The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves

<b>Objectives</b>: This article presents a study evaluating the antibody levels against infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea (BVD) in Kazakh Whiteheaded calves born to dams immunized with an experimental inactivated combined vaccine against these infections....

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Main Authors: Yerbol Bulatov, Alina Kurmasheva, Zhanat Amanova, Ruslan Abitaev, Zhanna Sametova, Asselya Kyrgyzbayeva, Zhanat Kondybaeva, Sholpan Turyskeldi, Abdurakhman Ussembay, Dariya Toktyrova, Dana Mazbayeva, Yeraly Shayakhmetov, Aslan Kerimbayev, Damir Khussainov, Ma Wentao, Aralbek Rsaliyev, Yergali Abduraimov
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Vaccines
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Online Access:https://www.mdpi.com/2076-393X/13/4/408
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author Yerbol Bulatov
Alina Kurmasheva
Zhanat Amanova
Ruslan Abitaev
Zhanna Sametova
Asselya Kyrgyzbayeva
Zhanat Kondybaeva
Sholpan Turyskeldi
Abdurakhman Ussembay
Dariya Toktyrova
Dana Mazbayeva
Yeraly Shayakhmetov
Aslan Kerimbayev
Damir Khussainov
Ma Wentao
Aralbek Rsaliyev
Yergali Abduraimov
author_facet Yerbol Bulatov
Alina Kurmasheva
Zhanat Amanova
Ruslan Abitaev
Zhanna Sametova
Asselya Kyrgyzbayeva
Zhanat Kondybaeva
Sholpan Turyskeldi
Abdurakhman Ussembay
Dariya Toktyrova
Dana Mazbayeva
Yeraly Shayakhmetov
Aslan Kerimbayev
Damir Khussainov
Ma Wentao
Aralbek Rsaliyev
Yergali Abduraimov
author_sort Yerbol Bulatov
collection DOAJ
description <b>Objectives</b>: This article presents a study evaluating the antibody levels against infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea (BVD) in Kazakh Whiteheaded calves born to dams immunized with an experimental inactivated combined vaccine against these infections. The vaccine formulation includes the strains “R-93” (IBR) and “Oregon C<sub>24</sub>V” (BVD), which are preserved in the microorganism collection of the Research Institute for Biological Safety Problems. <b>Methods</b>: To assess the immune response in newborn calves, blood serum samples were collected before the first intake of colostrum, followed by weekly sampling for 28 weeks post-birth. The antibody response was determined using a virus neutralization assay on MDBK cell cultures and lamb testicle cell cultures. <b>Results</b>: The results demonstrated that the protective antibody level against the IBR virus (≥2 log<sub>2</sub>) persisted for up to 25 weeks, while the protective level against the BVD virus (≥3 log<sub>2</sub>) remained for 23 weeks. Based on these findings, the vaccine was deemed safe, as it did not induce abortions or clinical manifestations of the diseases. The overall duration of the colostral immunity in calves against the IBR and BVD viruses reached 23 weeks. <b>Conclusions</b>: Therefore, it is recommended that Kazakh Whiteheaded calves be vaccinated with the associated inactivated vaccine against infectious bovine rhinotracheitis and bovine viral diarrhea no earlier than 23 weeks of age.
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spelling doaj-art-e8e9f547bd4840aaa8bdb78c634edd152025-08-20T02:25:08ZengMDPI AGVaccines2076-393X2025-04-0113440810.3390/vaccines13040408The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded CalvesYerbol Bulatov0Alina Kurmasheva1Zhanat Amanova2Ruslan Abitaev3Zhanna Sametova4Asselya Kyrgyzbayeva5Zhanat Kondybaeva6Sholpan Turyskeldi7Abdurakhman Ussembay8Dariya Toktyrova9Dana Mazbayeva10Yeraly Shayakhmetov11Aslan Kerimbayev12Damir Khussainov13Ma Wentao14Aralbek Rsaliyev15Yergali Abduraimov16Research Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy 080409, KazakhstanDepartment of Veterinary and Zooengineering, Kazakh National Agrarian Research University, Almaty 050010, KazakhstanCollege of Veterinary Medicine, Northwest Agriculture and Forestry University, Xianyang 712100, ChinaNational Holding “QazBioPharm”, Astana 010000, KazakhstanNational Holding “QazBioPharm”, Astana 010000, Kazakhstan<b>Objectives</b>: This article presents a study evaluating the antibody levels against infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea (BVD) in Kazakh Whiteheaded calves born to dams immunized with an experimental inactivated combined vaccine against these infections. The vaccine formulation includes the strains “R-93” (IBR) and “Oregon C<sub>24</sub>V” (BVD), which are preserved in the microorganism collection of the Research Institute for Biological Safety Problems. <b>Methods</b>: To assess the immune response in newborn calves, blood serum samples were collected before the first intake of colostrum, followed by weekly sampling for 28 weeks post-birth. The antibody response was determined using a virus neutralization assay on MDBK cell cultures and lamb testicle cell cultures. <b>Results</b>: The results demonstrated that the protective antibody level against the IBR virus (≥2 log<sub>2</sub>) persisted for up to 25 weeks, while the protective level against the BVD virus (≥3 log<sub>2</sub>) remained for 23 weeks. Based on these findings, the vaccine was deemed safe, as it did not induce abortions or clinical manifestations of the diseases. The overall duration of the colostral immunity in calves against the IBR and BVD viruses reached 23 weeks. <b>Conclusions</b>: Therefore, it is recommended that Kazakh Whiteheaded calves be vaccinated with the associated inactivated vaccine against infectious bovine rhinotracheitis and bovine viral diarrhea no earlier than 23 weeks of age.https://www.mdpi.com/2076-393X/13/4/408infectious bovine rhinotracheitisbovine viral diarrheapregnant cowscolostral immunity
spellingShingle Yerbol Bulatov
Alina Kurmasheva
Zhanat Amanova
Ruslan Abitaev
Zhanna Sametova
Asselya Kyrgyzbayeva
Zhanat Kondybaeva
Sholpan Turyskeldi
Abdurakhman Ussembay
Dariya Toktyrova
Dana Mazbayeva
Yeraly Shayakhmetov
Aslan Kerimbayev
Damir Khussainov
Ma Wentao
Aralbek Rsaliyev
Yergali Abduraimov
The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves
Vaccines
infectious bovine rhinotracheitis
bovine viral diarrhea
pregnant cows
colostral immunity
title The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves
title_full The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves
title_fullStr The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves
title_full_unstemmed The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves
title_short The Influence of the Associated Inactivated Vaccine Against Infectious Rhinotracheitis and Bovine Viral Diarrhea on the Formation and Duration of Colostral Immunity in Kazakh Whiteheaded Calves
title_sort influence of the associated inactivated vaccine against infectious rhinotracheitis and bovine viral diarrhea on the formation and duration of colostral immunity in kazakh whiteheaded calves
topic infectious bovine rhinotracheitis
bovine viral diarrhea
pregnant cows
colostral immunity
url https://www.mdpi.com/2076-393X/13/4/408
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