fb9f3475097456d7988c4d2782025-08-20T03:35:48ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682018-09-01174535810.21294/1814-4861-2018-17-4-53-58562The spectrum of BRCA1 gene mutations in early onset breast cancer patients from RussiaM. S. Anisimenko0G. A. Paul1A. E. Kozyakov2N. I. Gutkina3D. A. Berdyugina4A. Yu. Garanin5A. V. Butorina6E. V. Gornostaeva7K. F. Khafizov8Yu. V. Vyatkin9D. N. Shtokalo10S. P. Kovalenko11Federal Research Center of Fundamental and Translational Medicine BioLink LtdBioLink LtdNovosibirsk Regional Oncological ClinicFederal Research Center of Fundamental and Translational MedicineFederal Research Center of Fundamental and Translational Medicine BioLink LtdFederal Research Center of Fundamental and Translational Medicine BioLink LtdFederal Research Center of Fundamental and Translational Medicine BioLink LtdFederal Research Center of Fundamental and Translational Medicine BioLink LtdCentral Research Institute of EpidemiologyAcademGene LLCAcademGene LLC A.P. Ershov Institute of Informatics SystemsFederal Research Center of Fundamental and Translational Medicine BioLink Ltd Novosibirsk State UniversityAim of the study. Aim of the study was to estimate the occurrence of pathogenic mutations in the BRCA1 gene in Russian breast cancer patients.Material and methods. Complete coding sequence of the BRCA1 gene of 445 early onset  breast cancer patients (under 40 years) from Novosibirsk region (Russia) were analyzed by  targeted Next Generation Sequencing (NGS) using Ion Torrent platform. Results. Forty (9%) carriers of various pathogenic mutations were revealed. Thirty five (7,9%) patients  carried 5382insC mutation, described earlier as a founder mutation for Slavic population.  Five (1.1%) patients carried various pathogenic mutations, namely C61G, 462delCC, E143X,  4153delA, and IVS18+1G>T. Besides, 29 genetic variants with no clinical significance or with  unknown clinical significance were detected in BRCA1 gene among 445 early onset breast  cancer patients. Conclusions. Data on the frequency of genetic variations in the BRCA1 gene among early onset breast cancer patients in the Novosibirsk Region (Russia) were  obtained. Proportion of the 5382insC mutation is 87.5% of all pathogenic mutations in the BRCA1 gene found in patients.https://www.siboncoj.ru/jour/article/view/816brca1 genemutationearly onset breast cancerhereditary cancerngstargeted sequencing
spellingShingle M. S. Anisimenko
G. A. Paul
A. E. Kozyakov
N. I. Gutkina
D. A. Berdyugina
A. Yu. Garanin
A. V. Butorina
E. V. Gornostaeva
K. F. Khafizov
Yu. V. Vyatkin
D. N. Shtokalo
S. P. Kovalenko
The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia
Сибирский онкологический журнал
brca1 gene
mutation
early onset breast cancer
hereditary cancer
ngs
targeted sequencing
title The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia
title_full The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia
title_fullStr The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia
title_full_unstemmed The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia
title_short The spectrum of BRCA1 gene mutations in early onset breast cancer patients from Russia
title_sort spectrum of brca1 gene mutations in early onset breast cancer patients from russia
topic brca1 gene
mutation
early onset breast cancer
hereditary cancer
ngs
targeted sequencing
url https://www.siboncoj.ru/jour/article/view/816
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